Stabilizer as well as preparation method and application thereof

Abstract

The invention discloses a stabilizer and a preparation method and application thereof. The stabilizer is prepared from distilled water, ethylene glycol, absolute ethyl alcohol, polyethylene glycol, tartrate and a PH regulator. The mass percent of each component is 91.7 to 96.7 percent of distilled water, 2 to 5 percent of ethylene glycol, 0.1 to 2 percent of absolute ethyl alcohol, 0.01 to 0.2 percent of polyethylene glycol, 0.01 to 0.3 percent of tartrate and 0.05 to 0.15 percent of pH regulator. The preparation method comprises the following steps: S1, weighing each component material according to metering; S2, adding all the materials into a beaker, fully stirring the materials in the beaker during adding, and obtaining a mixed solution; S3, slowly adding a pH regulator into the mixed solution, and regulating the pH value of the mixed solution to 2-4; and S4, filtering the mixed solution with the adjusted pH value to obtain filtrate, namely the stabilizer. The stabilizer is used for soaking the section and is applied between a hydration operation step and a hematoxylin dyeing operation step in a cell nucleus dyeing process.

Description

technical field [0001] The invention relates to the field of biochemistry, in particular to a stabilizer and its preparation method and application. Background technique [0002] Hematoxylin-eosin staining (hematoxylin-eosinstaining), referred to as HE staining, is one of the commonly used staining methods in paraffin section technology. The hematoxylin staining solution is alkaline, which mainly makes the chromatin in the nucleus and the nucleic acid in the cytoplasm stain purple blue; eosin is an acid dye, which mainly makes the components in the cytoplasm and extracellular matrix red. HE staining is the most basic and widely used technical method in the teaching and scientific research of histology, embryology and pathology. [0003] The operating steps in the hematoxylin-eosin staining method mainly include sectioning—baking—dewaxing—hydration—hematoxylin staining—differentiation—bluening—eosin staining—dehydration—clearing—sealing; After dewaxing and hydration, it dir...

AI Technical Summary

Problems solved by technology

[0003] The operating steps in the hematoxylin-eosin staining method mainly include sectioning—baking—dewaxing—hydration—hematoxylin staining—differentiation—bluening—eosin staining—dehydration—clearing—sealing; After dewaxing and hydration, it directly enters the hematoxylin dye solution for dyeing. On the one hand, because the last step of hydration is through flowing tap water, and the tap water contains strong oxidizing hypochlorous acid, which accumulates continuously and accelerates the hematoxylin dye solution. The hematoxylin is oxidized into hematoxylin, which shortens the service life of the hematoxylin staining solution and significantly increases the amount of hematoxylin staining solution. On the other hand, when the sliced ​​tissue enters the complex dyeing solution In the environment, there will be some differences in the staining effect of different tissues, and it may even be prone to decolorization after staining, which seriously reduces the staining effect of the nucleus. Therefore, it is necessary to improve the above problems