Biomarker for diagnosing mental diseases and application thereof
A technique for biomarkers, psychiatric diseases, applied in the field of molecular diagnosis
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Embodiment 1
[0076] Screening and Modeling of Biomarkers for Diagnosis of Psychiatric Disorders
[0077] (1) Peripheral venous blood was collected from patients with first-episode mental illness (748 cases in total, including 156 cases of schizophrenia, 228 cases of bipolar disorder, and 364 cases of depression) and healthy controls (263 cases), extracted plasma, and placed in Store at -80°C for later use;
[0078] Take 90 microliters of plasma, add 10 microliters of internal standard solution, then add 400 microliters of extraction solution, vortex for 30 seconds, sonicate for 5 minutes, centrifuge at 13000rpm for 10 minutes, and take the supernatant to obtain the sample to be tested;
[0079] Preparation of internal standard solution: Accurately weigh L-2-chlorophenylalanine, dissolve it in methanol, and prepare a 10 μg / mL solution;
[0080] Preparation of extraction solution: mix chromatographic grade dichloromethane and methanol according to the volume ratio of 2:1;
[0081] Quality ...
experiment example 1
[0089] (1) Evaluate the diagnostic effect of the predictive model that embodiment 1 establishes to mental illness
[0090] Randomly take blood from outpatients or inpatients in a certain hospital, and blood from recruited healthy controls (a total of 475 samples, all samples have complete scale data, and signed informed consent), and refer to the method of Example 1 Direct detection of ion abundance ratios of biomarkers (tryptophan, ornithine, creatinine, carnosine, L-carnitine, isoleucine, phenylalanine, alanine, and glutamate) to internal standards Input the predictive model that embodiment 1 establishes, obtain diagnostic result, this diagnostic result and this 475 examples of sample by professional doctor carry out the result of mental disease (comprising schizophrenia, bipolar affective disorder and depression) diagnosis by questionnaire scale For comparison, the results are shown in Table 1 and figure 2 ;
[0091] Table 1
[0092]
[0093] It can be seen from Tabl...
Embodiment 2
[0097] Screening and Modeling of Biomarkers for Distinguishing Bipolar Disorder, Depression, and Schizophrenia.
[0098] Method: use the LC-MS / MS detection data of three kinds of psychiatric patients of embodiment 1 (156 cases of schizophrenia, 228 cases of bipolar disorder and 364 cases of depression), refer to the PLS-DA multivariate analysis of embodiment 1 method, screened for combinations of markers capable of distinguishing three psychiatric disorders: bipolar disorder, depression, and schizophrenia (see Figure 4 ): tryptophan, ornithine, creatinine, carnosine, L-carnitine, isoleucine, phenylalanine, threonine and cystine, and use support vector machine (SVM) to establish a prediction model for Differentiate between bipolar disorder, depression, and schizophrenia.
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