Intestinal flora marker for diagnosing calcium oxalate calculus and application of intestinal flora marker

A technology for intestinal flora and grass diagnosis is applied in the field of intestinal flora markers for diagnosing calcium oxalate stones, which can solve the problems of unclear pathogenesis of calcium oxalate stones and the like

Pending Publication Date: 2021-11-02
GUANGXI MEDICAL UNIVERSITY
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  • Abstract
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Problems solved by technology

[0003] As a disease caused by multiple etiologies, the pathogenesis of calcium oxalate stones is still unclear

Method used

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  • Intestinal flora marker for diagnosing calcium oxalate calculus and application of intestinal flora marker
  • Intestinal flora marker for diagnosing calcium oxalate calculus and application of intestinal flora marker
  • Intestinal flora marker for diagnosing calcium oxalate calculus and application of intestinal flora marker

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Embodiment 1

[0027] Example 1: Screening of intestinal flora associated with calcium oxalate stones

[0028] 1. Research object and sample collection

[0029] Twenty-four patients with calcium oxalate stones and 24 co-living healthy controls (HC) were collected from Tongji Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province.

[0030] Inclusion criteria: patients with calcium oxalate stones were screened through stone component analysis, and healthy controls were screened through urinary ultrasound and medical history inquiry.

[0031] Exclusion criteria: (1) Age <18 years; (2) Antibiotic use within 3 months before sampling.

[0032] Clinical characteristics of the samples: 14 of the patients were male, and 5 of the healthy controls were male.

[0033] Stool samples were collected from study subjects.

[0034] 2. DNA extraction and sequencing

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Abstract

The invention discloses an intestinal flora marker for diagnosing calcium oxalate calculus and application of the intestinal flora marker. The invention relates to an application of a reagent for detecting the abundance of Burkholderia pseudomallei and / or Bacteroides sp.CAG:443 in the preparation of a reagent for diagnosing calcium oxalate calculus. According to the present invention, it is found for the first time that Burkholderia pseudomacrolei and Bacteroides sp.CAG:443 are related to calcium oxalate calculus, the abundance of Burkholderia pseudomacrolei and Bacteroides sp.CAG:443 has significant difference in calcium oxalate calculus patients and healthy people, ROC curve analysis results show that the Burkholderia pseudomacrolei and Bacteroides sp.CAG:443 as detection variables have high specificity and high sensitivity, such that Burkholderia pseudomacrolei and / or Bacteroides sp.CAG:443 can be used as detection markers for diagnosis of calcium oxalate calculus patients. Burkholderia pseudomacrolei and / or Bacteroides sp.CAG:443 are / is used as a detection marker, so that the marker is completely noninvasive and is high in accuracy.

Description

Technical field: [0001] The invention belongs to the field of biomedicine, and in particular relates to an intestinal flora marker for diagnosing calcium oxalate stones and an application thereof. Background technique: [0002] Urinary calculus is a common disease in urology, ranking first among inpatients ("Guidelines for the Diagnosis and Treatment of Urology and Andrology Diseases in China" (2019 Edition)). There are many factors that affect stone formation, including age, gender, race, genetics, environment, eating habits, and occupation. Paying attention to the factors that form stones can reduce the formation and recurrence of stones. Among them, metabolic abnormalities related to stone components have attracted more and more attention. Epidemiological survey results show that more than 80% of stones are calcium oxalate stones. [0003] As a disease caused by multiple etiologies, the pathogenesis of calcium oxalate stones is still unclear. It is now found that micro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/689C12Q1/04C12N15/11C12N1/20C12R1/01
CPCC12Q1/689C12N1/20
Inventor 徐华张宇聪陶芝伟刘浩然
Owner GUANGXI MEDICAL UNIVERSITY
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