Molecular marker related to lung cancer proliferation and metastasis and application thereof

A lung cancer cell and lung cancer diagnosis technology, applied in the field of molecular markers, to achieve the effect of inhibiting the proliferation of lung cancer cells and increasing the expression level
CN113293208AActive Publication Date: 2021-08-24CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2021-08-24

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Abstract

The invention provides a molecular marker related to lung cancer proliferation and metastasis and application of the molecular marker. A gene editing technology CRISPR / Cas9 system is adopted, it is found that in a human lung cancer cell line, the TMEM116 gene is closely related to metastasis and proliferation of lung cancer, the TMEM116 gene knockout sgRNA is designed, and the lung cancer cell line with the TMEM116 gene knocked down is obtained through a lipid transfection method. On the basis, in-vitro and in-vivo experiments of mice find that the carcinogenic ability and the metastasis ability of TMEM116 knocked-down lung cancer cells are obviously reduced, the relationship between the TMEM116 gene and the lung cancer is clarified, the TMEM116 gene is found to be used as a molecular marker related to lung cancer proliferation and metastasis and used for diagnosis of the lung cancer, and a new method is provided for diagnosis and treatment of the lung cancer.
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Description

technical field

[0001] The invention relates to the field of medical molecular biology, in particular to the technical field of lung cancer diagnosis, and in particular to molecular markers related to proliferation and metastasis of lung cancer and applications thereof. Background technique

[0002] Due to the stimulation of smoking, air pollution and some chemical factors, the normal lung cells of the human body are damaged, and gene mutations are prone to occur in the process of body damage repair, which in turn leads to the occurrence of tumors and cancers. Currently, lung cancer is also one of the most common malignancies, accounting for 18.4% of cancer deaths worldwide. Lung cancer is a highly complex tumor with a generally severe prognosis, with a 5-year survival rate of only 15%. In addition, under the promotion of metabolic changes and immune evasion, lung cancer cells can easily metastasize to other organs through blood vessels and lymphatic vessels, forming second...

Claims

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