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Application of metformin in preparation of medicine for treating hand-foot-and-mouth disease

A technology for metformin and hand, foot and mouth disease, which is applied in the directions of pharmaceutical formulations, antiviral agents, and medical preparations containing active ingredients, and can solve problems such as the lack of metformin application.

Active Publication Date: 2021-12-07
MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, it has been reported in the literature that metformin has the effect of inhibiting a variety of viruses, including human immunodeficiency virus (HIV), hepatitis B virus (HBV), hepatitis C virus (HCV), human papillomavirus (HPV), influenza virus, Ke Saatchi virus B3, etc., but there is no application of metformin in the literature against EV71 and CVA16 that cause hand, foot and mouth disease

Method used

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  • Application of metformin in preparation of medicine for treating hand-foot-and-mouth disease
  • Application of metformin in preparation of medicine for treating hand-foot-and-mouth disease
  • Application of metformin in preparation of medicine for treating hand-foot-and-mouth disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1 2

[0036] Example 1 Inhibitory Effect of Metformin on EV71 and CVA16 Replication

[0037] 1. Cell Culture

[0038] Culture medium for subculture of HCT-8 cells: DMEM (Gibco) culture medium containing 10% fetal bovine serum (Gbico), penicillin and streptomycin double antibody 100 U / ml (Gibco).

[0039] The culture medium used for HCT-8 cell dilution virus: DMEM culture medium containing penicillin and streptomycin double antibody 100U / ml (Gibco).

[0040] The culture medium used for HCT-8 cell dilution drug: DMEM culture medium containing 2% fetal bovine serum (Gbico), penicillin and streptomycin double antibody 100U / ml (Gibco).

[0041]When the confluence of HCT-8 cells reaches 90%, add 0.25% trypsin-EDTA (Gibco) to the culture flask, digest at 37°C for 2 minutes, discard the trypsin, add complete culture medium to blow off, passage at 1:3, 1- Passage once every 2 days.

[0042] 2. Cytotoxicity detection

[0043] HCT-8 cells were seeded in 96-well plates, 3×10 4 pc / well, 37℃...

Embodiment 2 2

[0072] Example 2 The inactivation effect of metformin on EV71 and CVA16

[0073] Vero cells were seeded in 6-well plates, 9×10 5 pc / well, 37℃, 5%CO2 After 24 hours, the medium in the well plate was discarded and added at 37°C, 5% CO 2 Cells were infected with EV71 or CVA16 for 1 h after co-incubating with metformin or DMSO for 1 h. After 1 hour, the virus liquid was discarded and the culture medium used for diluting the drug was added, and the virus liquid was incubated at 37°C and 5% CO. 2 CPE was observed and recorded after being cultured in medium for 72 hours, and half of the tissue cell infection amount of the virus (50% tissue culture infectious doses, TCID) was calculated according to the CPE results. 50 ).

[0074] from Figure 5 It can be seen that metformin has no direct inactivation effect on EV71 and CVA16.

Embodiment 3 2

[0075] Example 3 Effect of metformin on EV71 and CVA16 receptor SCARB2 protein content

[0076] HCT-8 cells were seeded in 6-well plates, 9×10 5 pc / well, 37℃, 5%CO 2 After 24 hours, the medium in the well plate was discarded, and different concentrations of metformin were added. A normal control group (the culture fluid used for diluting the drug) and a metformin drug group (20mM, 5mM and 1.25mM) were set. 24 hours after the addition of the drug, the total cell protein was extracted and detected by western blot, which was the same as in Example 1, wherein the SCARB2 antibody was a product of Abcam.

[0077] from Image 6 As can be seen, metformin dose-dependently downregulated the protein content of EV71 and CVA16 receptor SCARB2.

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Abstract

The invention provides application of metformin in preparation of a medicine for treating the hand-foot-and-mouth disease. The metformin provided by the invention has an obvious effect of resisting replication of enterovirus 71 type (EV71) and coxsackie virus A16 type (CVA16) causing the hand-foot-and-mouth disease, the antiviral effect is different from the existing clinical application of the metformin, and an application prospect is wide.

Description

technical field [0001] The invention belongs to the field of medical applications, in particular to the application of metformin in the preparation of medicines for treating hand, foot and mouth disease. Background technique [0002] Enterovirus 71 (EV71) and Coxsackievirus A16 (CVA16) are both open-loop single positive-strand RNA viruses, both of which belong to the genus Enterovirus in the family Picornaviridae. ), which can cause serious social harm. [0003] In December 2015, the State Food and Drug Administration approved the production registration application for the production registration of Enterovirus 71 Inactivated Vaccine, a Class 1 new drug for preventive biological products developed by Beijing Kexing Biological Products Co., Ltd. and the Institute of Medical Biology, Chinese Academy of Medical Sciences , the vaccine was officially launched in the first half of 2016. According to China's statutory infectious disease report, since the vaccine was launched, th...

Claims

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Application Information

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IPC IPC(8): A61K31/155A61P31/14
CPCA61K31/155A61P31/14
Inventor 李玉环王辉强蒋建东
Owner MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
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