Cell tissue culture chip for multi-force-field coupling
A cell tissue and chip technology, which is applied in tissue cell/virus culture devices, stress-stimulated microbial growth methods, bioreactor/fermenter combinations, etc., can solve the problems of rare cell tissue culture chips and achieve robustness Good results
Pending Publication Date: 2021-11-12
FUDAN UNIV
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Problems solved by technology
[0004] At present, there are few research reports on cell and tissue culture chips with multi-force field coupling for mechanical mechanics, fluid mechanics and hydrostatic pressure at home and abroad.
Method used
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Embodiment 1
[0035] The cell tissue culture chip of the present invention is used for mechanical mechanics, hydrodynamics and hydrostatic pressure multi-force field coupling. The chip is fixed on the sliding platform of the parallel pneumatic finger cylinder through screws and three-hole chip clamps. The gas control system is connected, and the control system controls the switch of the solenoid valve and the voltage application time to drive the stretching and pressurization of the chip.
[0036] Pour the liquid PDMS prefabricated solution (A glue: B glue = 10:1) into the pre-prepared chip mold, place it on the horizontal platform inside the blast oven at 65°C, heat and cure for 90min, and take it out; take it out from the mold chip, use a 2mm chip puncher on the figure 1 The shown upper layer PDMS chip (2) enters (10) and the position of the liquid outlet through hole (11) is punched, using a 3mm chip puncher in the figure 1 The shown upper layer PDMS chip (2) and the screw through hole ...
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The invention belongs to the technical field of biology, relates to a cell tissue culture chip for multi-force-field coupling, in particular to a cell tissue culture chip for coupling of multiple force fields of mechanical mechanics, fluid mechanics and hydrostatic pressure. The chip is composed of an upper polydimethylsiloxane (PDMS) chip layer and a lower PDMS chip layer which are bonded with each other, a liquid inlet, a liquid outlet, a three-hole chip clamp and a parallel pneumatic finger cylinder, and the upper PDMS chip layer and the lower PDMS chip layer are cuboids with the same size and are attached to each other in a plasma bonding mode. According to the chip, the air cylinder is connected with an air pressure control system, the chip is connected with a hydraulic control system, and the control system is used for operating opening and closing of an electromagnetic valve and voltage applying time to drive stretching movement of the chip and internal pressurization of the chip. The chip is simple and convenient to operate, can realize dynamic culture of cell tissues and simultaneously apply multi-force-field stimulation, and is applicable to the field of cell tissue mechanics.
Description
technical field [0001] The invention belongs to the field of biotechnology, and relates to a multi-force field coupling cell tissue culture chip, in particular to a multi-force field coupling cell tissue culture chip for mechanical mechanics, fluid mechanics and hydrostatic pressure. Background technique [0002] Traditional cell and tissue culture techniques include static two-dimensional culture of cells and tissues using culture dishes, culture flasks and orifice plates; The behavior and function of the tissue play a decisive role. Research practice shows that the traditional static culture method is difficult to provide a real microenvironment for the cell tissue in vitro, so that the research results of cell tissue biology in vitro are far from those in vivo. . With the development of microfluidic chip technology, the combination of microfluidic chip technology and cell tissue culture technology provides the possibility of simulating the microenvironment of cell tissue...
Claims
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Login to View More IPC IPC(8): C12M3/00C12M1/36C12M1/00
CPCC12M35/04C12M23/16C12M41/40C12M41/48
Inventor 张炜佳颜世强许柯华
Owner FUDAN UNIV