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A golden ear ginseng fermentation extract with antioxidant capacity

A technology of anti-oxidation ability and extraction solution, which is applied in the field of fermented extraction solution of golden ear ginseng

Active Publication Date: 2022-03-04
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Researcher Deng Chao and others invented a method for preparing polysaccharides from Auricularia aureus fermentation broth with antioxidant activity, but this method focused on the preparation of Auricularia polysaccharides and did not mention other biologically active substances

Method used

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  • A golden ear ginseng fermentation extract with antioxidant capacity
  • A golden ear ginseng fermentation extract with antioxidant capacity
  • A golden ear ginseng fermentation extract with antioxidant capacity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Preparation of primary seed liquid: take golden ear as the fermentation strain, pick 5 pieces of soybean-sized bacterium block and add 50mL PDA liquid medium as seed liquid medium, inoculum size 5% (v / v), 24°C , 160r / min cultured for 5 days, and obtained the first-class seed liquid.

[0037] (2) Preparation of the secondary seed liquid: the primary seed liquid in step (1) is used as the fermentation strain to prepare the secondary seed liquid, and the preparation method is the same as the preparation method of the primary seed liquid in step (1).

[0038] (3) solid-state fermentation: the secondary seed liquid of step (2) is inoculated in the 15g solid-state fermentation medium by the inoculum size of 15% (v / v), and the fermentation substrate of solid-state fermentation medium is bran, soybean hulls and The mass ratio of corn bran, bran, soybean hulls and corn bran is 1:1:1, and the feeding amount of ginseng extract is 0.05g / g fermentation substrate. Under the cond...

Embodiment 2

[0041] (1) Preparation of first-level seed liquid: take golden ear as the fermentation strain, pick 5 soybean-sized bacterium blocks and add 50mL PDA liquid medium to be the seed liquid medium, inoculum size 20% (v / v), 28°C , 200r / min cultured for 7 days to obtain a first-class seed liquid.

[0042] (2) Preparation of the secondary seed liquid: the primary seed liquid in step (1) is used as the fermentation strain to prepare the secondary seed liquid, and the preparation method is the same as the preparation method of the primary seed liquid in step (1).

[0043] (3) solid-state fermentation: the secondary seed liquid of step (2) is inoculated in the 25g solid-state fermentation medium by the inoculum size of 25% (v / v), and the fermentation substrate of solid-state fermentation medium is bran, soybean hulls and Corn bran, bran, soybean bran and corn bran mass ratio is 1:1:1, the feeding amount of ginseng extract is 0.2g / g fermentation substrate, under the conditions of 28 ℃, i...

Embodiment 3

[0046] (1) Preparation of first-level seed liquid: take golden ear as the fermentation strain, pick 5 soybean-sized bacterium blocks and add 50mL PDA liquid medium as seed liquid medium, inoculum size 10% (v / v), 25°C , 180r / min cultured for 7 days to obtain a first-class seed liquid.

[0047] (2) Preparation of the secondary seed liquid: the primary seed liquid in step (1) is used as the fermentation strain to prepare the secondary seed liquid, and the preparation method is the same as the preparation method of the primary seed liquid in step (1).

[0048] (3) solid-state fermentation: the secondary seed liquid of step (2) is inoculated in the 20g solid-state fermentation medium by the inoculum size of 20% (v / v), and the fermentation substrate of solid-state fermentation medium is bran, soybean hulls and The mass ratio of corn bran, soybean bran and corn bran is 1:1:1, the feeding amount of ginseng extract is 0.1g / g fermentation substrate, and the static culture is carried out...

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Abstract

The invention discloses a golden ear ginseng fermentation extraction liquid with antioxidant capacity. Use golden ear strains as fermentation strains, inoculate the seed liquid into the culture medium with agricultural products as the fermentation substrate and ginseng extract, solid-state fermentation culture, and obtain products rich in biologically active substances after fermentation, and use purified water to ferment the products Extraction is carried out, and an extract solution with antioxidant capacity is obtained after heating and homogenizing (Figure 1). The golden ear ginseng fermentation extract prepared by the method of the present invention has antioxidant properties, the total antioxidant capacity is 2.06mmolTE / L, the catalase activity is 7.11U / mL, and the ability to inhibit hydroxyl radicals is 107.32U / mL. The oxygen free radical scavenging ability is 5383.31μmol / L. The golden ear ginseng fermented extract prepared by the method of the invention shows good antioxidant capacity, and can be applied to prepare health care products with antioxidant capacity and anti-aging functions.

Description

technical field [0001] The invention relates to a golden ear ginseng fermentation extract with anti-oxidation ability, and belongs to the technical field of microbial fermentation. Background technique [0002] Tremella aurantialba (Tremella aurantialba) is a colloidal edible and medicinal fungus of the genus Tremella. Due to its excellent nutritional value and biological activity, it is a rare edible and medicinal fungus in my country, and is often used as traditional medicine and food. Studies have shown that the fermented liquid of golden ear contains essential amino acids for the human body, and the polysaccharide obtained by hot water extraction has certain anti-oxidation and hypoglycemic effects, and is a nutritious and green health food. Ginseng is a precious plant, known as the "king of traditional Chinese medicine". Ginsenosides are the main medicinal components of ginseng with various pharmacological and biological activities. Rare ginsenosides have stronger biol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/9789A61K8/9728A61Q19/08A23L33/00
CPCA61K8/9789A61K8/9728A61Q19/08A23L33/00A23V2002/00A61K2800/85A61K2800/5922A23V2200/30
Inventor 余晓斌杨文华顾秋亚周剑丽张将
Owner JIANGNAN UNIV