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Application of millet bran active polyphenol in preparation of product for preventing and treating atherosclerosis

A technology of atherosclerosis and rice bran, applied in the direction of application, function of food ingredients, medical preparations containing active ingredients, etc.

Pending Publication Date: 2021-09-10
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, domestic and foreign studies on the anti-atherosclerosis of polyphenols in millet bran have not been reported. Therefore, the present invention uses a kind of millet bran in the northern region as a material, and obtains an active polyphenol by optimizing the preparation process. The active polyphenol can effectively Inhibit the progression of atherosclerosis

Method used

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  • Application of millet bran active polyphenol in preparation of product for preventing and treating atherosclerosis
  • Application of millet bran active polyphenol in preparation of product for preventing and treating atherosclerosis
  • Application of millet bran active polyphenol in preparation of product for preventing and treating atherosclerosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Preparation method of millet rice bran active polyphenols

[0024] (1) Take 800mL distilled water and 3200mL acetone according to the volume ratio to prepare 80% acetone solution, and pre-cool at -40°C for 12 hours;

[0025] (2) Pulverize millet bran, take 100 g of millet bran powder, add 2000 mL of pre-cooled 80% acetone, and stir evenly at 4°C for 120 min;

[0026] (3) After fully stirring, centrifuge the mixture at 4°C and 11000rpm / min for 10min, take the supernatant and store it at 4°C, add the precipitate to 2000mL of pre-cooled 80% acetone, and stir evenly at 4°C for 120min;

[0027] (4) After fully stirring, centrifuge the mixture for 10 min at 4°C and 11,000 rpm / min, take the supernatant and discard the precipitate;

[0028] (5) Mix the supernatants obtained twice in (3) and (4), and rotate evaporate at 60° C. until the acetone is removed to obtain the extract; recover the acetone evaporated by rotation and reuse;

[0029] (6) The extract is suction...

Embodiment 2

[0031] Example 2: Effects of millet rice bran active polyphenols on the migration of aortic smooth muscle cells

[0032] Take the aortic smooth muscle cells in the logarithmic growth phase and adjust the cell density and inoculate them in a 24-well cell culture plate with a sterilized tip circle. Remove the pipe tip ring from the culture plate, use a sterilized 20 μL pipette tip to scratch evenly in the monolayer cell circle, suck off the old medium, wash three times with PBS, add 500 μL containing 2% serum and 10 -6 The new medium of mol / L angiotensin Ⅱ (Ang Ⅱ) was treated with 10%, 20%, 30% and 40% polyphenol components of the same final concentration (0.15mg / mL) for 24h, and the Take pictures of the scratch distance at 0h and 24h to observe the cell migration. Cell migration rate = (scratch distance of 0h dosing group-scratch distance of 24h dosing group) / (scratch distance of 0h control group-scratch distance of 24h control group)

[0033] The results showed that: compare...

Embodiment 3

[0034] Example 3: Effect of Millet Rice Bran Active Polyphenols on Lipid Phagocytosis by Aortic Smooth Muscle Cells (HASMC)

[0035] Soak the coverslip in acid overnight, rinse it with distilled water, and soak it in 70% alcohol. When using, dry the alcohol on the coverslip and spread it in a 24-well plate. The concentration diluted with serum-free medium is 20000 / mL Aortic smooth muscle cells (HASMC) were plated in 24-well plates and cultured for 24 hours. After the treatment was completed, the serum-free medium of aortic smooth muscle cells (HASMC) was discarded, and 1 mL containing 10 -6 moL / L AngⅡ complete medium. Then add millet rice bran active polyphenols (MSP) at a final concentration of 0.1 mg / mL and 0.15 mg / mL, and treat for 24 hours. Aspirate off the medium, wash twice with 37°C preheated PBS (PH=7.4), add 500 μL ORO Fixative fixative to each well for 30 min, discard the fixative, wash twice with distilled water, add 60% isopropanol to soak Wash for 5 minutes, th...

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Abstract

The invention discloses application of millet bran active polyphenol in preparation of a product for preventing and treating atherosclerosis. Experiments prove that the millet bran active polyphenol can significantly inhibit migration ability of human aortic smooth muscle cells, can significantly inhibit lipid phagocytosis ability of the human aortic smooth muscle cells and human mononuclear macrophages, and can inhibit formation of in-vivo atherosclerotic plaques, thereby preventing and treating atherosclerosis. The millet bran active polyphenol is taken as a main component and can be used for preparing functional foods or medicines for preventing and / or treating atherosclerosis.

Description

technical field [0001] The present invention relates to the fields of functional food and medicine, in particular to the new use of millet rice bran active polyphenols, specifically the application of millet rice bran active polyphenols in the preparation of functional foods or medicines for preventing and / or treating atherosclerosis. Background technique [0002] Millet (scientific name: Setaria italica) belongs to the genus Setaria of the family Poaceae, originated from the Yellow River Basin of my country, and is one of the main special products in North China. Millet is millet after shelling, and the shell that it takes off is millet bran. "Huangdi Neijing" records: the rice bran is sweet, flat in nature, and tends to invigorate qi. Millet rice bran is rich in nutrients and beneficial ingredients. The active polyphenolic compound of millet rice bran is a renewable green resource with rich content, green environmental protection, and no toxic side effects. Studies have f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/105A61K36/899A61P9/10A61K131/00
CPCA23L33/105A61K36/899A61P9/10A23V2002/00A61K2236/333A61K2236/53A61K2236/55A61K2236/51A23V2200/30A23V2250/2132
Inventor 李卓玉刘丰铭单树花李汉卿史江颖
Owner SHANXI UNIV
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