Application of CGP 57380 in preparation of medicine for preventing or treating type 2 diabetes
A type 2 diabetes and drug technology, applied in the field of biomedicine, can solve the problems that the research on metabolic diseases has not been reported yet
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0027] Example 1 Experimental verification that CGP 57380 can be used as an inhibitor of the kinase BRSK2, and its enzyme activity is detected at the same time
[0028] The following experiments were entrusted to Shanghai Ruizhi Chemical Research Co., Ltd. to complete.
[0029] 1. Preparation of experimental materials:
[0030] 1) BRSK2 (Carna, Cat.No 02-116, Lot.No 08CBS-0302);
[0031] 2) Peptide10 (invitrogen, Cat. No 116583 Lot. No P130913-XP116583);
[0032] 3) ADP-Glo TM Kinase Assay (Promege, Cat.No v9102 / 3, Lot.No 314795);
[0033] 4) ATP (Sigma, Cat. No. A7699-1G, CAS No. 987-65-5);
[0034] 5) DMSO (Sigma, Cat. No. D2650, Lot. No. 474382);
[0035] 6) EDTA (Gibco, Cat. No. 15575, Lot No. 846901);
[0036] 7) 96-well plate (Corning, Cat.No.3365, Lot.No.22008026);
[0037]8) 384-well plate (Corning, Cat.No.4512, Lot.No.13315417);
[0038] 9) Staurosporine (Sellekchem, Cat. No. S1421, Lot. No. S142105).
[0039] 2. Kinase response system:
[0040] 1)1xkinase b...
Embodiment 2
[0068] Example 2 The effect of CGP 57380 on BRSK2 activity and protein level was detected at the cell level of MIN6, and the effect of CGP 57380 on the glucose-stimulated insulin secretion function was detected at the cell level and on primary pancreatic islets.
[0069] Cell line: mouse islet β cell line MIN6 ( DOI: 10.1210 / endo-127-1-126 ), human primary islets (Tianjin First Central Hospital).
[0070] Experimental drug: CGP 57380 was purchased from MCE Company, and the negative control was DMSO.
[0071] The specific steps are: 20-24 hours after the MIN6 cells were plated, the cells were treated with CGP 57380 (10uM) for 12 hours and 24 hours respectively, the control group was set as DMSO treatment, and Western Blot was used to detect the phosphorylation level of the BRSK2 substrate and the protein level of BRSK2 in the cells The change. Under the same treatment conditions, GSIS detects the function of the cells. The detailed steps are to pre-equilibrate MIN6 cells wit...
Embodiment 3
[0077] Example 3 Effect of CGP 57380 on metabolic phenotype of type 2 diabetic mice.
[0078] Experimental animals: C57BL / 6J mice of 3-4 weeks were fed with normal diet (NCD) and high-fat diet (HFD) for about 4 months, and the animals were randomly divided into cages and fed freely.
[0079] Experimental steps:
[0080] 1) Mice fed with normal feeding and high-fat feeding for about 4 months were randomly divided into 5-7 mice in each group. The HFD group was given CGP 57380 (HFD CGP 57380) and the negative control (HFD Vehicle) respectively, and the NCD group Only the negative control (NCDVehicle) was administered intragastrically, once a day, at a dose of 10 mg / kg;
[0081] 2) The blood sugar and body weight of the mice were detected every week for 4-5 weeks. The blood sugar measurement method was: take 1 drop of tail vein blood, and measure random blood sugar with blood sugar test strips and a rapid blood glucose meter;
[0082] 3) The level of glucose tolerance of the mic...
PUM
Login to view more Abstract
Description
Claims
Application Information
Login to view more - R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap