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Method for rapidly detecting chicken fast and slow feathering phenotypes by multiple PCR system

A fast, slow and phenotypic technology, applied in the fields of molecular biology and chicken genetics and breeding, can solve the problem of insufficient identification of fast and slow feather individuals, and achieve the effect of reducing the false negative rate, simple operation and small workload.

Pending Publication Date: 2021-11-26
CHINA AGRI UNIV
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Problems solved by technology

Therefore, it is not enough to distinguish fast and slow feathers only by whether there is an insertion of ev21

Method used

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  • Method for rapidly detecting chicken fast and slow feathering phenotypes by multiple PCR system
  • Method for rapidly detecting chicken fast and slow feathering phenotypes by multiple PCR system
  • Method for rapidly detecting chicken fast and slow feathering phenotypes by multiple PCR system

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Embodiment 1

[0029] Example 1 Establishment and reliability verification of a method for rapid detection of fast and slow feather phenotypes of chickens with a 3-primer PCR system

[0030] The chicken fast and slow feather locus has been located in the range of 10.38Mb to 11.63Mb (the physical map of chicken genome version 2.1 is 9.86Mb to 11.11Mb) (ICGSC Gallus_gallus-4.0 / galGal4, Nov.2011, UCSC). Elferink et al. (2008) proposed the structure of the K gene for the first time, and believed that the slow feather locus K spanned the PRLR gene and the SPEF2 gene, and had a tandem duplication of about 176 kb. The present invention mainly designs primers for two tandem repeated genes: PRLR gene and SPEF2 gene sequence, and detects and verifies through different individuals.

[0031] (1) Primer design and synthesis

[0032] Specific primers were designed according to the differences in the gene structure of fast and slow feathers, and were synthesized by Shanghai Sangong. (PRLR gene and SPEF2 ...

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Abstract

The invention provides a molecular identification method for chicken fast and slow feathering phenotypes. A primer K-R1: SEQ ID NO.1, a primer K-F1: SEQ ID NO.2 and a primer K-R2: SEQ ID NO.3 which are designed for a PRLR gene and an SPEF2 gene are used for amplification. Compared with a traditional PCR method, the three-primer PCR method has the advantages that the cost is saved, the false negative rate is low, and the judgment is accurate.

Description

technical field [0001] The invention relates to the fields of molecular biology and chicken genetic breeding, in particular to a method for rapidly detecting chicken fast and slow feather phenotypes with a three-primer PCR system. Background technique [0002] In the modern chicken industry, sexing plays an important role. The techniques for male and female identification mainly include anal turning, gold and silver feather color, fast and slow feather speed, and so on. Because the identification of turning the anus requires the identification personnel to master certain techniques, and it will cause harm to the chicks during the identification process, resulting in an increase in the mortality rate of the chicks. Therefore, in order to ensure the economic benefits of the chicken industry, it is particularly important to adopt a sex-separation technology that is harmless to chickens. [0003] In the self-sexing of chickens, the identification of fast and slow feather speed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/686C12N15/11
CPCC12Q1/6888C12Q1/686C12Q2600/16C12Q2600/124C12Q2537/143C12Q2565/125
Inventor 陈思睿吕慧娇屈元启张康宁翟尚昆王涛
Owner CHINA AGRI UNIV
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