A blood purification modified membrane and its preparation method

A blood purification and modification technology, applied in chemical instruments and methods, blood filtration, membrane technology, etc., can solve the problem of less attention to the anti-inflammatory effect of membrane materials, and achieve good blood compatibility, low cost, and anti-inflammatory properties. Good anti-thrombotic properties

Active Publication Date: 2022-04-12
XIANGYA HOSPITAL CENT SOUTH UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, most of the blood purification membranes are modified only to inhibit the thrombus formation of membrane materials by increasing their hydrophilic properties or anticoagulant properties, but there is no modified membrane that inhibits thrombus formation by targeting and inhibiting the platelet P2Y12 receptor, and currently Less attention has been paid to the anti-inflammatory effect of membrane materials

Method used

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  • A blood purification modified membrane and its preparation method
  • A blood purification modified membrane and its preparation method

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preparation example Construction

[0044] Specifically, the preparation method of a blood purification modified membrane provided by this application includes the following steps:

[0045] (1) Preparation of citric acid-coupled chitosan (CA-CTS): citric acid (CA) and EDC (1-(3-dimethylaminopropyl)-3-ethylcarbodiimide) Dissolve in anhydrous DMSO (dimethyl sulfoxide), stir until dissolved, add CTS (chitosan)-acetic acid-sodium acetate buffer, stir for 12-48 hours to obtain a mixed solution; adjust the concentration of the mixed solution with NaOH pH to 8.0-9.0, filter, and take the precipitate to obtain citric acid-coupled chitosan (CA-CTS); the mass ratio of the citric acid to the EDC is (100-150): (15-20) , each milliliter of the DMSO contains 6.67-10 mg of citric acid;

[0046] (2) Preparation of CA-CTS-loaded ticagrelor microspheres: Dissolve the citric acid-coupled chitosan (CA-CTS) in step (1) in acetic acid solution, then add ticagrelor, and stir well , to obtain a mixed solution; dropwise add liquid par...

Embodiment 1

[0069] (1) Preparation of citric acid-coupled chitosan (CA-CTS)

[0070] Dissolve 150 mg of citric acid and 20 mg of EDC in 15 ml of anhydrous DMSO, stir magnetically at room temperature and protect from light until completely dissolved, then add 50 ml of 5 mg / ml CTS acetic acid-sodium acetate buffer (pH5.0), stir magnetically at room temperature and protect from light for 24 hours, The mixed solution was obtained; the pH of the mixed solution was adjusted to 9.0 with NaOH, filtered, the precipitate was taken, washed 3 times with distilled water, and vacuum-dried for 24 hours to obtain citric acid-coupled chitosan (CA-CTS);

[0071] (2) Preparation of CA-CTS loaded ticagrelor microspheres (prepared by emulsification cross-linking method)

[0072] Dissolve 90 mg of CA-CTS in step (1) in 5 ml of 2% acetic acid solution, then add 30 mg of ticagrelor and stir evenly to obtain a mixed solution; slowly drop into the mixed solution containing 6 g of Span 80 120ml of liquid paraffin,...

Embodiment 2

[0076] (1) Preparation of citric acid-coupled chitosan (CA-CTS)

[0077] Dissolve 100 mg of citric acid and 15 mg of EDC in 15 ml of anhydrous DMSO, stir magnetically at room temperature and protect from light until completely dissolved, then add 30 ml of 5 mg / ml CTS acetic acid-sodium acetate buffer (pH4.0), stir magnetically at room temperature and protect from light for 12 hours, The mixed solution was obtained; the pH of the mixed solution was adjusted to 8.0 with NaOH, filtered, the precipitate was taken, washed 3 times with distilled water, and dried in vacuum for 24 hours to obtain citric acid-coupled chitosan (CA-CTS);

[0078] (2) Preparation of CA-CTS loaded ticagrelor microspheres (prepared by emulsification cross-linking method)

[0079] Dissolve 60 mg of CA-CTS in step (1) in 5 ml of 2% acetic acid solution, then add 20 mg of ticagrelor and stir evenly to obtain a mixed solution; slowly drop into the mixed solution containing 3 g of Span 80 120ml of liquid paraff...

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Abstract

The invention discloses a blood purification modified membrane and a preparation method thereof. The preparation method comprises the following steps: (1) preparation of citric acid-coupled chitosan; (2) preparation of CA‑CTS-loaded ticagrelor microspheres; and (3) preparation of a blood purification modified membrane. The invention utilizes the hydrophilicity and anticoagulant properties of citric acid to prepare a blood purification modified membrane by putting ticagrelor into the microsphere particles, so that the modified membrane can directly act on the P2Y12 receptor, thereby directly inhibiting Platelet activity, in order to achieve the effect of slow-release anti-inflammatory and anti-thrombotic, and the release is stable. The blood purification modified membrane of the present application has low cost, good anti-inflammatory and antithrombotic properties, can release ticagrelor into the blood stably and slowly, and has good blood compatibility.

Description

technical field [0001] The invention belongs to the technical field of membranes, and in particular relates to a blood purification modified membrane and a preparation method thereof. Background technique [0002] Blood purification therapy based on hemodialysis, artificial liver, and membrane lung has become an important means of treatment for acute and chronic liver, renal failure, acute respiratory distress syndrome, systemic inflammatory response syndrome and other critical illnesses. The membrane material needs to be in direct contact with human blood. If the blood compatibility is not good, it is easy to induce coagulation and thrombus, affect the blood purification effect, and delay the disease. Therefore, anticoagulant and antithrombotic treatment must be routinely performed clinically. Anticoagulant and antithrombotic modification of membrane materials has become a research hotspot and difficulty in the field of blood purification membrane materials. At the same ti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D67/00B01D69/12B01D69/02A61M1/16C08B37/08A61M1/34
CPCB01D67/0011B01D67/0013B01D67/0016B01D69/12B01D69/02B01D67/0093C08B37/00A61M1/34B01D71/68B01D71/48B01D71/42B01D71/50B01D71/72
Inventor 府晓雷霆宁建平彭捷陈聪汤赐
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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