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A method for preparing porous calcium alginate microspheres using microfluidic aqueous two-phase emulsion as a template

A technology of calcium alginate and two-phase water, which is applied in the chemical method of liquid-liquid reaction, microsphere preparation, microcapsule preparation, etc., which can solve the problem of poor control of microsphere diameter and pore size, and porous calcium alginate microspheres. Biocompatibility is not high, to achieve the effect of flexible and convenient production, easy flow rate and air pump pressure, and simple device structure

Active Publication Date: 2022-07-29
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to solve the above-mentioned technical problems, the object of the present invention is to provide a method for preparing porous calcium alginate microspheres using microfluidic two-phase emulsion as a template to solve the problem of the biological phase of porous calcium alginate microspheres prepared by existing preparation methods. The problem of low capacity and poor control of microsphere diameter and pore size

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  • A method for preparing porous calcium alginate microspheres using microfluidic aqueous two-phase emulsion as a template
  • A method for preparing porous calcium alginate microspheres using microfluidic aqueous two-phase emulsion as a template
  • A method for preparing porous calcium alginate microspheres using microfluidic aqueous two-phase emulsion as a template

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Embodiment 1

[0054] A method for preparing porous calcium alginate microspheres with a microfluidic two-phase emulsion as a template, comprising the following steps:

[0055] (1) fully mix the PEG solution and Dex solution with equal volume of 16% mass fraction on the rotary incubator, and after standing for 6 hours to separate the phases, extract the upper phase to obtain the PEG aqueous solution, and extract the lower phase to be the Dex aqueous solution, wherein , the molecular weight of PEG is 8kDa, and the molecular weight of Dex is 500kDa;

[0056] (2) Add CaCl to the PEG aqueous solution obtained in step (1) 2 , after mixing, CaCl was prepared 2 10% PEG-CaCl 2 solution;

[0057] (3) adding Alg to the Dex aqueous solution obtained in step (1), and after mixing, to obtain a Dex-Alg solution with an Alg mass fraction of 1%;

[0058] (4) Measure the PEG aqueous solution and Dex-Alg solution with a volume ratio of 1:3, use a portable high-speed disperser to emulsify for 30s with an o...

Embodiment 2

[0062] A method for preparing porous calcium alginate microspheres with a microfluidic two-phase emulsion as a template, comprising the following steps:

[0063] (1) fully mix the PEG solution and Dex solution with equal volume of 16% mass fraction on the rotary incubator, and after standing for 6 hours to separate the phases, extract the upper phase to obtain the PEG aqueous solution, and extract the lower phase to be the Dex aqueous solution, wherein , the molecular weight of PEG is 8kDa, and the molecular weight of Dex is 500kDa;

[0064] (2) Add CaCl to the PEG aqueous solution obtained in step (1) 2 , after mixing, CaCl was prepared 2 10% PEG-CaCl 2 solution;

[0065] (3) adding Alg to the Dex aqueous solution obtained in step (1), and after mixing, to obtain a Dex-Alg solution with an Alg mass fraction of 1%;

[0066] (4) Measure the PEG aqueous solution and Dex-Alg solution with a volume ratio of 1:3, use a portable high-speed disperser to emulsify for 30s with an o...

Embodiment 3

[0070] A method for preparing porous calcium alginate microspheres with a microfluidic two-phase emulsion as a template, comprising the following steps:

[0071] (1) fully mix the PEG solution and Dex solution with equal volume of 16% mass fraction on the rotary incubator, and after standing for 6 hours to separate the phases, extract the upper phase to obtain the PEG aqueous solution, and extract the lower phase to be the Dex aqueous solution, wherein , the molecular weight of PEG is 8kDa, and the molecular weight of Dex is 500kDa;

[0072] (2) Add CaCl to the PEG aqueous solution obtained in step (1) 2 , after mixing, CaCl was prepared 2 10% PEG-CaCl 2 solution;

[0073] (3) adding Alg to the Dex aqueous solution obtained in step (1), and after mixing, to obtain a Dex-Alg solution with an Alg mass fraction of 1%;

[0074] (4) Measure the PEG aqueous solution and Dex-Alg solution with a volume ratio of 1:3, use a portable high-speed disperser to emulsify for 30s with an o...

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Abstract

The invention discloses a method for preparing porous calcium alginate microspheres by using a microfluidic two-phase emulsion as a template. The coaxial capillary device generates PEG / Dex-Alg emulsion droplets at the tip of the cone, wherein the outer water phase is continuously injected, and the inner water phase is periodically intermittently injected. The generated PEG / Dex-Alg emulsion droplets are Pass PEG-CaCl 2 Ionic crosslinking is carried out in the solution, that is, porous calcium alginate microspheres are generated. By changing the pressure of the inner water phase input into the microfluidic device, the size of the diameter of the porous microspheres is controlled. The volume ratio and the oscillating frequency of emulsification control the size of the pore size of the porous microspheres. The microspheres prepared by the invention have high biocompatibility and simple preparation process, and the size of the microspheres and the pore size of the microspheres can be flexibly controlled.

Description

technical field [0001] The invention relates to the technical field of preparation of porous calcium alginate microspheres, in particular to a method for preparing porous calcium alginate microspheres by using a microfluidic two-phase emulsion as a template. Background technique [0002] Porous microspheres are a type of microspheres with certain interconnected or independent closed pores on the surface or inside of the sphere. The interconnected inner and outer pores of porous microspheres lead to its very low mass density and huge specific surface area, as well as the advantages of adjustable pore size and good fluidity, so porous microspheres are used in tissue regeneration scaffolds, Abundant application results have been achieved in the fields of targeted drug delivery, drug screening, cell separation, immobilized enzymes, and high-speed chromatography. [0003] Emulsion template method is a method for preparing porous microspheres which has been vigorously developed i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J19/00B01J13/14B01J14/00B01L3/00C08B37/04
CPCB01J19/0093B01J13/14B01J14/00B01L3/502784C08B37/0084
Inventor 王垚磊杜婷洪美莹郝昕刘刚杨峰王冠雄何华桃
Owner SOUTHWEST JIAOTONG UNIV