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Sample component extraction device and method

An extraction device and extraction method technology, applied in biochemical cleaning devices, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve the problem of low recovery rate of magnetic beads

Pending Publication Date: 2021-11-12
深圳市刚竹智造科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Based on this, it is necessary to provide a sample component extraction device and method for the problem of low recovery rate of magnetic beads when extracting nucleic acid by magnetic bead method

Method used

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  • Sample component extraction device and method
  • Sample component extraction device and method
  • Sample component extraction device and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] An embodiment of the present invention provides a sample component extraction device for extracting a sample solution in the kit 100. The magnetic particles of the sample ingredients required for adsorption in the sample solution can produce a magnetic field to adsorb the magnetic particles to make the sample ingredients from the sample solution. In this embodiment, the magnetic particles are magnetic beads. Sample solutions need to be extracted sample ingredients as nucleic acids.

[0027] See figure 1 As shown, the kit 100 has an open end 110 open, and the other end closed closure end 120 and the hollow chamber 130, the chamber 130 communicates with the opening of the opening end 110. The chamber 130 is used to dress a sample solution for ingredient extraction.

[0028] See figure 2 As shown, the kit 100 has a set length and the cross-sectional dimension in the longitudinal direction is different. In order to facilitate understanding and description, the kit 100 is sequen...

Embodiment 2

[0062] Unlike Example 1, in the present embodiment, the auxiliary permanent magnet 700 selected.

[0063] Component extracting means includes a sample rotation of the magnet assembly (not shown) and the displacement of the magnet assembly (not shown).

[0064] Rotation of the magnet assembly connected to drive the auxiliary magnet 700, the magnet 700 for relative rotation of the auxiliary reagent cartridge 100, so that the auxiliary magnet 700 is close to the bottom end 100 of the kit The kit of the magnetic assembly located within the kit 100 100 500 near the bottom One end side of the magnetic poles having the same or different.

[0065] Rotation of the magnet assembly having a power element, the power element may be employed to provide devices powered motor, air cylinder and a hydraulic cylinder or the like as a driving force. The power transmitting member through a power transmission assembly corresponding to the output of the auxiliary magnet 700 for rotation. Drive assembly ...

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Abstract

The invention relates to a sample component extraction device and method. The sample component extraction device is used for performing component extraction on a sample solution in a kit. The kit is provided with an open end and a closed end which are opposite to each other. The sample component extraction device comprises an adsorption unit, wherein the adsorption unit comprises a magnetic component and an auxiliary magnet; the magnetic component can extend into different positions of the kit from the open end of the kit so as to adsorb magnetic particles in a sample solution; and the auxiliary magnet is located below the closed end of the kit, and variable magnetic induction intensity exists between the auxiliary magnet and the magnetic component. The auxiliary magnet is arranged at the bottom end of the kit, and the auxiliary magnet and the magnetic component extending into the kit can generate the variable magnetic induction intensity between the two relatively close ends, so that the magnetic particles in the sample solution at different positions of the kit are magnetically attracted, the magnetic particles in the sample solution can be extracted to the maximum extent, and extraction time is saved.

Description

Technical field [0001] The present invention relates to the field of biomedical instruments, and more particularly to a sample component extraction device and method. Background technique [0002] With the development of biotechnology, sample biologically active substance extraction and method of fully automatic biologically active substances are rapidly developed. Among them, the use of magnetic bead method extraction nucleic acid is a new extraction technology in recent years. The core of the magnetic bead is micronized iron oxide, which can be adsorbed by the magnet. The outer surface of the magnetic bead is wrapped in the silica film layer, and the silicon film can specifically adsorb nucleic acid in a high concentration salt environment; the magnetic beads of the silica film in a low salt environment are detached from the nucleic acid, and the extraction and metastasis of the nucleic acid can be achieved. operate. [0003] The kit with a magnetic bead method extracts the nuc...

Claims

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Application Information

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IPC IPC(8): C12M1/42C12M1/36C12M1/00C12N15/10
CPCC12N15/1013C12N15/101
Inventor 白晨
Owner 深圳市刚竹智造科技有限公司
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