Method for evaluating oxidation effect of pulmonary surfactant

An evaluation method and technology of active agent, which can be used in measurement devices, instruments, scientific instruments, etc., can solve the problems of high consumption and low efficiency, and achieve the effects of high accuracy, high throughput, and simple and convenient methods.

Active Publication Date: 2021-09-24
SUZHOU UNIV
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  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to solve the above-mentioned technical problems, the present invention provides a kind of evaluation method to pulmonary surfactant oxidation effect, take unsaturated phospholipid as evaluation object, overcome existing The problem of large consumption of research objects and low efficiency in the technology can realize the in vitro evaluation of the oxidation effect of pulmonary surfactant, which has the advantages of rapidity, high throughput, high accuracy and sensitivity

Method used

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  • Method for evaluating oxidation effect of pulmonary surfactant
  • Method for evaluating oxidation effect of pulmonary surfactant
  • Method for evaluating oxidation effect of pulmonary surfactant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) The collected two-dimensional nanomaterials (MoS 2 , GO) were diluted with PBS to different final concentrations (0, 3.12, 6.25, 12.5 and 25 μg / mL), and 500 μL of each concentration was taken for use.

[0036] (2) Weigh 250 μg of unsaturated phospholipid standard PC (18:2), dissolve it in 0.5 mL of chloroform, and add it to a 5 mL glass test tube. The chloroform was blown dry with nitrogen gas, so that the phospholipids formed a uniform film on the glass tube wall. Add different concentrations of nanomaterials into glass test tubes, and set up a blank control group (add 0.5 mL of chloroform containing 250 μg of unsaturated phospholipids, dry the chloroform with nitrogen, and then add 500 μL of PBS), and set 3 parallel samples for each group. . After the nitrogen blowing apparatus was preheated to 37° C., each glass test tube was transferred to the nitrogen blowing apparatus, and oxygen was introduced into the reaction liquid, and the reaction lasted for 2 hours. ...

Embodiment 2

[0039] The collected three-dimensional nanomaterials (Y 2 o 3 、TiO 2 ) were diluted with PBS to different final concentrations (0, 3.12, 6.25, 12.5 and 25 μg / mL), and 500 μL of each concentration was taken for use. Other steps are with embodiment 1. The result is as image 3 shown.

Embodiment 3

[0041] The collected PM2.5 was diluted with PBS to different final concentrations (0, 12.5, 25, 50 and 100 μg / mL), and 500 μL of each concentration was used for later use. Other steps are with embodiment 1. The result is as Figure 4 shown.

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Abstract

The invention relates to a pulmonary surfactant oxidation effect evaluation method, which comprises: co-incubating an unsaturated phospholipid monomolecular layer and an inhalable substance, allowing the hydrophobic side of the unsaturated phospholipid monomolecular layer to be close to the inhalable substance and the hydrophilic side of the unsaturated phospholipid monomolecular layer to be far away from the inhalable substance, after the inhalable substances are removed, detecting the lipid peroxide, and judging the oxidation degree of the pulmonary surfactant according to the content of the lipid peroxide. The method overcomes the problems of large consumption of research objects and low efficiency in the prior art, realizes in-vitro evaluation of the oxidation effect of the pulmonary surfactant, and has the advantages of rapidness, high throughput, high accuracy and high sensitivity.

Description

technical field [0001] The invention relates to the technical field of respiratory exposure toxicity, in particular to a method for evaluating the oxidation effect of pulmonary surfactants. Background technique [0002] Pulmonary surfactant, referred to as lung surface activity, is a surface-active lipid and protein complex formed by alveoli. In lung tissue, lipids and proteins form surface-active substances with both hydrophilic and hydrophobic ends. In order to reduce the surface tension of the alveolar fluid-air interface, the hydrophilic group faces the liquid side in the lung, and the hydrophobic group faces the air. side. The main components of lung surface activity are lipids, including saturated lipids and unsaturated lipids. Lung surface activity is also the first barrier for pollutants to enter the lung tissue through the respiratory system. [0003] In recent years, my country has been deeply plagued by haze weather. The main cause of haze weather is the existe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/00
CPCG01N33/00
Inventor 谢仟仟蔡晓明李瑞宾
Owner SUZHOU UNIV
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