Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

myadm-targeted siRNAs and their applications

A targeted and molecular technology, applied in the field of siRNA targeted by MYADM, can solve the problems of poor prognosis, high metastasis and invasion rate in patients with esophageal cancer, and achieve the effect of reducing clone formation ability, inhibiting proliferation and reducing expression

Active Publication Date: 2022-07-01
NANTONG TUMOR HOSPITAL
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Despite considerable diagnostic and therapeutic advances in recent years, the prognosis of patients with esophageal cancer remains poor due to high rates of metastasis and invasion

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • myadm-targeted siRNAs and their applications
  • myadm-targeted siRNAs and their applications
  • myadm-targeted siRNAs and their applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Immunohistochemical staining to obtain the expression of MYADM gene in the tissue of patients with esophageal cancer

[0025] Immunohistochemical staining steps: Take the paraformaldehyde-fixed esophageal cancer tissue, embed it in paraffin, section it serially, and place it on a glass slide; place the immunohistochemical slides in an oven at 80°C for 30 min; Soak in toluene I and xylene II for 15 min each; then put in absolute ethanol, 95% ethanol, 80% ethanol, and 70% ethanol for 2 min each; finally, put the tablet into ultrapure water for immersion and cleaning; put the tablet in EDTA In the antigen retrieval solution, after the pressure cooker is boiled until gas is released, continue to heat for 3 minutes, leave the heat source, open the lid of the pressure cooker and cool; after rinsing 3 times with PBST, use an immunohistochemical pen to circle the tissue, and then use H 2 O 2 Incubate at room temperature for 20 min; dilute the primary antibody with a...

Embodiment 2

[0026] Example 2: Design of MYADM small interfering fragments

[0027] Three siRNA sequences were designed for the MYADM gene and synthesized by Heyuan Biotechnology (Shanghai) Co., Ltd.

[0028] The base sequence of siRNA-1 is as follows,

[0029] Sense sequence: 5'-CGGCGAGAUCACUGGCUAUdTdT-3',

[0030] Antisense sequence: 5'-AUAGCCAGUGAUCUCGCCGdTdT-3';

[0031] The base sequence of siRNA-2 is as follows:

[0032] Sense sequence: 5'-UCUACCAGUUCGAUGAGAAdTdT-3',

[0033] Antisense sequence: 5'-UUCUCAUCGAACUGGUAGA dTdT-3'

[0034] The base sequence of siRNA-3 is as follows:

[0035] Sense sequence: 5'-GGCAACUGGUCCAUGUUCAdTdT-3',

[0036] Antisense sequence: 5'-UGAACAUGGACCAGUUGCCdTdT-3'

Embodiment 3

[0037] Example 3: Screening of MYADM small interfering fragments

[0038] Cell culture conditions and medium: Eca109 cell line was cultured in 5% CO with RPMI 1640 (HyCloneSH30809.01) containing 10% fetal bovine serum 2 , in a 37°C incubator.

[0039] Cell transfection: Dilute siRNA-1, siRNA-2 and siRNA-3 into 20μM solutions according to the instructions respectively; after digesting the cells, spread them in a 6-well plate, and transfect when the cell density reaches 30-50%. Prepare four sterile 1.5mL EP tubes, add 200μL of OPTI-MEM and 5μL of the corresponding siRNA-1, siRNA-2, siRNA-3 and negative control siRNA solutions into the four EP tubes respectively, vortex quickly for 10s It is completely mixed; then add 6 μL of siRNA-Mate transfection reagent to the 4 EP tubes respectively, let stand for 10 min at room temperature to make the siRNA and transfection reagent form a transfection complex, and then add them to the corresponding wells of the 6-well plate, gently. Shake...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses MYADM-targeted siRNA and its application, and belongs to the technical field of molecular biological technology and genetic engineering. The invention provides a small interfering RNA for inhibiting the expression of MYADM gene and its application. By transfecting the small interfering RNA in esophageal cancer cells, it can regulate and interfere with the expression of MYADM gene, reduce the ability of cell clone formation, and inhibit cell wound healing and invasion; therefore Small interfering RNA that inhibits the expression of MYADM gene can effectively inhibit the proliferation, migration and invasion of esophageal cancer cells by reducing the expression of MYADM. The invention provides a new potential drug for the treatment of esophageal cancer, and has high clinical application prospect and value.

Description

technical field [0001] The invention belongs to the technical field of molecular biotechnology and genetic engineering, and more particularly, relates to MYADM-targeted siRNA and its application. Background technique [0002] Esophageal cancer is a major public health problem worldwide and one of the most aggressive malignant tumors. Despite considerable diagnostic and therapeutic advances in recent years, the prognosis of patients with esophageal cancer remains poor due to high rates of metastasis and invasion. Studying the effects of metastasis and invasion-related genes on the proliferation and metastasis of esophageal cancer cells has important theoretical significance and application value for improving the prognosis of patients with esophageal cancer. [0003] RNAi technology can specifically delete or turn off the expression of specific genes. The application of RNAi technology to specifically introduce small interfering RNAs into mammalian and human cells to reduce ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113A61K31/713A61P35/00A61P35/04A61P1/00
CPCC12N15/1135A61K31/713A61P35/00A61P35/04A61P1/00C12N2310/14C12N2320/30
Inventor 杨秋星张金业郭丽媛沈爱国
Owner NANTONG TUMOR HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com