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Monoclonal antibody for detecting new coronavirus

A technology of virus detection and antibody, applied in the field of monoclonal antibody for detection of new coronavirus, to improve the accuracy and solve the effect of missed diagnosis

Pending Publication Date: 2021-12-07
苏州博特龙免疫技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Antibody detection can make up for the shortcomings of nucleic acid detection that is easy to cause missed diagnosis, and has become an important means of detection of new coronavirus.

Method used

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  • Monoclonal antibody for detecting new coronavirus
  • Monoclonal antibody for detecting new coronavirus
  • Monoclonal antibody for detecting new coronavirus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] A monoclonal antibody for detection of new coronavirus, the formula of the monoclonal antibody for detection of new coronavirus is as follows: by weight: prepare ascites, antibody 3mg purified by proteinG; prepare ascites, antibody 1.5mg purified by antigen affinity; prepare ascites, sulfuric acid Ammonium precipitation purified antibody 3 mg; cell supernatant expanded culture, proteinG purified antibody 3 mg; cell supernatant expanded culture, antigen affinity purified antibody 1.5 mg.

[0061] A method for preparing a monoclonal antibody for novel coronavirus detection, comprising the steps of:

[0062] S1. Order mice;

[0063] S2. Mouse immunization procedure: Inject the antigen twice, and use the ELISA method to detect the titer of the mouse serum. The immunization procedure includes the following steps:

[0064] S201. Antigen preparation method: according to the immunization of about 20ug per mouse, the antigen is mixed with the same volume of QuickAntibody-Mouse5...

Embodiment 2

[0100] A monoclonal antibody for detection of new coronavirus, the formula of the monoclonal antibody for detection of new coronavirus is as follows: by weight: prepare ascites, antibody 2mg purified by proteinG; prepare ascites, antibody 1mg purified by antigen affinity; prepare ascites, ammonium sulfate Precipitate purified antibody 2 mg; cell supernatant expanded culture, proteinG purified antibody 2 mg; cell supernatant expanded culture, antigen affinity purified antibody 1 mg.

[0101] A method for preparing a monoclonal antibody for novel coronavirus detection, comprising the steps of:

[0102] S1. Order mice;

[0103] S2. Mouse immunization procedure: Inject the antigen twice, and use the ELISA method to detect the titer of the mouse serum. The immunization procedure includes the following steps:

[0104] S201. Antigen preparation method: according to the immunization of about 20ug per mouse, the antigen is mixed with the same volume of QuickAntibody-Mouse5W;

[0105]...

Embodiment 3

[0140] A monoclonal antibody for detection of new coronavirus, the formula of the monoclonal antibody for detection of new coronavirus is as follows: by weight: prepare ascites, antibody 1mg purified by proteinG; prepare ascites, antibody 0.5mg purified by antigen affinity; prepare ascites, sulfuric acid Ammonium precipitation purified antibody 1mg; cell supernatant expanded culture, proteinG purified antibody 1mg; cell supernatant expanded culture, antigen affinity purified antibody 0.5mg.

[0141] A method for preparing a monoclonal antibody for novel coronavirus detection, comprising the steps of:

[0142] S1. Order mice;

[0143] S2. Mouse immunization procedure: Inject the antigen twice, and use the ELISA method to detect the titer of the mouse serum. The immunization procedure includes the following steps:

[0144] S201. Antigen preparation method: according to the immunization of about 20ug per mouse, the antigen is mixed with the same volume of QuickAntibody-Mouse5W; ...

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Abstract

The invention discloses a monoclonal antibody for detecting a new coronavirus. The formula of the monoclonal antibody for detecting the new coronavirus comprises the following components by weight: 1-3mg of antibody purified by protein G for ascetic fluid preparation, 0.5-1.5 mg of an antibody purified by antigen for ascetic fluid preparation, 1-3mg of an antibody purified by ammonium sulfate precipitation for ascetic fluid preparation, 1-3mg of an antibody purified by protein G for cell supernatant enlarge cultivation, 0.5-1.5 mg of an antibody purified by antigen for cell supernatant enlarge cultivation; the monoclonal antibody for detecting the new coronavirus comprises the following steps of S1, mouse ordering, S2, mouse immunization procedure, S3, fusion, S4, fusion detection and S5, monoclone; the monoclonal antibody for detecting the new coronavirus can effectively detect the new coronavirus, solves the problem of easy missed diagnosis in nucleic acid detection, increases IgM and IgG antibody detection when the nucleic acid detection is negative, can be used as a supplementary means for nucleic acid diagnosis, and greatly improves the accuracy of the new coronavirus detection.

Description

technical field [0001] The invention belongs to the field of biotechnology, and more specifically relates to a monoclonal antibody for detection of novel coronavirus. Background technique [0002] The detection methods of the new coronavirus mainly include nucleic acid detection, antibody detection, and antigen detection. Due to the low detection rate of antigen detection, the current detection of the new coronavirus mainly focuses on antibody and nucleic acid detection. [0003] Nucleic acid detection has high requirements for detection equipment or platforms, high-sensitivity RT-PCR instruments are expensive, and have high requirements for laboratory cleanliness and operators. In addition, nucleic acid detection takes a long time, considering sample transportation, sample In the case of a backlog, the results are usually reported within 24 hours at the fastest. Compared with nucleic acid testing, blood samples for antibody testing are easier to obtain and the quality of th...

Claims

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Application Information

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IPC IPC(8): C07K16/10C12N5/20G01N33/569
CPCC07K16/10G01N33/56983G01N2333/165
Inventor 刘立成吴刚闫广为张治业
Owner 苏州博特龙免疫技术有限公司
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