Primer group for amplifying children individualized medication gene sites, primer group for detection and application thereof

Abstract

The invention provides a primer group for amplifying children individualized medication gene sites, a primer group for detection and application thereof. The primer group and the single-base extension primer for amplifying the children individualized medication gene sites are obtained by designing 42 SNP loci of 31 medication-related genes of active ingredients of 122 medicines and 32 medicines. The invention also discloses application of the primer group for amplification and the primer group for detection formed by the primer group for amplification and the single-base extension primer or a kit prepared from the primer group for detection in detection of the children individualized medication gene site polymorphism. The primer group for detection covers the types of common medicines for children, contains many effective components of the medicines, contains many genes and SNP sites, has high confidence level of gene sites, is designed and optimized for gene databases of Chinese people, and has the characteristics of high detection sensitivity, high efficiency and high flux.

Description

technical field [0001] The invention belongs to the technical field of gene detection, and in particular relates to a primer set for amplifying gene loci for children's individualized medicine, a primer set for detection and applications thereof. Background technique [0002] The State Food and Drug Administration's "2016 White Paper on Children's Medication Safety Investigation Report" shows that improper medication can have serious consequences. The "National Adverse Drug Reaction Monitoring Annual Report (2016)" shows that in the case reports of adverse drug reactions / events in 2016, children under the age of 14 accounted for a relatively high proportion. [0003] The definition of children's age in the medical field in my country is between 0 and 14 years old. In the narrow sense, children's medicines refer to the medicines that are clearly stated in the drug instructions that they are only for children. In the broad sense, children's medicines refer to medicines that ca...

AI Technical Summary

Problems solved by technology

2) The reliability of the site is low, which brings unsafe hidden dangers to children's safe medication guidance

Because there are differences in the frequency of gene mutations between the East and the West, and different types of mutations have different effects on enzyme activity and drug metabolism, there are many differences in the use of the same drug between Chinese and Westerners. The design and optimization of genetic loci, simply copying foreign literature and data, etc., will cause many ineffective and redundant tests for Chinese people, resulting in waste

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