Separation method of N-methyl-5-hydroxytryptamine in badam and application of N-methyl-5-hydroxytryptamine in badam
A separation method, nitrogen-methyl technology, applied in the field of chemistry, can solve problems such as which component is unclear, and the mechanism of efficacy cannot be clarified, so as to achieve the effect of excellent sedative and sleep-promoting activity
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Embodiment 1
[0033] The method for extracting and purifying almond nitrogen methyl 5-hydroxytryptamine from almonds, the steps are as follows:
[0034] After crushing almonds, bake them at 105°C for 2 hours, add 70% ethanol at a material-to-liquid ratio of 1:10 (M / V), stir and extract at 50°C for 2 hours, filter to get the supernatant, and repeat this once. The supernatant obtained twice was concentrated under reduced pressure to remove ethanol to obtain the almond extract. Put the almond extract on the macroporous resin AB-8 column, use deionized water, 10%, 20%, and 95% ethanol for gradient elution, collect the 20% ethanol eluted part, and depressurize After concentration, the sample is loaded onto the ODS column, eluted with 10%, 20%, and 30% ethanol, and the 20% ethanol eluted part is collected, and the collected 20% ethanol eluted part is put on the SephadexLH-20 column, and finally a monomer is obtained Compound - nitrogen methyl 5-hydroxytryptamine, the compound is a colorless amor...
Embodiment 2
[0037] The method for extracting and purifying almond nitrogen methyl 5-hydroxytryptamine from almond, its step is as follows:
[0038] After crushing the almond, bake it at 105°C for 2 hours, add 60% ethanol according to the ratio of solid to liquid 1:6 (M / V), stir and extract at 60°C for 1 hour, filter to take the supernatant, and repeat this once. The supernatant obtained twice was concentrated under reduced pressure to remove ethanol to obtain the almond extract. Put the alcohol extract of almond wood on the macroporous resin AB-8 column, and use deionized water, 10%, 20%, and 90% ethanol for gradient elution in sequence, collect the eluted part of 20% ethanol, and depressurize After concentration, the sample is loaded onto the ODS column, eluted with 10%, 20%, and 30% ethanol, and the 20% ethanol eluted part is collected, and the collected 20% ethanol eluted part is put on the SephadexLH-20 column, and finally a monomer is obtained Compound - nitrogen methyl 5-hydroxytry...
Embodiment 3
[0040] The method for extracting and purifying almond nitrogen methyl 5-hydroxytryptamine from almond, its step is as follows:
[0041] After the almond is crushed, bake at 105°C for 2 hours, add 65% ethanol according to the ratio of material to liquid 1:8 (M / V), stir and extract at 55°C for 1.5h, filter and take the supernatant, and repeat this once . The supernatant obtained twice was concentrated under reduced pressure to remove ethanol to obtain the almond extract. Put the alcohol extract of almond wood on the macroporous resin AB-8 column, and use deionized water, 10%, 20%, and 90% ethanol for gradient elution in sequence, collect the eluted part of 20% ethanol, and depressurize After concentration, the sample is loaded onto the ODS column, eluted with 10%, 20%, and 30% ethanol, and the 20% ethanol eluted part is collected, and the collected 20% ethanol eluted part is put on the SephadexLH-20 column, and finally a monomer is obtained Compound - nitrogen methyl 5-hydroxy...
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