Lys-Glu modified indoloquinolizine, preparation, nano-structure, activity and application thereof

A technology of -lys-glu and quinolizine, which is applied in the field of its anti-tumor cell adhesion, invasion and migration, and the preparation of anti-tumor drugs, can solve the problems of not obtaining anti-tumor and anti-tumor metastatic compounds and the like

Inactive Publication Date: 2016-01-27
CAPITAL UNIVERSITY OF MEDICAL SCIENCES
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the inventor has spent a lot of research effort and screened hundreds of compounds, no compound with both anti-tumor and anti-metastasis effects has been obtained

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lys-Glu modified indoloquinolizine, preparation, nano-structure, activity and application thereof
  • Lys-Glu modified indoloquinolizine, preparation, nano-structure, activity and application thereof
  • Lys-Glu modified indoloquinolizine, preparation, nano-structure, activity and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1 prepares L-tryptophan benzyl phosphate (1)

[0027] Add 20.4g (100mmol) of L-tryptophan, 120mmol of benzyl alcohol and 120mmol of polyphosphoric acid into a 500ml eggplant bottle in turn, install a condenser at the mouth of the bottle, place it in an oil bath and heat to 80°C, react for 72 hours, TL°C shows The starting material disappeared completely, and the reaction was terminated. The reaction solution was cooled to room temperature, and 30 ml of anhydrous ether was added to precipitate a white solid, which was stirred for 2 hours. The reaction solution was filtered under reduced pressure to obtain a white solid, which was washed three times with anhydrous ether to obtain 35.4 g (90.4%) of the target compound. It is a white solid. ESI-MS(m / e): 393.1[M+H] + .

Embodiment 2

[0028] Example 2 Preparation of (3S)-1-(2,2-dimethoxyethyl)-2,3,4,9-tetrahydro-β-carboline-3-carboxylic acid benzyl ester (2)

[0029] 250ml CH 2 Cl 2 Add it to a 500ml eggplant bottle, add 15ml 1,1,3,3-tetramethoxypropane and 14ml trifluoroacetic acid successively under ice bath, stir for 0.5 hours, add 20.0g (68mmol) L-tryptophan benzyl ester, stir at room temperature After 120 hours, the reaction ended. Slowly add saturated Na to the reaction solution with stirring in an ice bath 2 CO 3 aqueous solution, stirred vigorously until the pH of the aqueous layer was 7, separated the dichloromethane layer, and washed the dichloromethane layer with 5% NaHCO 3 aqueous solution and saturated NaCl aqueous solution were extracted and washed 3 times, and the dichloromethane layer was washed with anhydrous NaCl 2 SO 4 Dry, filter under reduced pressure, and concentrate the filtrate to dryness under reduced pressure. The residue is purified by silica gel column chromatography (petro...

Embodiment 3

[0030] Example 3 Preparation of (3S)-1-(2,2-dimethoxyethyl)-2-acetoacetyl-2,3,4,9-tetrahydro-β-carboline-3-carboxylic acid benzyl ester (3)

[0031]Dissolve 9.38g (23.8mmol) (3S)-1-(2,2-dimethoxyethyl)-2,3,4,9-tetrahydro-β-carboline-3-carboxylic acid benzyl ester in 200ml Acetone, 2.89ml of diketene and 1.81ml of triethylamine were added under stirring in an ice bath, and stirred at room temperature for 18 hours. TLC showed that the raw materials completely disappeared, and the reaction was completed. Add 10ml of distilled water under stirring in an ice bath, stir for 1 hour, concentrate under reduced pressure to remove acetone, extract the residue 3 times with dichloromethane, combine the dichloromethane layers, and successively wash with 5% NaHCO 3 aqueous solution and saturated NaCl aqueous solution were extracted and washed 3 times, and the dichloromethane layer was washed with anhydrous NaCl 2 SO 4 After drying and filtering under reduced pressure, the filtrate was con...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses (6S)-3-acetyl-4-oxo-4,6,7,12-tetrahydroindole [2,3-a]quinolizine-6-formyl-Lys-Glu with a structure in the specification. The invention discloses a preparation method of the compound, discloses its nano-structure and anti-tumor effect, discloses an effect of inhibiting adhesion, invasion and migration of tumor cells and expounds an application of the compound in medical science.

Description

technical field [0001] The present invention relates to (6S)-3-acetyl-4-oxo-4,6,7,12-tetrahydroindo[2,3-a]quinazine-6-formyl-Lys-Glu. It relates to its preparation method, its nanostructure, its in vivo and in vitro anti-tumor effects, and its anti-tumor cell adhesion, invasion, and migration effects. Therefore, the present invention relates to its application in the preparation of anti-tumor drugs and anti-tumor cell adhesion, invasion and migration drugs. The invention belongs to the field of biomedicine. technical background [0002] Malignant tumors seriously threaten human health. In addition to the poor prognosis of cancer patients, the inflammation, thrombus and metastasis of malignant tumors further worsen the prognosis of patients. For example, more than 90% of malignant tumor patients died of tumor metastasis. Tumor metastasis depends on four factors: 1) microthrombosis forms on the surface of tumor cells, escapes macrophage phagocytosis, and migrates to the di...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07K5/068C07K1/02B82Y30/00A61K38/05A61P35/00
Inventor 彭师奇赵明王玉记吴建辉周倩羽
Owner CAPITAL UNIVERSITY OF MEDICAL SCIENCES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap