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Modified sealing agent and preparation method thereof

A technology of blocking agent and modifying agent, which is applied in the field of modified blocking agent and its preparation, can solve the problems of false negative stability, incomplete blocking, and affecting sensitivity, etc., and achieve improved stability, good dispersibility, and reduced interprotein The effect of reunion

Pending Publication Date: 2021-12-03
广西康柏莱科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, many liquid reagents still have the problem of incomplete sealing and affecting the sensitivity. At the same time, the reagents with incomplete sealing have problems of false positive or false negative and poor stability in clinical practice.

Method used

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  • Modified sealing agent and preparation method thereof
  • Modified sealing agent and preparation method thereof
  • Modified sealing agent and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0022] A modified sealing agent, comprising the following raw materials in parts by weight: 15 parts of sheep serum albumin, 5 parts of ovalbumin, 6 parts of gelatin, 50 parts of modifiers, 80 parts of modifiers, and 100 parts of chondroitin sulfate; The active agent is a combination of p-dodecylbenzenesulfonyl azide and disodium dithiobutamate according to the mass ratio of 4:1; the modifier is cysteine ​​desulfurase and biotin according to the mass ratio of 1:7 The combination;

[0023] (1) adding water to sheep serum albumin and ovalbumin for dilution, then adding gelatin therein and stirring to mix evenly to obtain protein liquid;

[0024] (2) Add a modifying agent to the protein solution and mix evenly, then place it at a temperature of 37°C and stir for 15 minutes to obtain a modified protein solution, add a modifying agent to the modified protein solution, and continue to stir and react at a temperature of 37°C for 15 minutes to obtain Modifying the protein solution, c...

Embodiment 2

[0027] A modified sealing agent, comprising the following raw materials in parts by weight: 5 parts of sheep serum albumin, 1 part of gelatin, 20 parts of modifiers, 30 parts of modifiers, 50 parts of chondroitin sulfate; The combination of alkylbenzenesulfonyl azide and disodium dithiobutamate according to the mass ratio of 1:5; the modifier is the combination of cysteine ​​desulfurase and biotin according to the mass ratio of 10:1;

[0028] (1) adding water to sheep serum albumin and ovalbumin for dilution, then adding gelatin therein and stirring to mix evenly to obtain protein liquid;

[0029] (2) Add a modifying agent to the protein solution and mix evenly, then place it at a temperature of 36°C and stir for 12 minutes to obtain a modified protein solution, add a modifying agent to the modified protein solution, and continue to stir and react at a temperature of 36°C for 12 minutes to obtain Modifying the protein solution, centrifuging the modified protein solution to sep...

Embodiment 3

[0032] A modified sealing agent, comprising the following raw materials in parts by weight: 8 parts of sheep serum albumin, 1 part of ovalbumin, 2 parts of gelatin, 30 parts of modifiers, 50 parts of modifiers, 60 parts of chondroitin sulfate; The active agent is a combination of p-dodecylbenzenesulfonyl azide and disodium dithiobutamate according to the mass ratio of 2:1; the modifier is cysteine ​​desulfurase and biotin according to the mass ratio of 1:4 The combination;

[0033] (1) adding water to sheep serum albumin and ovalbumin for dilution, then adding gelatin therein and stirring to mix evenly to obtain protein liquid;

[0034] (2) Add a modifying agent to the protein solution and mix evenly, then place it at a temperature of 37°C and stir for 10 minutes to obtain a modified protein solution, add a modifying agent to the modified protein solution, and continue to stir and react at a temperature of 37°C for 10 minutes to obtain Modifying the protein solution, centrifu...

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Abstract

The invention provides a modified sealing agent and a preparation method thereof, and belongs to the technical field of medical products, the modified sealing agent comprises the following raw materials by weight: 5-15 parts of sheep serum protein, 0-5 parts of ovalbumin, 1-6 parts of gelatin, 20-50 parts of a modifier, 30-80 parts of a modifier, and 50-100 parts of chondroitin sulfate. The surface of the obtained modified sealing agent has uniform charges, so that when the modified sealing agent is used for sealing, the sealing effect is good, the reagent has better dispersity, the agglomeration condition among proteins is reduced and the stability and the preservation time of the reagent are improved.

Description

technical field [0001] The invention belongs to the field of medical products and technologies, and in particular relates to a modified sealing agent and a preparation method thereof. Background technique [0002] Immunoturbidimetric analysis is an analytical technique that combines the precipitation reaction in the liquid phase with modern optical instruments and automatic analysis techniques. When the antibody concentration is fixed, the amount of immune complexes formed increases with the amount of antigen in the test sample. As the concentration increases, the turbidity of the reaction solution also increases. By comparing the turbidity of the reaction solution with a series of standards, the content of the antigen in the test sample can be calculated. This technology has been widely used in medical testing. [0003] The solid-phase adsorption protein is non-specific. After the target immune protein is coated, the solid-phase surface of the unadsorbed protein still has ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543
CPCG01N33/54393Y02P20/55
Inventor 李名星张设熙莫智林韦佳志韦松利方运清
Owner 广西康柏莱科技有限公司
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