Microcell sample collection device for tissue slices

Abstract

The invention discloses a microcell sample collection device for tissue slices, which comprises a bearing platform, a sampling device, an inverted microscopic imaging module and a lateral microscopic imaging module, the sampling device is arranged above the bearing platform, the inverted microscopic imaging module is arranged below the bearing platform, the lateral microscopic imaging module is arranged on the side surface of the bearing platform, and the bearing platform comprises an electric platform. A tissue slice and a sample collection container are placed on the electric platform, the tissue slice is attached to a glass slide coated with a buffer layer, the tissue slice is comprehensively scanned by means of an inverted microscopic imaging module, an interested cell area is selected, and the site is moved to the position under a sampling microneedle; and an electric micro-operation system is used for controlling a micro-needle to descend, and the contact condition of the tip of the micro-needle and the tissue slice is observed in real time in the lateral micro-imaging module until the sampling micro-needle pierces the buffer layer and ensures that the micro-area of the site is cut off. According to the invention, in-situ acquisition of the microcell sample from the tissue slice can be realized, and high-quality RNA, DNA and protein can be obtained for downstream analysis.

Description

Technical field [0001] The present invention relates to a sample collection device, more particularly to a method for the micro-region tissue slice sample collection device. Background technique [0002] Cellular heterogeneity is a universal biological phenomenon, separated from the complex organisms in a particular anatomical region and the cell is to understand the premise of this heterogeneity has important implications for disease research, development and other organisms. At the same time, from multiple cells to co-operate cell subsets and their microenvironment also affect the expression heterogeneity, so that their spatial position of the background is crucial. Therefore, to obtain pure cell populations from heterogeneous tissue samples and to obtain spatial information is a prerequisite for molecular studies. Currently, laser capture microdissection is one of the applications of the isolated cell populations in tissue sections on the most widely used technology, we can lo...

AI Technical Summary

Problems solved by technology

However, when it takes local sampling, it has defects such as high cost of infrastructure, complicated processing, possible damage to genes, and single type of tissue slices, which limits its further promotion and research.

Images