Ratio fluorescence immunochromatography detection test strip and detection method thereof

Abstract

The invention provides a ratio fluorescence immunochromatography detection test strip. The test strip comprises a sample pad, a marking pad, a coating film and a water absorption pad which are connected in sequence; a calibration line C, a test line T1 and a test line T2 are sequentially arranged on the coating film, and the line C is coated with an independent quality control antibody; the marking pad contains a fluorescently-labeled recombinant antigen and a fluorescently-labeled independent quality control antigen; the T1 line is coated with a recombinant antigen; and the T2 line is coated with a recombinant antigen ligand. According to the invention, the ratio (T1 / T2) of the fluorescence detection signals on the T1 line and the T2 line is determined, the ratio (T1 / T2) is positively correlated with the antibody concentration in the sample, and a correlation calibration curve equation is established so as to establish a qualitative or quantitative detection fluorescence immunochromatography detection method; according to the test strip and the method provided by the invention, the interference of a fluorescence detection background signal is overcome, and the sensitivity, the accuracy and the credibility of fluorescence immunochromatography are improved.

Description

technical field [0001] The invention relates to the field of medical testing, in particular to a ratiometric fluorescent immunochromatographic detection test strip and a detection method thereof. Background technique [0002] Fluorescence immunochromatography rapid detection technology is based on the principle of immunochromatography and is suitable for on-site detection. Typical fluorescence immunochromatography test strips include sample pads, fluorescent marker pads, coating membranes and absorbent pads. Fluorescent immunochromatography uses nitrocellulose membrane as a solid-phase carrier, through permeation and capillary siphoning, the antibody or antigen in the sample to be tested is combined with the antigen or antibody coated on the membrane, and the reaction is presented by the marker on the fluorescent marker pad result. This technique has the advantages of simple operation, rapid detection and strong portability. [0003] For the detection of infectious disease...

AI Technical Summary

Problems solved by technology

[0004] However, due to the complex chromatographic process of the test strip, the hydrophobic adsorption of the nitrocellulose membrane, the electrostatic attraction of the coating material and other factors will cause a certain background of the fluorescence detection signal.

If the fluorescence detection background signal is too high, it will cause a large deviation in the detection results, so that the content of the substance to be tested cannot be accurately and truly reflected, and the sensitivity of the detection will also be reduced.

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