Method for identifying Maotai-flavor Baijiu of specific brand by adopting nuclear magnetic resonance and partial least square method based on non-volatile substances

A partial least squares and nuclear magnetic resonance technology, applied in the field of Maotai-flavor liquor identification, can solve problems such as error in identification results, cumbersome experimental operation, taste, taste, and health function effects, and achieve excellent application effects.

Inactive Publication Date: 2021-09-17
贵州国台酒业集团股份有限公司
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  • Summary
  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although the non-volatile substances in Maotai-flavored liquor usually do not show aroma, they have a great impact on taste, taste, health functions, etc.
However, the existing identification methods for the authenticity of Maotai-flavored liquor have the following deficiencies: (1) There is no research or application on the authenticity identification of Maotai-flavored liquor specifically for the non-volatile substances of Maotai-flavored liquor
(2) The existing detection methods for non-volatile substances in Maotai-flavored liquor are all targeted detection of a certain type of non-volatile substances, and the detection of non-targeted non-volatile substances cannot be realized
(3) The existing detection methods for non-volatile substances in Maotai-flavored liquor usually require derivatization treatment. This step not only damages the sample and causes errors in the identification results, but also the experimental process is cumbersome.
To sum up, there is no 'non-targeted Maotai-flavored liquor identification method based on the non-volatile substances of Maotai-flavored liquor'

Method used

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  • Method for identifying Maotai-flavor Baijiu of specific brand by adopting nuclear magnetic resonance and partial least square method based on non-volatile substances
  • Method for identifying Maotai-flavor Baijiu of specific brand by adopting nuclear magnetic resonance and partial least square method based on non-volatile substances
  • Method for identifying Maotai-flavor Baijiu of specific brand by adopting nuclear magnetic resonance and partial least square method based on non-volatile substances

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Embodiment Construction

[0036] The following is further described in detail through specific implementation methods:

[0037] 1. Instruments, reagents and samples

[0038] 1.1 Instruments: NMR spectrometer (Bruker, 600MHz), SIMCA software (Umetrics), centrifuge (Eppendorf, Germany), NMR tube

[0039] 1.2 Reagents: phosphoric acid (≥85%, aladdin), sodium dihydrogen phosphate (≥99.0%, Macklin), 3-(trimethylsilyl) deuterated sodium propionate (98%, Sigma-Aldrich), heavy water (US CIL)

[0040]1.3 Samples: A total of 33 samples of finished Maotai-flavored Maotai-flavored liquors: including 7 specific brands of Maotai-flavored liquors (numbered GB1~GB7), 15 other brands of Maotai-flavored liquors (numbered QT1~QT15), and 11 unknown liquors Samples (numbered YZ1-YZ11), the design rules for 11 unknown wine samples are: one is a specific brand of Maotai-flavored liquor, and the rest are other brands of Maotai-flavored liquor.

[0041] 2. Wine sample pretreatment

[0042] Precisely draw 5mL Maotai-flavore...

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Abstract

The scheme discloses the technical field of Maotai-flavor Baijiu identification, and a method for identifying a Maotai-flavor Baijiu of a specific brand by combining a nuclear magnetic resonance hydrogen spectrum (<1>H NMR) technology with a partial least square analysis method based on non-volatile substances. The method comprises:respectively establishing a data set of a Maotai-flavor Baijiu of a specific brand and a data set of Maotai-flavor Baijius of other brands, constructing an identification model, carrying out reliability verification on the identification model, and finally identifying an unknown Baijiu sample to be detected. The method is used for discriminating Maotai-flavor Baijiu of a specific brand and other brands, whether the detected Maotai-flavor Baijiu is the Maotai-flavor Baijiu of the specific brand or not is highly truly judged, risks are avoided to the maximum extent, and economic losses of enterprises with the Baijiu of the specific brand are reduced.

Description

technical field [0001] The invention belongs to the technical field of identification of Maotai-flavored liquor, in particular to the use of hydrogen nuclear magnetic resonance spectrum (NMR) based on the non-volatile matter of Maotai-flavored liquor. 1 H NMR) technology combined with partial least squares analysis to identify whether an unknown wine sample is a specific brand of Maotai-flavored liquor. Background technique [0002] In recent years, the consumption of Maotai-flavored Baijiu (referred to as Maotai Wine) has been booming all over the country. The production process and process of counterfeit wine are completely below the standard of authentic Maotai-flavored liquor, and the production process is unqualified. Although the produced Maotai-flavored liquor is only slightly different in taste, the harm to the body is hard to estimate . At the same time, for a specific brand of Maotai wine, the behavior of confusing the real one in the market will seriously damage...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N24/08G06F17/11G06F17/16
CPCG01N24/08G06F17/11G06F17/16
Inventor 王丽卢君王凡山其木格唐平毕荣宇李长文
Owner 贵州国台酒业集团股份有限公司
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