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Microorganism capable of dissolving thrombus and targeted phagocytosis of cancer cells

A technology for microorganisms and cancer cells, applied in the field of bioengineering, can solve problems such as the treatment of blood clots without metabolites of microorganisms, and achieve the effect of improving the efficiency of culture

Inactive Publication Date: 2021-10-22
陈元 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the existing technology does not use the metabolites of microorganisms to treat thrombus, and does not use the microorganisms themselves to target and phagocytize cancer cells

Method used

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  • Microorganism capable of dissolving thrombus and targeted phagocytosis of cancer cells
  • Microorganism capable of dissolving thrombus and targeted phagocytosis of cancer cells
  • Microorganism capable of dissolving thrombus and targeted phagocytosis of cancer cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] S1: Microbial activation: connect the microorganisms to a 500ml incubator containing 200ml LB slant medium, place the incubator in an incubator for cultivation, set the temperature of the incubator at 35°C, and cultivate for 24 hours;

[0033] S2: Preparation of seed solution: inoculate the activated microorganisms into a 500ml incubator containing 200ml of LB seed medium with an inoculation loop, and place the incubator in a constant temperature shaker for cultivation. The set temperature of the constant temperature shaker is 35°C, and the speed is 200 rpm, incubation time is 19 hours;

[0034] S3: Fermentation broth preparation: The seed liquid was inoculated into a 250ml incubator containing 75ml of liquid fermentation medium with an inoculation amount of 4% of the fermentation broth volume, and the incubator was placed in a constant temperature shaker for cultivation. For 48 hours, the pH value is 7.0, the rotation speed of the constant temperature shaker is 170 rpm...

Embodiment 2

[0037] S1: Microbial activation: connect the microorganisms to a 500ml incubator containing 200ml LB slant medium, place the incubator in an incubator for cultivation, set the temperature of the incubator at 33°C, and cultivate for 22 hours;

[0038] S2: Preparation of seed liquid: inoculate the activated microorganisms into a 500ml incubator containing 200ml LB seed medium with an inoculation loop, and place the incubator in a constant temperature shaker for cultivation. The set temperature of the constant temperature shaker is 33°C, and the rotation speed is 180 rpm, culture time is 17 hours;

[0039] S3: Fermentation broth preparation: The seed liquid was inoculated into a 250ml incubator containing 75ml of liquid fermentation medium with an inoculation amount of 4% of the volume of the fermentation broth, and the incubator was placed in a constant temperature shaker for cultivation. For 44 hours, the pH value is 7.0, the constant temperature shaker rotates at 150 rpm, and ...

Embodiment 3

[0042] S1: Microbial activation: connect the microorganisms to a 500ml incubator containing 200ml LB slant medium, place the incubator in an incubator for cultivation, set the temperature of the incubator at 37°C, and cultivate for 26 hours;

[0043] S2: Seed solution preparation: Inoculate the activated microorganisms into a 500ml incubator containing 200ml LB seed medium with an inoculation loop, and place the incubator in a constant temperature shaker for cultivation. The set temperature of the constant temperature shaker is 37°C, and the speed is 220 rpm, culture time is 23 hours;

[0044] S3: Fermentation broth preparation: The seed liquid was inoculated into a 250ml incubator containing 75ml of liquid fermentation medium with an inoculation amount of 4% of the volume of the fermentation broth, and the incubator was placed in a constant temperature shaker for cultivation. For 50 hours, the pH value is 7.0, the rotation speed of the constant temperature shaker is 190 rpm, ...

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Abstract

The invention discloses a culture of microorganisms capable of dissolving thrombus and targeted phagocytosis of cancer cells and an application method thereof, the method comprises the following steps: S1, activating microorganisms: inoculating the microorganisms into a 500ml culture vessel containing 200ml of LB slant culture medium, and placing the culture vessel in an incubator for culturing; S2, preparing a seed solution: inoculating activated microorganisms into a 500ml incubator containing 200ml of an LB seed culture medium by using an inoculating loop, and placing the incubator in a constant-temperature shaking table for culturing; and S3, fermentation liquid: inoculating the seed liquid into a 250ml incubator containing 75ml of liquid fermentation culture medium according to the inoculum size of 4% of the volume of the fermentation liquid, and placing the incubator in a constant-temperature shaking table for culturing. According to the invention, the culture efficiency of the microorganism is improved, and a tablet capable of dissolving thrombus is produced by directly utilizing the microorganism, and an injection capable of targeting phagocytosis of cancer cells is produced by directly utilizing the microorganism.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a microorganism capable of dissolving thrombus and targeting cancer cells. Background technique [0002] Thrombosis is a multifactorial change process in which a group of genetic and environmental factors interact and influence each other. The main characteristics of common clinical thrombosis patients are familial heredity, recurrence, severity of symptoms, abnormal thrombosis site, and younger onset time. Cell necrosis refers to passive death that has long been considered to be caused by pathology, such as physical or chemical damage factors, hypoxia and malnutrition, etc., all lead to cell necrosis. The membrane permeability of necrotic cells increases, resulting in cell swelling, organelle deformation or enlargement, no obvious morphological changes in the early nucleus, and finally cell rupture. Cell lysis releases contents and often causes inflammatory ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00A61K35/66A61K9/08A61K9/20A61P35/00A61P7/02
CPCC12N1/00A61K35/66A61K9/08A61K9/0019A61K9/20A61P35/00A61P7/02
Inventor 陈婧文陈元
Owner 陈元
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