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Serratia X-45 for improving abundance of indigofera pseudotinctoria probiotic microorganisms and application of

A probiotic microorganism, X-45 technology, applied in the field of microorganisms to achieve the effect of promoting nitrogen fixation, promoting growth and increasing the total weight of root nodules

Active Publication Date: 2021-11-19
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few domestic reports on screening suitable root nodule growth-promoting strains for different plants

Method used

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  • Serratia X-45 for improving abundance of indigofera pseudotinctoria probiotic microorganisms and application of
  • Serratia X-45 for improving abundance of indigofera pseudotinctoria probiotic microorganisms and application of
  • Serratia X-45 for improving abundance of indigofera pseudotinctoria probiotic microorganisms and application of

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1 sample source

[0034] Strains were screened from soil samples from a bare rock slope located on Yueyang Avenue, Yueyang City, Hunan Province, China. Rock and soil samples were collected from the upper, middle, and lower parts of the slope and brought back to the laboratory for processing. The rocks were used for mineral composition analysis and subsequent tests, and the soil was used for the isolation of bacterial strains. According to the results of mineral analysis, the main components of the rock samples include the following: K 2 O3.71%, Na 2 O 1.39%, CaO 0.21%, MgO 1.28%, P 2 o 5 0.11%, Fe 2 o 3 6.81%, Al 2 o 3 15.21%, MnO 0.04%.

[0035] 2 Separation and screening

[0036] 2.1 Medium

[0037] (1) Strain isolation medium: NaCl 0.3g, KCl 0.3g, (NH 4 ) SO 2 0.5g, MgSO 4 ·7H 2 O 0.3g, FeSO 4 ·7H 2 O 0.03g, MnSO 4 4H 2 O 0.3g, Ca 3 (PO 4 )2 5.0g, sucrose 10g, agar 15-20g, deionized water 1000mL, pH 7.0-7.5.

[0038] (2) Beef extract-peptone m...

Embodiment 2

[0057] Investigate the effect of bacterial growth-promoting effect. Combined with the pot experiment, by planting horse thorn and observing its growth, the screened strains were tested more comprehensively and closer to practical applications, so as to explore the potential effects that can be exerted in actual environmental restoration and production applications.

[0058] Preparation of bacterial agents containing X-45:

[0059] After the strain was activated, it was inoculated into LB liquid medium and fermented for 3 days, and the OD was measured with an ultraviolet spectrophotometer. 600 , ensure the OD of the bacterial solution by diluting or continuing to ferment 600 The value is in the range of 0.8 to 1.2, and then sealed and stored in a refrigerator at 4°C for use.

[0060] When applying bacteria to potted plants, dilute the stored bacterial solution 100 times, put 60mL diluted bacterial solution into each pot, set up 3 parallels for each treatment, and use sterile ...

Embodiment 3

[0081] Explore the effect of bacterial strains on microbial community composition.

[0082] The collected and sorted horsethorn rhizosphere soil samples were sent to Shanghai Majorbio Bio-pharm Technology Co., Ltd (Shanghai Majorbio Bio-pharm Technology Co., Ltd) for sequencing using the IlluminaMiseq platform.

[0083] Detection of microbial diversity in potting soil by high-throughput sequencing, and analysis of microbial community composition in potting soil. Depend on Figure 7 It can be seen that there was no difference in the microbial community composition at the phylum level between the control group and the potting soil treated with the X-45 strain, mainly Proteobacteria, Actinobacteria and Cyanobacteria. However, there are some differences in the relative abundance of microorganisms among the groups. After the treatment of strain X-45, Proteobacteria increased from 35% to 63%, while Actinobacteria decreased from 21.3% to 8.8%, and the relative abundance of Cyanobact...

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Abstract

The invention discloses serratia X-45 for improving abundance of indigofera pseudotinctoria probiotic microorganisms and application of the serratia X-45, and belongs to the technical field of microorganisms. The serratia X-45 provided by the invention can promote production of indigofera pseudotinctoria plants, promote root nodule proliferation of the plants and improve probiotic microorganisms and a nutritional environment. After the serratia X-45 is applied, potassium, calcium, magnesium and other nutrient elements required by plants in rocks are effectively released, rock dissolution is accelerated, effective nitrogen fixation of the indigofera pseudotinctoria is promoted, nitrogen elements required by growth of the indigofera pseudotinctoria are provided, the symbiotic nitrogen fixation capacity of nodule bacteria and host plants is obviously exerted, the total weight of root nodules and the content of hydrolytic nitrogen are increased, and the symbiotic nitrogen fixation capacity is effectively exerted. Meanwhile, the relative abundance of bradyrhizobium in the soil is improved, and the nutrients of the soil are indirectly improved.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to Serratia X-45 for increasing the abundance of probiotic microorganisms in horsethorn and its application. Background technique [0002] Soil spraying technology is a greening technology that sprays the mixture of soil growth matrix materials and plant seeds evenly and at high pressure on rock and soil slopes. The growth matrix material mixture of this technology is prepared with screened soil bacteria, which is mainly aimed at hard slopes such as rocks, and is a greening technology developed for plants to create a suitable growth environment on hard slopes. As one of the important components of the growth matrix material of this technology, the screened soil bacteria play two roles: one is to respond to the change of soil ecological mechanism and environmental stress, accelerate the erosion of rock mass, and effectively improve the Substrate interface fusion; the second i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/20A01P21/00C09K17/14C05F11/08C05G3/80C12R1/425C09K101/00
CPCA01N63/20C09K17/14C05F11/08C05G3/80C09K2101/00Y02P60/20
Inventor 刘超庄家尧杨皓田琨
Owner NANJING FORESTRY UNIV
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