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Method for preparing porous calcium alginate microspheres by using micro-fluidic aqueous two-phase emulsion as template

A calcium alginate, two-phase technology, which is applied in the chemical method of reacting liquid with liquid, microsphere preparation, microcapsule preparation, etc. The problem of low biocompatibility is to achieve the effect of flexible and convenient production, easy flow rate and air pump air pressure, and flow rate and air pump air pressure adjustment.

Active Publication Date: 2021-11-12
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to solve the above-mentioned technical problems, the object of the present invention is to provide a method for preparing porous calcium alginate microspheres using microfluidic two-phase emulsion as a template to solve the problem of the biological phase of porous calcium alginate microspheres prepared by existing preparation methods. The problem of low capacity and poor control of microsphere diameter and pore size

Method used

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  • Method for preparing porous calcium alginate microspheres by using micro-fluidic aqueous two-phase emulsion as template
  • Method for preparing porous calcium alginate microspheres by using micro-fluidic aqueous two-phase emulsion as template
  • Method for preparing porous calcium alginate microspheres by using micro-fluidic aqueous two-phase emulsion as template

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Embodiment 1

[0054] A method for preparing porous calcium alginate microspheres using a microfluidic two-phase emulsion as a template, comprising the following steps:

[0055] (1) The PEG solution and the Dex solution with the mass fraction of equal volume being 16% are fully mixed on the rotary incubator, after standing for 6 hours to separate the phases, the upper phase is extracted to obtain the PEG aqueous solution, and the lower phase is extracted to be the Dex aqueous solution, wherein , PEG molecular weight is 8kDa, Dex molecular weight is 500kDa;

[0056] (2) Add CaCl to the PEG aqueous solution obtained in step (1) 2 , after mixing, the CaCl 2 PEG-CaCl with a mass fraction of 10% 2 solution;

[0057] (3) adding Alg to the Dex aqueous solution obtained in step (1), and mixing to obtain a Dex-Alg solution with an Alg mass fraction of 1%;

[0058] (4) Measure PEG aqueous solution and Dex-Alg solution with a volume ratio of 1:3, and use a portable high-speed disperser to emulsify ...

Embodiment 2

[0062] A method for preparing porous calcium alginate microspheres using a microfluidic two-phase emulsion as a template, comprising the following steps:

[0063] (1) The PEG solution and the Dex solution with the mass fraction of equal volume being 16% are fully mixed on the rotary incubator, after standing for 6 hours to separate the phases, the upper phase is extracted to obtain the PEG aqueous solution, and the lower phase is extracted to be the Dex aqueous solution, wherein , PEG molecular weight is 8kDa, Dex molecular weight is 500kDa;

[0064] (2) Add CaCl to the PEG aqueous solution obtained in step (1) 2 , after mixing, the CaCl 2 PEG-CaCl with a mass fraction of 10% 2 solution;

[0065] (3) adding Alg to the Dex aqueous solution obtained in step (1), and mixing to obtain a Dex-Alg solution with an Alg mass fraction of 1%;

[0066] (4) Measure PEG aqueous solution and Dex-Alg solution with a volume ratio of 1:3, and use a portable high-speed disperser to emulsify ...

Embodiment 3

[0070] A method for preparing porous calcium alginate microspheres using a microfluidic two-phase emulsion as a template, comprising the following steps:

[0071] (1) The PEG solution and the Dex solution with the mass fraction of equal volume being 16% are fully mixed on the rotary incubator, after standing for 6 hours to separate the phases, the upper phase is extracted to obtain the PEG aqueous solution, and the lower phase is extracted to be the Dex aqueous solution, wherein , PEG molecular weight is 8kDa, Dex molecular weight is 500kDa;

[0072] (2) Add CaCl to the PEG aqueous solution obtained in step (1) 2 , after mixing, the CaCl 2 PEG-CaCl with a mass fraction of 10% 2 solution;

[0073] (3) adding Alg to the Dex aqueous solution obtained in step (1), and mixing to obtain a Dex-Alg solution with an Alg mass fraction of 1%;

[0074] (4) Measure PEG aqueous solution and Dex-Alg solution with a volume ratio of 1:3, and use a portable high-speed disperser to emulsify ...

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Abstract

The invention discloses a method for preparing porous calcium alginate microspheres by using a micro-fluidic aqueous two-phase emulsion as a template, which comprises the following steps that: with a PEG aqueous solution as an external water phase and a PEG / Dex-Alg emulsion as an internal water phase, PEG / Dex-Alg emulsion liquid drops are generated at the conical tip of a coaxial capillary device, wherein the external water phase is continuously injected, and the internal water phase is periodically and intermittently injected; and the generated PEG / Dex-Alg emulsion liquid drops are introduced into a PEG-CaCl2 solution for ionic crosslinking to generate porous calcium alginate microspheres. The diameter of the porous microspheres is controlled by changing the air pressure of the internal water phase input into a micro-fluidic device, and the aperture of the porous microspheres is controlled by changing the volume ratio of the two water-phase emulsions forming an emulsion solution and the oscillation frequency of emulsion formation. The prepared microspheres are high in biocompatibility, the preparation process is simple, and the size of the microspheres and the aperture of the microspheres can be flexibly controlled.

Description

technical field [0001] The invention relates to the technical field of preparation of porous calcium alginate microspheres, in particular to a method for preparing porous calcium alginate microspheres using a microfluidic two-phase emulsion as a template. Background technique [0002] Porous microspheres are a type of microspheres with certain interconnected or independently closed pores on the surface or inside of the sphere. The interconnected inner and outer pores of the porous microspheres lead to a very low mass density and a huge specific surface area, as well as the advantages of adjustable pore size and good fluidity, so the porous microspheres are used in tissue regeneration scaffolds, Abundant application results have been achieved in the fields of targeted drug delivery, drug screening, cell separation, immobilized enzymes, and high-speed chromatography. [0003] Emulsion template method is a method for preparing porous microspheres that has developed vigorously ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J19/00B01J13/14B01J14/00B01L3/00C08B37/04
CPCB01J19/0093B01J13/14B01J14/00B01L3/502784C08B37/0084
Inventor 王垚磊杜婷洪美莹郝昕刘刚杨峰王冠雄何华桃
Owner SOUTHWEST JIAOTONG UNIV