Brassica rapa regeneration system establishment and anther culture method

A cabbage-type rapeseed and a technology for establishing a method are applied in the fields of establishment of a cabbage-type rapeseed regeneration system and anther culture, which can solve the problems of lack of reproducibility, influence on anther callus induction rate, low callus induction and the like, and achieve the growth rate Inhibition, good effect, consistent plant growth effect

Pending Publication Date: 2021-08-24
GANSU AGRI UNIV
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Problems solved by technology

[0007] The low callus induction is mainly genotype-dependent, and the anther culture of different crops is not reproducible, and there may be differences in chemical components that affect the callus induction rate of anthers

Method used

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  • Brassica rapa regeneration system establishment and anther culture method
  • Brassica rapa regeneration system establishment and anther culture method
  • Brassica rapa regeneration system establishment and anther culture method

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Embodiment Construction

[0037] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0038] Aiming at the problems existing in the prior art, the present invention provides a method for establishing a regeneration system of Chinese cabbage-type rape and cultivating anthers. The present invention will be described in detail below in conjunction with the accompanying drawings.

[0039] Such as figure 1 As shown, the method for establishing the Chinese cabbage type rape regeneration system provided by the embodiments of the present invention includes:

[0040] S101, seed soaking treatment;

[0041] S102, seed disinfection;

[0042] S103, seed dark treatment culture;

[0043] S104, cutting the explant;

[00...

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Abstract

The invention belongs to the technical field of plant culture, and discloses a brassica rapa regeneration system establishment and anther culture method. The method comprises the following steps: analyzing the influence of different soaking times, different disinfection modes and dark culture at different times on seed germination indexes and growth indexes through a single factor experiment to obtain the optimal soaking time, disinfection mode and dark treatment time; comparing the influence of different hormone combinations on the induction and differentiation rates of different explant calluses, so as to screen out the optimal differentiation culture medium. According to the influence of different soaking times on the seed germination indexes, the optimal soaking time of soaking in sterile water for 6 hours is obtained, so that the seed dormancy can be broken, and the rooting and germination of the seeds can be accelerated; and different disinfection modes have different influences on the germination indexes and growth indexes of the explants, and according to the method, the seeds soaked for 6 hours are disinfected with a 3% sodium hypochlorite solution for 10 minutes and then are subjected to dark culture for 3 days, so that the aim of consistent seed germination and plant growth can be achieved.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a method for establishing a regeneration system of Chinese cabbage-type rape and cultivating anthers. Background technique [0002] At present, rape belongs to the cultivated subpopulation of Brassica (B. campestris L.) in the family Cruciferae (Cruciferae). It is one of the important oil crops in my country and the second largest oil crop in the world. Among the oil crops planted in my country, rapeseed is the variety with the greatest potential and hope, and it is the best choice to ensure and improve the self-sufficiency rate of edible oil in China. As a major oil production and consumption country in the world, China’s self-sufficiency rate is less than 35%. Experts focus on improving the yield and quality of rapeseed, and cultivating new varieties of rapeseed that are resistant to disease and stress. Tissue culture was performed as explants. Now a relat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 王旺田武军艳马骊孙万仓李学才杨晨方彦刘丽君浦媛媛刘博
Owner GANSU AGRI UNIV
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