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Ultrafast dynamic observation system and method based on cryoelectron microscope

An electron microscope and observation system technology, applied in measurement devices, material analysis using wave/particle radiation, instruments, etc., can solve the problem that the dynamic change process of biological samples cannot be observed in situ.

Pending Publication Date: 2021-11-02
WUHAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] By providing an ultrafast dynamic observation system and method based on a cryo-electron microscope, the present invention solves the problem that the existing technology cannot observe the dynamic change process of biological samples in situ under the condition of external excitation

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  • Ultrafast dynamic observation system and method based on cryoelectron microscope

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Embodiment 1

[0040] Embodiment 1 provides a kind of ultrafast dynamic observation method based on cryo-electron microscopy, comprising the following steps:

[0041] Step 1. A laser is used to emit laser pulses and excite the sample in the cryo-electron microscope to generate dynamic changes.

[0042] Wherein, the sample is a biological sample to be observed after rapid freezing treatment. The laser can be a femtosecond laser or a picosecond laser, and preferably a femtosecond laser. Ultrashort pulses of the femtosecond laser are more conducive to exciting biological samples.

[0043] For example, a femtosecond laser is used to emit a laser pulse with a wavelength of 1040nm, and the 1040nm laser pulse is frequency-doubled to obtain a 520nm or 260nm femtosecond laser pulse to excite the sample to generate dynamic changes.

[0044] Step 2: Imaging the sample to obtain at least one detection information among in-situ change information of the sample and ultrafast kinetic change information of...

Embodiment 2

[0052] Embodiment 2 is a system corresponding to the method provided in Embodiment 1, and the system is used to implement the steps of the method in Embodiment 1.

[0053] Embodiment 2 provides an ultrafast dynamic observation system based on a cryo-electron microscope, including: a cryo-electron microscope, a laser, an excitation optical path and an imaging optical path. The cryo-electron microscope is used to place a fixed sample, and the sample is a biological sample to be observed after undergoing rapid freezing treatment; the laser is used to emit a laser pulse with a first wavelength; Laser pulses of two wavelengths are introduced into the cryo-electron microscope to excite the sample located in the cryo-electron microscope to produce dynamic changes; In situ imaging of the sample before and after laser pulse excitation to obtain in situ change information of the sample; the imaging optical path is used to introduce a laser pulse with a third wavelength into the cryo-ele...

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Abstract

The invention belongs to the technical field of cryoelectron microscopes, and discloses an ultrafast dynamic observation system and method based on a cryoelectron microscope. The method comprises the following steps: firstly, emitting a laser pulse by using a laser, and exciting a sample in a cryoelectron microscope to generate dynamic change, wherein the sample is a biological sample to be observed after rapid freezing treatment; and then imaging the sample to obtain at least one detection information of sample in-situ change information and sample ultrafast dynamic change information. The problem that in the prior art, the dynamic change process of the biological sample under the external excitation condition cannot be observed in situ is solved, and the method has important significance for researching the dynamic change process of the biological sample.

Description

technical field [0001] The invention belongs to the technical field of cryo-electron microscopy, and more specifically relates to an ultrafast dynamic observation system and method based on a cryo-electron microscope. Background technique [0002] Biomedicine has achieved rapid development in recent years, especially the research on macromolecules such as biological proteins and DNA, which is of great significance to life science research and drug research and development. Even biological macromolecules are nanoscale in size, and how to observe and understand their structures is a difficult problem. [0003] Scientists have been trying to see the structure of proteins, but for a long time, only get a blurry picture. Originally, X-rays were used to analyze biological structures. In order to use X-ray imaging, protein molecules need to clump together to form stable crystals, but many proteins that are crucial to the human body are too loose to clump together to form crystals....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N23/2251G01N23/2202
CPCG01N23/2251G01N23/2202
Inventor 刘胜潘俊衡汪启军
Owner WUHAN UNIV