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Intelligent high-speed scanning method and device for spot scanning long-life fluorescence microscopic imaging

A high-speed scanning and microscopic imaging technology, applied in the field of optical microscopy, can solve problems such as unclearness, image distortion, and inability to achieve high-speed imaging, and achieve the effect of solving the smearing phenomenon and reducing the scanning speed.

Active Publication Date: 2021-10-15
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, high-speed scanning and imaging of long-lived probes will cause images to be deformed, unclear, and irregular, and the required structural information cannot be intuitively obtained, let alone high-speed imaging.

Method used

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  • Intelligent high-speed scanning method and device for spot scanning long-life fluorescence microscopic imaging
  • Intelligent high-speed scanning method and device for spot scanning long-life fluorescence microscopic imaging
  • Intelligent high-speed scanning method and device for spot scanning long-life fluorescence microscopic imaging

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Embodiment 1

[0033] This embodiment provides an intelligent high-speed scanning device for point-scanning long-life fluorescence microscopy imaging, such as figure 1 As shown, it includes excitation light module, intelligent high-speed scanning module and detection module.

[0034] The excitation light module sequentially includes an excitation light source 1, a half-wave plate 2, a linear polarizer 3, a Gaussian beam expander collimator lens group 4, and a first adjustable mirror 5 along the light propagation direction; The excitation light emitted by the light source 1 is a Gaussian beam. The excitation light reaches the linear polarizer 3 after the power is adjusted by the half-wave plate 2. After passing through the linear polarizer 3, it becomes linearly polarized light and then passes through the Gaussian beam expander collimating lens group. 4. The beam is collimated and expanded, and then reaches the first adjustable mirror 5.

[0035] The intelligent high-speed scanning module incl...

Embodiment 2

[0042] This embodiment provides an intelligent high-speed scanning method for point-scanning long-life fluorescence microscopy imaging, which includes the following steps:

[0043] S1. The excitation light source generates excitation light, which is a Gaussian beam; the excitation light reaches the linear polarizer 3 after being adjusted by the half-wave plate 2, and then passes through the linear polarizer 3 to become linearly polarized light and then passes through Gaussian light expansion. The beam collimating lens group 4 performs collimation and expansion of the beam, then reaches the first adjustable mirror 5, and is reflected by the first adjustable mirror 5 into the X-axis direction acousto-optic modulator;

[0044] S2. The excitation light is firstly modulated by the acousto-optic modulator, then reflected by the second adjustable mirror 8 and scanned the lens 9 and the microscope tube mirror 10 in sequence, and then reaches the third adjustable mirror 11; After being...

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Abstract

The invention discloses an intelligent high-speed scanning method and device for spot scanning long-life fluorescence microscopic imaging. The problems of image deformation and distortion of adjacent pixel points in an excitation light spot area is avoided due to overlong luminous life of a fluorescent material in traditional continuous high-speed scanning by adopting an acousto-optic modulator to generate indirect scanning. Besides, a detector and a motion control card form an open-loop feedback system, a noise signal threshold value in the imaging process is determined, noise signals in imaging are filtered out, and therefore a fluorescence image without deformation and distortion is formed. According to the method, the problems of image deformation and distortion are solved, meanwhile, observation differences are effectively reduced, and data processing difficulty and complexity after imaging are reduced.

Description

technical field [0001] The invention belongs to the field of optical microscopy technology, and specifically relates to an intelligent point scanning method for realizing long-life probe fluorescence imaging without deformation and distortion, and an imaging device based on an intelligent high-speed scanning control system realized by the scanning method. Background technique [0002] Optical fluorescence imaging is one of the most commonly used techniques in microscopic imaging of biological analysis, which has the characteristics of high sensitivity, strong specificity, and friendliness to living biological tissues. Optical fluorescence microscopy can accurately obtain the distribution position of single molecules in cells, detect ion concentrations and observe cell movement processes through specific labeling methods of fluorescent probes, and can also realize the study of protein-molecular interactions. As a cutting-edge technology in optical fluorescence imaging, optica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G02B21/00
CPCG02B21/0036G02B21/0076G02B21/008
Inventor 詹求强唐欢蒲锐文紫照邱雪王保举肖楚
Owner SOUTH CHINA NORMAL UNIVERSITY