Method for identifying Maotai-flavor Baijiu of specific brand by adopting nuclear magnetic resonance and partial least square method based on non-volatile substances
A partial least squares and nuclear magnetic resonance technology, applied in the field of Maotai-flavor liquor identification, can solve problems such as error in identification results, cumbersome experimental operation, taste, taste, and health function effects, and achieve excellent application effects.
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[0036] The following is further described in detail through specific implementation methods:
[0037] 1. Instruments, reagents and samples
[0038] 1.1 Instruments: NMR spectrometer (Bruker, 600MHz), SIMCA software (Umetrics), centrifuge (Eppendorf, Germany), NMR tube
[0039] 1.2 Reagents: phosphoric acid (≥85%, aladdin), sodium dihydrogen phosphate (≥99.0%, Macklin), 3-(trimethylsilyl) deuterated sodium propionate (98%, Sigma-Aldrich), heavy water (US CIL)
[0040]1.3 Samples: A total of 33 samples of finished Maotai-flavored Maotai-flavored liquors: including 7 specific brands of Maotai-flavored liquors (numbered GB1~GB7), 15 other brands of Maotai-flavored liquors (numbered QT1~QT15), and 11 unknown liquors Samples (numbered YZ1-YZ11), the design rules for 11 unknown wine samples are: one is a specific brand of Maotai-flavored liquor, and the rest are other brands of Maotai-flavored liquor.
[0041] 2. Wine sample pretreatment
[0042] Precisely draw 5mL Maotai-flavore...
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