Whole-drug-grade raw material tumor tissue cryopreservation liquid and application thereof

A technology of tumor tissue and cryopreservation solution, which is applied in application, preservation of human or animal bodies, animal husbandry, etc., can solve the constraints of industrial transformation and clinical application of live tumor tissue cryopreservation solution, and the inability to evaluate the relative relationship between tissue and fresh tissue Vitality, the relative vitality of the organization has not been fully investigated, and the effect of rich types, good integrity, and wide application range has been achieved.

Active Publication Date: 2021-10-08
SHANGHAI CELL THERAPY GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the latest vitrification cryopreservation technology, CN106047812B disclosure and widely used vitrified tumor tissue cryopreservation solution in the market all have the following deficiencies: (1) complicated and time-consuming operation
(2) High cost
None of them comprehensively investigated the relative viability of the tissue, and could only prove the existence of living cells in the tumor tissue after recovery, but could not evaluate the relative viability of the recovered tissue and fresh tissue under the same volume
The lack of compliant quality research seriously restricts the industrial transformation and clinical application of cryopreserved liquid of human living tumor tissue

Method used

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  • Whole-drug-grade raw material tumor tissue cryopreservation liquid and application thereof
  • Whole-drug-grade raw material tumor tissue cryopreservation liquid and application thereof
  • Whole-drug-grade raw material tumor tissue cryopreservation liquid and application thereof

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specific Embodiment approach

[0051] Below in conjunction with specific embodiment, further illustrate the present invention. The technical solution of the present invention is not limited to the specific embodiments listed below, but also includes any combination of identifications of the specific embodiments.

[0052] tissue cryopreservation solution

[0053] A human living tumor tissue cryopreservation solution (tissue cryopreservation solution for short) is disclosed here, and the tissue cryopreservation solution includes cryoprotectant, human albumin injection and compound electrolyte injection.

[0054] The cryoprotectant can be selected from any one or more of dimethyl sulfoxide, ethylene glycol, polyethylene glycol, polyvinylpyrrolidone, sucrose and trehalose.

[0055] In some embodiments, the concentration of dimethyl sulfoxide in the tissue cryopreservation solution is 0-15% v / v, preferably 1-15% v / v, more preferably 8-12% v / v.

[0056] In some embodiments, the concentration of ethylene glyco...

Embodiment 1

[0180] Embodiment 1 tumor tissue cryopreservation liquid

[0181] A tumor tissue cryopreservation solution, the tissue cryopreservation solution is composed of dimethyl sulfoxide, ethylene glycol, polyethylene glycol, polyvinylpyrrolidone, sucrose, trehalose, human albumin injection and compound electrolyte injection , the concentration of each component is: dimethyl sulfoxide 1% v / v, ethylene glycol 1% v / v, polyethylene glycol 1% w / v, polyvinylpyrrolidone 0.1% w / v, sucrose 1% %w / v, trehalose 1%w / v, human albumin injection 10%v / v, compound electrolyte injection 88%v / v.

Embodiment 2

[0182] Embodiment 2 Tumor tissue cryopreservation solution

[0183]A tumor tissue cryopreservation solution, the tissue cryopreservation solution is composed of dimethyl sulfoxide, ethylene glycol, polyethylene glycol, polyvinylpyrrolidone, sucrose, trehalose, human albumin injection and compound electrolyte injection , the concentration of each component is: dimethyl sulfoxide 15% v / v, ethylene glycol 12% v / v, polyethylene glycol 8% w / v, polyvinylpyrrolidone 5% w / v, sucrose 15% %w / v, trehalose 15%w / v, human albumin injection 30%v / v, compound electrolyte injection 43%v / v.

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Abstract

The invention provides a whole-drug-grade raw material tumor tissue cryopreservation liquid and application thereof, and the tissue cryopreservation liquid comprises the following components: a cryoprotectant, a human albumin injection and a compound electrolyte injection, wherein the cryoprotectant is selected from any one or more of dimethyl sulfoxide, ethylene glycol, polyethylene glycol, polyvinylpyrrolidone, sucrose and trehalose. The invention also provides a method for cryopreserving tumor tissues by using the tissue cryopreservation liquid, and application of the tissue cryopreservation liquid in cryopreservation of human tumor tissues and preparation of human tumor tissue cryopreservation products and the like. By using the tissue cryopreservation liquid, the tumor tissue can be stably cryopreserved for 12 months under the liquid nitrogen condition, the relative activity after resuscitation is high, the cell viability in the tissue is high, the number is large, the types are rich, and the integrity of nucleic acid and protein in the tissue is good.

Description

technical field [0001] The invention relates to the technical field of organization storage methods. More specifically, it relates to live tumor tissue cryopreservation solution and its application. Background technique [0002] According to data from the International Agency for Research on Cancer (IARC) of the World Health Organization, there will be 19.29 million new cancer cases worldwide in 2020, and 9.96 million cancer deaths worldwide in 2020. Cancer has become the number one threat to human health. Tumor tissue is of great significance to the formulation of cancer diagnosis and treatment plans, the discovery of targets and targeted drugs to improve the efficacy of cancer treatment, and the study of tumor pathogenesis. It is one of the core driving forces to promote the development of personalized medicine. [0003] Fresh living tumor tissue has the characteristics of atypia and heterogeneity, completely retains the histological information, cytological information a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 王美霞佟曼郑律红王娟丽师传胤向可欣钱其军
Owner SHANGHAI CELL THERAPY GRP CO LTD
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