Cashmere protein marking method and peptide fragment sequence of differential expression protein of cashmere protein

Abstract

The invention discloses a cashmere protein marking method which comprises the following steps: extracting proteins of cashmere and wool of cashmere goats, and measuring the total protein concentration; carrying out enzymolysis on the total protein, recording the enzymolysis condition and the protein content, and analyzing the composition and the content of the peptide fragment by adopting a high performance liquid chromatography-tandem mass spectrometry method; taking peptide fragment data obtained by SWATH mass spectrometry as non-target data, and taking peptide fragment data obtained by an unmarked quantitative method in an IDA mode as target data; and measuring the content of differential expression protein, and identifying the cashmere and the wool by utilizing the content of the differential expression protein. The invention also discloses a peptide fragment sequence of the differential expression protein. The method has the advantages of high sensitivity, wide dynamic range, high scanning speed, high coverage rate, relatively low cost, simplicity and convenience in test operation and no limitation.

Description

technical field [0001] The invention belongs to the technical field of fiber identification, and in particular relates to a cashmere protein labeling method and a peptide sequence of differentially expressed proteins. Background technique [0002] Proteomics is a science that studies the structure, function, composition, expression and activity of proteins. Including protein qualitative and quantitative, protein structure and function, protein post-translational modification, etc. Quantitative Proteomics (Quantitative Proteomics) is a science that accurately identifies and quantifies all proteins expressed in a genome or all proteins in a complex mixed system. It can be used to screen and find differentially expressed proteins between samples caused by any factor. Quantitative proteomics can be divided into two types: labeled quantitative omics and non-labeled quantitative omics. Label-free quantification, that is, Label-free quantification, is usually used to analyze the ...

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Problems solved by technology

For the morphological feature observation and identification method, the manual observation of the scale structure is slow, subject to interference from subjective factors, and results in large errors

The solution infiltration and dyeing identification method will cause great damage to the villi itself, the determination accuracy is limited, and the identification effect is not obvious. It is only suitable for the distinction of fiber macroscopic morphology differences, and the test accuracy is limited.

The reliability of the spectral analysis method depends to a large extent on the establishment of a perfect model and database; the test results largely depend on the integrity of the model database built, the database must be representative, and there must be sufficient data Accumulation, otherwise it is easy to cause adverse effects on the test results

Alkali solubility difference method needs to be carried out under specific test conditions and takes a long time

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