Novel coronavirus three-point mutation S gene identification kit based on high-resolution melting curve and identification method thereof

Abstract

The invention discloses a novel coronavirus three-point mutation S gene identification kit based on a high-resolution melting curve and an identification method thereof, belongs to the field of biological medicines, and is characterized in that amplification primers are designed according to three different mutation sites of novel coronavirus S genes, and S genes with different site mutations are identified through an optimized high-resolution melting curve reaction system. The kit is easy to operate and high in reaction speed, PCR products do not need to be post-processed, operability and reproducibility are achieved, and the kit can be used for identifying the novel coronavirus S gene with different site mutations.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a novel coronavirus point mutation S gene identification kit based on a high-resolution melting curve, a detection method and an application thereof. Background technique [0002] Since the outbreak of the new crown pneumonia epidemic, it has brought huge disasters to human society, and it has not been effectively controlled so far. The rapid spread of the new coronavirus has not only seriously affected the development of global public health, but also put forward higher requirements for port health quarantine and epidemic prevention and control. Genetic mutations are a common phenomenon in the natural evolution of viruses, and studies have shown that novel coronavirus mutants may make them more transmissible, or have the ability to evade certain antibody immune responses. The spike protein (S) on the surface of the new coronavirus plays a very important role in ...

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Problems solved by technology

However, these identification methods have shortcomings: although SSCP has high sensitivity, it can only distinguish between normal strands and mutant strands by electrophoresis. To determine the mutation type and mutant gene locus, further sequencing is still required, and the electrophoresis conditions are relatively strict; High-performance liquid chromatography can only check whether there is a mutation, but cannot detect the type of mutation, and the judgment of the result is error-prone; sequencing technology is widely used, but it is time-consuming and expensive, and it is not suitable for the requirements of rapid detection at ports

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