Application of S-propargyl-cysteine to preparation of medicament for treating digestive system tumors

A cysteine ​​and digestive system technology, which is used in the field of propargyl cysteine ​​in the preparation of drugs for treating digestive system tumors

Active Publication Date: 2012-01-11
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The protective effect of SPRC on cardiomyocyte hypoxic injury has been reported, but there is no report on the anti-tumor effect of propargyl cysteine ​​(SPRC), especially in the treatment of digestive system tumor drugs

Method used

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  • Application of S-propargyl-cysteine to preparation of medicament for treating digestive system tumors
  • Application of S-propargyl-cysteine to preparation of medicament for treating digestive system tumors
  • Application of S-propargyl-cysteine to preparation of medicament for treating digestive system tumors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Cell Lines and Culture Mode

[0025] Human gastric cancer cell line (SGC-7901) and human liver cancer cell line (HepG2) (purchased from Cell Bank of Shanghai Institute of Biological Sciences, China). Culture conditions: culture SGC-7901 and HepG2 cells in RPMI 1640 and DMEM medium containing 10% fetal bovine serum and 1-2% penicillin and streptomycin (100U / ml), respectively, and place the cells at 37°C, 5% CO 2 cultured in an incubator.

Embodiment 2

[0026] Embodiment 2SPRC reduces cell viability experiment

[0027] 10 per hole 3 -10 4 SGC and HepG2 cells were seeded in a 96-well plate with a volume of 200ul per well. Add SPRC to final concentration respectively to 10 -5 、10 -6 、10 -7 、10 -8 and 10 -9 mol / L. After 24 hours of treatment, the cell viability was detected by MTT method, and the western medicine control group was treated with 10 -5 mol / L paclitaxel liposomes were processed accordingly and detected by MTT. The result is as figure 1 shown, 10 -5 , 10 -6 , 10 -7 The cell viability of the mol / L SPRC treatment group was significantly lower than that of the normal control group, and SPRC could inhibit the cell viability of liver cancer and gastric cancer cells.

Embodiment 3

[0028] Example 3 SPRC promotes cell apoptosis experiment

[0029] SGC and HepG2 cells were seeded in culture dishes with 10 -5 mol / L SPRC for 24 hours, and with 10 -5 mol / L paclitaxel liposomes were treated accordingly. Hoechst staining results as figure 2 As shown, the observed results showed obvious morphological features of apoptosis such as nuclear condensation and nuclear fragmentation in SPRC-treated SGC and HepG2 cells.

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Abstract

The invention belongs to the field of pharmacy and relates to application of S-propargyl-cysteine (SPRC) to preparation of a medicament for treating digestive system tumors. In the invention, a class of broad-spectrum chemotherapeutic medicament, namely taxol, is adopted as a contrast, and in-vitro gastric cancer and liver cancer cell culture experiments are performed to detect the activities of caner cells. Results show that the SPRC in the invention can be used for decreasing cell survival rate, promoting cell apoptosis, decreasing cell proliferation and migration capabilities, enhancing apoptosis related factors Bax and p53, and reducing the mRNA (Messenger Ribonucleic Acid) and protein expressing level of an anti-apoptotic factor Bcl-2; and the SPRC is demonstrated to play a role in inhibiting the gastric cancer and the liver cancer. The SPRC can be prepared into treatment medicaments to be applied to treatment of the gastric cancer and the liver cancer.

Description

technical field [0001] The invention belongs to the field of pharmacy, and relates to a new drug application of propargyl cysteine ​​(SPRC, S-propargyl-cysteine), in particular to the application of propargyl cysteine ​​(SPRC) in the preparation of drugs for treating digestive system tumors . Background technique [0002] Allyl cysteine ​​(SAC, S-allycysteine) is a natural organosulfur compound in garlic, and is the main active ingredient in mature garlic extract (Aged garlic extract), composed of S-alk(en)yl cystesteine ​​sulfoxides( ACSs) are obtained by degradation. It has been reported in the literature that this compound has antitumor, antibacterial and antifungal properties. [0003] Propargyl cysteine ​​(SPRC, S-propargyl-cysteine) is similar to SAC (S-allycysteine) and has the same cysteine ​​structure. The protective effect of SPRC on cardiomyocyte hypoxic injury has been reported, but there is no report on the anti-tumor effect of propargyl cysteine ​​(SPRC), es...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/198A61P35/00
Inventor 朱依谆卡尤姆
Owner FUDAN UNIV
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