Single-leaf grafting inoculation method for tomato yellow leaf curl virus and tomato chlorosis virus

Abstract

The invention discloses a single-leaf grafting inoculation method for tomato yellow leaf curl virus and tomato chlorosis virus, which comprises the following steps: S1, rootstock treatment: taking tomato seedlings or lateral branches, selecting proper nodes, removing healthy leaves from the nodes, and making oblique cuts on stems of receptor seedlings; S2, scion treatment: taking a single leaf with good growth vigor as a scion material, and cutting a petiole inoculated with the single leaf into a wedge shape; S3, cuttage grafting: obliquely inserting the scions into the cuts of the rootstocks, fixing the scions with grafting clamps, and culturing the scions. The single-leaf grafting inoculation method for tomato yellow leaf curl virus and tomato chlorosis virus is not affected by virus transmission mediators and is not limited by seasons, grafting materials are easy to obtain, and operation is easy and convenient.

Description

technical field [0001] The invention relates to the technical field of plant protection, in particular to a method for single-leaf grafting and inoculation of tomato yellow leaf curl virus and tomato chlorosis virus. Background technique [0002] Tomato yellow leaf curl virus (Tomato yellow leaf curl virus) and tomato chlorosis virus (TomatoChlorosis virus) are two worldwide tomato virus diseases transmitted by Bemisia tabaci. They occur and distribute widely in my country, seriously endangering tomato in my country. Production. At present, the application of chemical agents to control the virus-transmitting vector Bemisia tabaci is the main control method for this type of virus disease, but Bemisia tabaci is very easy to develop drug resistance, chemical control is likely to cause environmental and food pollution, and reduce the quality of agricultural products. Disease species is the most fundamental and effective measure. [0003] The research on the resistance of tomato...

AI Technical Summary

Problems solved by technology

In production and scientific research, there are many reports on the use of whitefly and infectious clones for virus inoculation, but the use of whitefly inoculation needs to obtain whitefly populations carrying different viruses, because whitefly simultaneously transmits tomato yellowing. For leaf curl virus and tomato chlorotic virus, it is difficult to obtain whitefly populations carrying different viruses, the inoculation conditions are not easy to control, and the accuracy of virus inoculation is limited in the study of virus-host interaction; the invasive clone of virus It can achieve timing and quantitative inoculation of viruses, but it is difficult to prepare, develop, obtain, and costly, making it difficult to apply on a large scale

In addition, there are few reports on virus grafting and inoculation techniques. Only Yu Li et al. reported that the grafting technique was used to achieve the grafting and inoculation of tomato yellow leaf curl virus. However, this method uses diseased tomato materials as rootstocks and healthy tomato materials as scion When grafting, only two true leaves of the rootstock material are kept, and the seedling age of the rootstock and the poisonous source need to be consistent. The rootstock material used as the poisonous source can only be used once. Repeated inoculation requires a large number of poisonous sources to be propagated, which consumes a lot of manpower and material resources

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