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Application of BUB1 protein in preparation of high-grade meningioma prognosis evaluation reagent or kit

A technology for prognostic evaluation, 1. BUB1, applied in the field of medical biological detection, can solve the problems of no BUB1, etc., and achieve the effect of high repeatability, sensitive detection, and easy operation

Pending Publication Date: 2021-11-12
SHANGHAI CHANGZHENG HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There is no literature report on the application of BUB1 in high-grade meningioma

Method used

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  • Application of BUB1 protein in preparation of high-grade meningioma prognosis evaluation reagent or kit
  • Application of BUB1 protein in preparation of high-grade meningioma prognosis evaluation reagent or kit
  • Application of BUB1 protein in preparation of high-grade meningioma prognosis evaluation reagent or kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Randomly selected postoperative tumor tissues from 72 patients with high-grade meningioma, postoperative tumor tissues from 15 patients with WHO grade I meningioma, and 9 partial dura mater paraffin sections to be resected due to trauma (the tumor tissue sections were all from Shanghai Changzheng Hospital, by 2 The diagnosis was made by a pathologist; the dura mater was obtained from the dura mater of traumatic brain injury patients who needed to be removed due to their illness, and the specimens were obtained through the approval of the hospital ethics committee).

[0041] In this example, the immunohistochemical method was used to detect and score the expression of BUB1 protein in high-grade and low-grade meningioma tissues and normal dura mater. The specific steps are as follows:

[0042] (1) Paraffin sections of high-grade meningioma tissue were prepared, and the oven was set at 60°C overnight;

[0043] (2) Section dewaxing and hydration (xylene①10min→xylene②10min→x...

Embodiment 2

[0059] The surgically resected tumor specimens of patients with high-grade meningioma were randomly selected, and tissue sections of tumors of patients with high-grade meningioma were prepared, and the relative expression of BUB1 protein in high-grade meningioma tissues was detected by using the experimental steps of the immunohistochemical method in Example 1 Calculate the immunohistochemical score.

[0060] A total of 72 cases of high-grade meningioma tissues were detected, and it was found that the expression score of BUB1 in 47 cases of tumor tissue was less than or equal to 3 points, and the expression score of 25 cases of tumor tissue was greater than 3 points. According to the level of BUB1 protein expression (when the final immunohistochemical score is greater than 3, it is high expression, and if it is less than or equal to 3, it is low expression), and the patients with high-grade meningioma are divided into groups, and the progression-free survival curve is drawn ( ...

Embodiment 3

[0062] Sample: A tissue section of a tumor from a patient with high-grade meningioma. The above-mentioned immunohistochemical method was used to detect the relative expression of BUB1 protein in the high-grade meningioma tissue. Micrographs of high-grade meningiomas such as image 3 shown. The calculated immunohistochemical score of this tissue was 6.

[0063] According to postoperative follow-up, the patient's tumor recurred 6 months after the operation.

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Abstract

The invention relates to the technical field of medical biological detection, in particular to application of BUB1 protein in the preparation of a high-grade meningioma prognosis evaluation reagent or kit. The expression level of the BUB1 protein in a high-grade meningioma tissue is determined by utilizing an immunohistochemical technology and system scoring, the correlation between the expression level of the BUB1 protein and the prognosis of a high-grade meningioma patient after an operation is determined by combining post-operation follow-up visit information, and the BUB1 protein can be used for preparing a protein molecular marker for judging the prognosis of the high-grade meningioma patient. And the reagent or kit also has important guiding significance for molecular typing of high-grade meningioma, postoperative monitoring of patients and sequential treatment.

Description

technical field [0001] The present invention relates to the technical field of medical biological detection, in particular to the new application of BUB1 protein, in particular to the application of BUB1 protein in the preparation of high-grade meningioma prognosis assessment reagents or kits. Background technique [0002] Meningioma is one of the most common primary central nervous system tumors. Although most meningiomas appear benign and can be cured by surgical resection, tumor recurrence or progression occurs in 20% of patients. Aggressive histologic features (ie, WHO grade II, III tumors characterized by increased proliferative and mitotic activity, increased cellular atypia, invasion of brain tissue) are the main risk of meningioma recurrence. High-grade meningioma still faces problems such as high recurrence rate and high disability rate, which seriously affect the clinical prognosis and quality of life of patients. [0003] The prognosis of patients with high-grad...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/573
CPCG01N33/57407G01N33/573
Inventor 张旭陈菊祥王洪祥易璐洪帆马佩佩
Owner SHANGHAI CHANGZHENG HOSPITAL
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