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Infection identification of pig trophoblast line used for pseudorabies virus replication

A technique for porcine pseudorabies virus and trophoblast, applied in the field of infection identification of swine trophoblast

Pending Publication Date: 2021-11-02
SOUTH CHINA AGRI UNIV
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Problems solved by technology

In the prior art, the research on PRV on porcine trophoblast cells has not yet been carried out, and the research needs to be accelerated in the later stage

Method used

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  • Infection identification of pig trophoblast line used for pseudorabies virus replication
  • Infection identification of pig trophoblast line used for pseudorabies virus replication
  • Infection identification of pig trophoblast line used for pseudorabies virus replication

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Embodiment Construction

[0012] The present invention will be described below with reference to specific examples. Those skilled in the art can understand that these examples are only used to illustrate the present invention and do not limit the scope of the present invention in any way.

[0013] Infection identification of a porcine trophoblast line that can be used for porcine pseudorabies virus replication, using immortalized porcine embryonic trophoblast cells as a novel organelle to study the link between porcine pseudorabies virus and reproductive disorders, immortalized trophoblast The cell line can be efficiently / stablely passaged, and the culture system is mature.

[0014] In order to observe the cytopathy caused by PRV-infected trophoblast cells, the trophoblast cells obtained in this example were subjected to a comparative experiment of PRV infection, and were divided into an infected group (MOI=1 group) and a non-infected virus control group (Mock group). The result is as figure 1 As sho...

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Abstract

The invention discloses infection identification of a pig trophoblast line used for pseudorabies virus replication. Immortalized pig embryo trophoblast cells are adopted as novel organelles to research the relation between the pig pseudorabies virus and reproductive disorder diseases, the immortalized trophoblast cell line can be efficiently / stably passaged, and a culture system is mature. The cell line can support replication of PRV, it is determined that the PRV can well infect immortalized pig embryo trophoblast cells and cause cytopathic effects, the difference between the PRV and TCID50 of PRV infected passage cells PK15 is less than one titer, and the cell line can be used for researches on the aspects of passage and receptors of the PRV, mechanisms of reproductive disorder diseases caused by the PRV and the like. The establishment of the passage trophoblast cell line in the invention is of great significance to the vertical propagation research of pig-related viruses and the related research on pig breeding.

Description

technical field [0001] The invention belongs to the technical field of biological products, and in particular relates to an infection identification of porcine trophoblast system which can be used for porcine pseudorabies virus replication. Background technique [0002] Porcine pseudorabies (Pseudorabies, PR) is an acute and highly contagious infectious disease of pigs that occurs worldwide. The disease is highly contagious, and it is prone to mixed infection or secondary infection of bacteria and other viruses. It mainly causes acute death of newborn piglets and neurological symptoms in piglets over four weeks old. Pregnant sows suffer from reproductive disorders, abortion, stillbirth and respiratory symptoms. as a feature. The pathogen of PR is pseudorabies virus (Pseudorabiesvirus, PRV), which belongs to the herpesvirus type I of the varicellavirus genus of the αherpesvirinae subfamily of the family Herpesviridae, and the full length of the genome is 150kbp. In the prio...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N33/569
CPCG01N21/6486G01N21/6428G01N33/56983G01N2021/6439
Inventor 宋长绪刘献辉洪林君张歆明徐舸熊谋康张乐宜刘艳玲徐铮
Owner SOUTH CHINA AGRI UNIV
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