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Multi-stage ketogenic diet formula for regulating intestinal flora and energy metabolism

A ketogenic diet and formula technology, applied in the food field, can solve the problems of increased low-density lipoprotein cholesterol concentration, nutritional deficiencies, poor compliance, etc.

Active Publication Date: 2021-09-10
WUXI PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the lack of whole grains, legumes, and fruits in the ketogenic diet formula, obese patients undergoing long-term ketogenic diet interventions may experience nutritional deficiencies, poor compliance, and increased LDL cholesterol concentrations

Method used

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  • Multi-stage ketogenic diet formula for regulating intestinal flora and energy metabolism
  • Multi-stage ketogenic diet formula for regulating intestinal flora and energy metabolism
  • Multi-stage ketogenic diet formula for regulating intestinal flora and energy metabolism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Effect of ketogenic diet formula on body weight, BMI, waist circumference of obese people

[0038] 1 Experimental method

[0039] Fifteen obese patients from Wuxi People's Hospital were selected, including 9 males and 7 females, with an age range of 18-47 years, a height range of 146-183cm, a weight range of 59.3-108.8kg, and a BMI range of 27.8-36.5kg / m 2 .

[0040] Diet formula: low-calorie ketogenic diet (according to the actual basal metabolism measured by the patient's InBody770 body composition meter, it is calibrated as total calories, and the energy supply ratio is 32% protein, 41% fat, and 27% carbohydrates (GI lower than 55)); Transition diet (according to the actual basal metabolism measured by the patient's InBody770 body composition meter multiplied by 1.1 and calibrated as total calories, the energy supply ratio is 29% protein, 42% fat, and 29% carbohydrate (GI lower than 65)); limit energy balance Diet (according to the basal metabolism actual...

Embodiment 2

[0060] Example 2 Effect of ketogenic diet on species composition of intestinal flora of obese people

[0061] 1. Stool sampling and storage

[0062] The stool samples of the obese patients in Example 1 were collected, and the collection time points were the day after the end of the introduction period and the day after the end of the intervention period. The collected samples were preserved in glycerol and stored at -80°C before processing.

[0063] 2. Fecal DNA extraction and sequencing

[0064] (1) Extraction of feces DNA: Take the evenly mixed feces and glycerol mixture, centrifuge to remove glycerol, and extract the bacterial genome from the feces samples according to the instructions of the FastDNASpin Kit For Feces kit produced by MP Company of the United States.

[0065] (2) PCR amplification and product purification: using the flora genome as a template, amplify the V3-V4 region fragment of 16S rDNA, the upstream primer is: CCTAYGGGRBGCASCAG, and the downstream prime...

Embodiment 3

[0091] Example 3 Effect of ketogenic diet on coexistence network of intestinal flora in obese people

[0092] 1. Experimental method

[0093] (1) Construction of intestinal flora coexistence network

[0094] In order to identify microbial interactions in the intestinal flora, correlation analysis based on species abundance composition data is a common method, but because the species abundance obtained by sequencing is not absolutely quantified, spurious relationships may occur. SparCC is a novel approach adapted to the characteristics of sequencing data, which can infer the relatedness between species and construct microbial species interaction network. Genera whose relative abundance was greater than 0.01% in at least one sample were selected for correlation analysis. Based on the screened genus-level species abundance tables, SparCC was used to calculate the species correlations before and after the ketogenic diet intervention, respectively. The original data set was resa...

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Abstract

The invention discloses a multi-stage ketogenic diet formula for regulating intestinal flora and energy metabolism, and belongs to the technical field of food. The formula can assist in improving obesity related indexes and improving obesity phenotypes. The BMI of obese people is obviously reduced (p is less than 0.05), and the BMI index of part of people is reduced from obesity to an overweight level. The weight of 86.7% of obese patients is at least reduced by 10% after weight losing intervention for three months, and the waistline is obviously reduced, which indicates that the abdominal fat mass of the obese patients is reduced. The intestinal flora of the obese patients can be adjusted, the function of the intestinal flora is influenced, the interaction network of the intestinal flora of the obese patients is changed, and the state of the intestinal flora of the obese patients tends to be healthy.

Description

technical field [0001] The invention relates to a multi-stage ketogenic diet formula for regulating intestinal flora and energy metabolism, which belongs to the technical field of food. Background technique [0002] The maintenance of energy homeostasis in the human body is achieved through the coordinated regulation of energy intake and energy expenditure. If energy intake exceeds energy expenditure, the body mass index (BMI) will increase, which will lead to obesity and metabolic disorders, and vice versa will lead to BMI decline and cause nutritional deficiencies. With changes in people's diet and lifestyle, the incidence of obesity continues to rise globally. According to the World Health Organization (WHO), the global prevalence of obesity almost tripled between 1975 and 2016. Obesity is often accompanied by excess fat in the liver and pancreas, which not only affects physical appearance and mental health, but is also a major risk factor for cardiovascular diseases, m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/00
CPCA23L33/40A23V2002/00A23V2200/32A23V2200/332
Inventor 吴文君王鸿超陈妮周群燕袁维维
Owner WUXI PEOPLES HOSPITAL
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