Preparation method of detection electrode for pathogenic bacteria in water environment of construction site

A pathogenic bacteria detection and construction site technology, applied in the field of biosensors, can solve the problems of low detection efficiency, inaccurate identification, imperfect preparation process, short service life of electrodes, etc., and achieve fast electron transfer rate, high yield and good stability Effect

Pending Publication Date: 2021-10-01
CHINA MCC17 GRP
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Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of short service life of the electrode, low detection efficiency and inability to accurately identify different types of bacteria due to the imperfect preparation process of the electrode for pathogenic bacteria detection in the water environment in the prior art. Preparation method of electrode for detecting pathogenic bacteria in environment

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  • Preparation method of detection electrode for pathogenic bacteria in water environment of construction site
  • Preparation method of detection electrode for pathogenic bacteria in water environment of construction site
  • Preparation method of detection electrode for pathogenic bacteria in water environment of construction site

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[0022] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them.

[0023] A method for preparing an electrode for detecting pathogenic bacteria in the water environment of a construction site, comprising the following steps:

[0024] S1. Preparation of gold electrodes: photoetching gold interdigitated electrodes on the Si / SiO2 substrate; the shape and structure of which are 20 200 μm long, 5 μm wide and 3 μm between interdigital fingers.

[0025] S2. Electrode surface treatment: The electrode obtained in S1 was subjected to ultrasonic treatment in acetone for 10 minutes, nitrogen gas for 30 minutes, oxygen plasma bath for 10 minutes, and ultraviolet ozone for 30 minutes. (APTES) treated surface;

[0026] S3. Single-wa...

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Abstract

The invention discloses a preparation method of a detection electrode for pathogenic bacteria in the water environment of a construction site. The preparation method comprises the following steps of: S1, preparing a gold electrode; S2, performing electrode surface treatment; S3, modifying on the surface of the electrode with single-walled carbon nanotubes (SWNTs); S4, incubating connecting molecules; and S5, performing a blocking reaction. The electrode for detecting the pathogenic bacteria in the water environment, prepared by the process, is long in service life, high in detection efficiency and capable of accurately identifying different types of bacteria.

Description

technical field [0001] The invention relates to the technical field of biosensors, in particular to a method for preparing a pathogenic bacteria detection electrode in the water environment of a construction site. Background technique [0002] Bacteria detection is of great significance to human health, and the current methods for detecting pathogenic bacteria mainly use antibodies or DNA. Standard methods are enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR). These methods have high sensitivity and specificity. However, they require well-trained personnel and long analysis times. These limitations hinder their use as real-time, wide-range detection platforms, especially when operating in remote outdoor construction sites, where local water sources need to be detected and treated. Simplicity and speed of effective detection technology is the current goal. . [0003] The electrochemical method obtains the map data of different bacteria through ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/48
CPCG01N27/308G01N27/48
Inventor 虞文明王稷汪亚伟倪铭列
Owner CHINA MCC17 GRP
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