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Universal modification method and application of metal tracheal scaffold material

A technology for tracheal stents and metal stents, which is applied in the interdisciplinary field of biomedical polymer materials and interventional medicine, can solve problems such as difficult processing, insufficient drug loading, and large toxic and side effects, and achieve low price, ability to inhibit in vitro migration, The effect of inhibiting angiogenesis

Pending Publication Date: 2021-09-24
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above technologies have their own advantages, there are also some shortcomings, such as insufficient drug loading, sudden drug release, easy disintegration, large toxic and side effects, and difficult processing in actual production and application.

Method used

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  • Universal modification method and application of metal tracheal scaffold material
  • Universal modification method and application of metal tracheal scaffold material
  • Universal modification method and application of metal tracheal scaffold material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Chitosan was dissolved in 1% HCl solution to prepare a chitosan solution with a mass concentration of 1%. Add 0.2mL hydrogen peroxide to 100mL chitosan solution, mix well, and sonicate at room temperature for 5min. Afterwards, the anode platinum sheet and the cathode metal support were immersed in the above-mentioned 100mL chitosan solution, and the distance between the two poles was set to be 2.5cm. A programmable DC power supply was used to apply a current of 1 mA at both ends of the electrodes, and after 1 hour of action, a chitosan-coated metal stent could be obtained.

[0023] The vasoinhibitory peptide was synthesized by microwave peptide synthesizer, and separated and purified by high performance liquid chromatography. Afterwards, the chitosan-coated metal stent and the vasoinhibitory peptide were labeled with a biotin labeling kit, and then an appropriate amount of streptavidin was added to the reaction system for chemical cross-linking. Finally, a chitosan-fu...

Embodiment 2

[0027] The chitosan-coated stent obtained in Example 1 was rinsed under running water for 1 hour and then photographed with a light microscope.

[0028] figure 2 For the light microscope pictures of the chitosan-coated stents obtained in Example 1 and Example 2, as can be seen from the figure, a uniform and transparent chitosan coating is formed on the surface of the stent.

Embodiment 3

[0030]Escherichia coli (E.coli) and Staphylococcus aureus (S.aureus) were used to evaluate the broad-spectrum antibacterial properties of scaffold materials. OD 600 The value was adjusted to 0.2, and then the scaffold material was added for co-cultivation for 4h. Draw 0.1mL of bacterial solution and dilute step by step for 10 -2 to 10 -7 After doubling, evenly spread on the LB plate, after overnight culture at 37°C, take pictures and count the number of colonies. The antibiotic solution was used as the quality control group, and a blank control group was set up. The antibacterial rate was calculated according to the following formula:

[0031]

[0032] image 3 Chitosan functionalized tracheal scaffold (marked as Modified Scaffold in the legend), metal scaffold (marked as Scaffold in the legend), blank control (marked as B.C. in the legend) co-cultured with Escherichia coli and Staphylococcus aureus Light microscope images, from left to right are blank control group, ...

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Abstract

The invention discloses a universal modification method and application of a metal tracheal scaffold material. The modification method of the metal tracheal scaffold material comprises the following steps: carrying out electro-deposition processing by taking a platinum sheet as an anode, a conductive metal tracheal scaffold as a cathode and a chitosan solution as an electrolyte to prepare a chitosan coated metal tracheal scaffold; respectively marking a chitosan coated metal scaffold and a bioactive protein by adopting a commercial biotinylation kit; and coupling the chitosan coated metal scaffold and the bioactive protein with streptavidin to prepare the novel tracheal scaffold material with controllable biological activity and biocompatibility. The modification method disclosed by the invention can be used as a universal processing strategy to be applied to preparation of the tracheal scaffold material with multiple functions of resisting infection, inhibiting scar formation and vascularization and the like.

Description

technical field [0001] The invention belongs to the intersection field of biomedical polymer materials and interventional medicine, and specifically relates to a general modification method and application of a metal trachea stent material. Background technique [0002] With the development of interventional medicine, the clinical demand for metal tracheal stents is increasing, and it is of great significance to develop new tracheal stents for the treatment of tracheal stenosis. At present, the bare metal stents used clinically are prone to cause granulation tissue hyperplasia at the contact site. The patient needs to undergo a second surgical treatment, and postoperative complications such as massive hemoptysis, tracheal mucosal tear, and tracheal collapse are prone to occur, which seriously jeopardizes the quality of the patient's prognosis. Therefore, it is very important to develop processing technologies for controllably editing the surface physicochemical and biologic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C25D9/02C25D7/00A61L31/16A61L31/10A61F2/04
CPCC25D9/02C25D7/00A61F2/04A61L31/10A61L31/16A61F2002/046A61L2420/02A61L2420/06A61L2300/252A61L2300/404A61F2240/001C08L5/08
Inventor 韩新巍赵亚楠田川刘灶渠
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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