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Application of lncRNA SLC25A21-AS1 as marker of esophageal squamous cell carcinoma

A technology for esophageal squamous cell carcinoma and esophageal squamous cell carcinoma, which is applied in the application field of esophageal squamous cell carcinoma markers, and can solve problems such as complex influencing factors of metabolic syndrome

Pending Publication Date: 2021-11-02
CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Although previous studies have suggested that metabolic syndrome is associated with the prognosis of patients with esophageal squamous cell carcinoma, considering that the influencing factors of metabolic syndrome are very complex, it is proposed to establish an obese animal model through a high-fat diet, and then explore the high-fat diet (High-fat diet). diets, HFD) and the effect of the formed high-fat microenvironment on esophageal squamous cell carcinoma

Method used

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  • Application of lncRNA SLC25A21-AS1 as marker of esophageal squamous cell carcinoma
  • Application of lncRNA SLC25A21-AS1 as marker of esophageal squamous cell carcinoma
  • Application of lncRNA SLC25A21-AS1 as marker of esophageal squamous cell carcinoma

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Experimental program
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Effect test

Embodiment 1

[0065] Example 1. High-fat diet inhibits the growth of esophageal squamous cell carcinoma subcutaneous tumor in nude mice

[0066] 1. High-fat diet inhibits the growth of subcutaneous tumors of esophageal squamous cell carcinoma in nude mice

[0067] The mice used in the subcutaneous tumor formation experiment were BALB / cA-nu nude mice (Beijing Huafukang Company). Select male BALB / cA-nu nude mice, 3-4 weeks old, weighing between 18-22g.

[0068] 1. Construction of high-fat-induced obesity animal model

[0069] 4-week-old male nude mice (BALB / cA-nu) were selected, 10-15 in each group, and randomly divided into two groups, divided into a common diet group (Common diet, CD) and a high-fat diet group (High fat diet, HFD), the earrings are numbered, and a maximum of 5 are placed in each cage.

[0070] After one week of adaptive feeding, the nude mice were weighed for the first time, and the initial body weight was recorded and marked with earrings. The normal diet group feeds t...

Embodiment 2

[0119] Example 2. High-fat-related lncRNA SLC25A21-AS1 and its application in postoperative survival of esophageal squamous cell carcinoma 1. Screening and verification of high-fat-related lncRNA SLC25A21-AS

[0120] To explore the possible mechanism of action of palmitic acid on esophageal squamous cell carcinoma cells by transcriptome sequencing (RNA-seq) analysis, three esophageal squamous cell carcinoma cell lines KYSE30, KYSE450 and KYSE150 were selected (German DSMZ Collection, ACC 351; ACC 387; ACC375), using 100 μM palmitic acid for 24 hours to extract total cellular RNA and send it to sequencing. Through the co-screening of cell line transcriptome sequencing results and database GSE53625, an lncRNA named lncRNA SLC25A21-AS1 (ENSG00000258708) was obtained, which had significant differences in cell line transcriptome sequencing analysis and database.

[0121] Figure 5 The heat map of lncRNAs down-regulated and up-regulated in esophageal squamous cell lines KYSE30, KYS...

Embodiment 3

[0168] Example 3, the biological function of lncRNA SLC25A21-AS1 in the treatment of esophageal squamous cell carcinoma cells

[0169] 1. Knockdown of lncRNA SLC25A21-AS1 inhibits the proliferation and migration of esophageal squamous cell carcinoma cells

[0170] 1. Cell level

[0171] 1) Construction of knockout vector

[0172] In order to explore the effect of lncRNA SLC25A21-AS1 on esophageal squamous cell carcinoma cells, short hairpin RNA (shRNA) was used to knock down the expression of lncRNA SLC25A21-AS1 in KYSE30 and KYSE450 cell lines (German DSMZ Collection, ACC 351; ACC387). The specific method as follows:

[0173] The shRNA (Short hairpin RNA) of lncRNA SLC25A21-AS1 was constructed on the GV654 knockdown vector (element sequence: hU6-MCS-Ubiquitin-mCherry-IRES-Neomycin), and the target region was in the non-overlapping region of lncRNA SLC25A21-AS1 and SLC25A21 genes , synthesized by Beijing Syngene; the knockout vector expressing NC-shRNA, the knockout vector ...

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Abstract

The invention discloses an application of lncRNA (long non-coding ribonucleic acid) SLC25A21-AS1 as a marker of esophageal squamous cell carcinoma. The invention provides application of a substance for detecting the expression quantity of LncRNA SLC25A21-AS1 in at least one of the following functions or preparation of a product with at least one of the following functions: (1) a product for predicting the prognosis state of a patient with esophageal squamous carcinoma; (2) predicting the postoperative recurrence-free survival rate of the esophageal squamous carcinoma patient; (3) predicting the overall survival rate of the esophageal squamous carcinoma patient after operation; (4) predicting the tumor differentiation degree of the esophageal squamous carcinoma patient; experiments prove that the lncRNA SLC25A21-AS1 is used as the lncRNA for promoting tumor development, can influence the progress of esophageal squamous cell carcinoma through multiple ways, and prompts that the lncRNA SLC25A21-AS1 has the multifunctional characteristic, but the expression is reduced under the influence of high fat diet or palmitic acid.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of lncRNA SLC25A21-AS1 as a marker for esophageal squamous cell carcinoma. Background technique [0002] Esophageal cancer is a relatively common malignant tumor in the world. Esophageal squamous cell carcinoma is the most common type of esophageal cancer in my country. Due to its high degree of malignancy, the prognosis of patients is usually poor. Therefore, it is necessary to clarify the pathogenesis and influencing factors of esophageal squamous cell carcinoma. In recent years, with the improvement of living standards, the incidence of obesity and related metabolic syndrome has increased significantly, and it has been reported that it is closely related to the occurrence and development of various tumors, including esophageal squamous cell carcinoma. [0003] Although previous studies have suggested that metabolic syndrome is associated with the progn...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886A61K45/00A61K31/7088A61K31/20A61P35/00A61P35/04
CPCC12Q1/6886A61K45/00A61K31/7088A61K31/20A61P35/00A61P35/04C12Q2600/118C12Q2600/136C12Q2600/158C12Q2600/178
Inventor 赫捷李春香刘宇
Owner CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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