A kind of SNP marker related to pig backfat thickness and its utilization method

A technology of pigs with thick backfat and breeding pigs, which is applied in the field of pig breeding, can solve the problems of large confidence intervals, etc., and achieve the effects of improving economic benefits, increasing the speed of breeding, and enriching molecular markers

Active Publication Date: 2022-07-08
AGRI GENOMICS INST CHINESE ACADEMY OF AGRI SCI
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Studies have found that genetic factors are the most important reason for pig backfat thickness. However, the number of quantitative trait loci (QTL) related to pig backfat thickness found so far is relatively large, and the confidence interval is large, so it is difficult to pass the QTL Mapping identifies causal mutation affecting backfat thickness

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  • A kind of SNP marker related to pig backfat thickness and its utilization method
  • A kind of SNP marker related to pig backfat thickness and its utilization method
  • A kind of SNP marker related to pig backfat thickness and its utilization method

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Experimental program
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Embodiment approach

[0024] According to a preferred embodiment of the present invention, the SNP marker located at 312, 813, 451 bp of chromosome 1 of the pig reference genome Sscrofa10.2 version sequence has a C / T polymorphism.

[0025] Among them, the two alleles of the SNP locus are C and T, the gene frequency of C is 0.9773, the gene frequency of T is 0.0627, and C is the dominant allele.

[0026] In a further preferred embodiment, the genotype of the SNP site located at 312, 813, 451 bp of chromosome 1 of the swine reference genome Sscrofa10.2 version sequence is TT genotype pigs have more genotypes than TC and CC pigs Low backfat thickness.

[0027] In the present invention, there are three genotypes corresponding to the SNP site located at the 312, 813, 451 bp of chromosome 1 of the pig reference genome Sscrofa10.2 version sequence, which are TT, TC and CC, respectively, and the TT genotype is that the site is Homozygous for base T, TC genotype is heterozygote for this site, CC genotype i...

Embodiment 1

[0069] Example 1 Acquisition of SNP site

[0070] 1. Experimental group

[0071] The pig population used in this example is 1148 pigs from the basic sow farm of Shenyuan pig in Hebei, including 588 large white pigs, 371 landrace pigs and 189 Duroc pigs.

[0072] 2. Measurement and correction of backfat thickness

[0073] When the individual body weight of the pig is in the range of 85-105kg, the backfat thickness of the living body is measured, and the backfat thickness at the 3rd to 4th intercostal space from the bottom is measured by B-ultrasound scanning, in millimeters; finally, the weight of pigs up to 100kg is calculated according to the following correction formula The in vivo backfat thickness:

[0074] The relationship between the corrected backfat thickness and the measured backfat thickness is:

[0075] Corrected backfat thickness = measured backfat thickness×CF (wherein: CF=A÷{A+[B×(measured weight-100)]});

[0076] Wherein, sow CF value=(measured body weight...

Embodiment 2

[0100] Example 2 Correlation results between different genotypes of SNP loci and backfat thickness

[0101] Take the genomic DNA of the pig population in Example 1, use Neogen_POR80K of Neogen Corporation to detect the genotype of each individual, extract the sequence of the polymorphic site by R language, and count the genotype frequency and gene frequency distribution of the SNP site, The results are shown in Table 2:

[0102] Table 2

[0103]

[0104] It can be seen from Table 2 that C is the dominant allele, and the CC genotype is the dominant genotype of the experimental population.

[0105] The Kruskal-Wallis method was used to test the significance of differences between genotype data and phenotype data using Rstudio software, where P-value<0.05 indicated a significant difference. The correlation analysis results are shown in Table 3:

[0106] table 3

[0107]

[0108] It can be seen from Table 3 that the corrected backfat thickness of the three genotypes of t...

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Abstract

The invention discloses a SNP marker significantly related to pig backfat thickness and a utilization method thereof. The SNP marker is located at the 312, 813, 451 bp of chromosome 1 of the pig reference genome Sscrofa10.2 version sequence, and the specific C / T polymorphism, Pigs with TT genotype at this locus had lower backfat thickness than pigs with TC and CC genotypes. The SNP markers that are significantly related to pig backfat thickness provided by the invention can be used to identify or assist in identifying individuals with lower pig backfat thickness, enrich the molecular markers of assisted breeding, increase the selection speed of excellent pig breeds, and speed up It accelerates the pace of molecular breeding of pigs, and at the same time provides a feasible solution for the breeding of pigs with low backfat thickness, which improves the economic benefits of pig production enterprises.

Description

technical field [0001] The invention belongs to the technical field of pig breeding, and in particular relates to a SNP marker related to pig backfat thickness and a utilization method thereof. Background technique [0002] The growth performance of pigs is mainly reflected in the aspects of muscle content, fat content, pork quality and feed utilization. In the process of modern animal husbandry, backfat thickness is an important in vivo performance measurement index. This trait can affect not only growth traits related to lean percentage but also reproductive traits related to litter size. At the same time, in the genetic improvement of important economic traits of pigs, backfat thickness is also an important reference index to measure the quality of pig breeds, and is often used for breeding good breeds. [0003] Studies have found that genetic factors are the most important factor affecting pig backfat thickness. However, the number of quantitative trait loci (QTL) foun...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156Y02P60/87
Inventor 唐中林范新浩易国强王斌虎
Owner AGRI GENOMICS INST CHINESE ACADEMY OF AGRI SCI
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