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Descurainia sophia(L.) Webb. ex Prantl. and/or Lepidium apetalum Willd. formula granule characteristic chromatogram and construction method and identification method thereof

A technology of formula granules and characteristic maps, which is applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve the problem that the formula granules cannot be effectively identified, the separation degree of characteristic peaks is poor, and the determination time of the characteristic maps of the medicinal materials of Tinglizi is long. and other problems, to achieve the effect of shortening detection time, balanced peak area, and easy positioning

Active Publication Date: 2021-12-07
BEIJING KANGRENTANG PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006]Therefore, the technical problem to be solved in the present invention is to overcome the long measurement time and poor separation of characteristic peaks of Tinglizi medicinal materials in the prior art, and the inability to analyze Tinglizi formula granules have shortcomings such as effective identification, thereby providing a characteristic map of Tinglizi formula granules and its construction method and identification method.

Method used

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  • Descurainia sophia(L.) Webb. ex Prantl. and/or Lepidium apetalum Willd. formula granule characteristic chromatogram and construction method and identification method thereof
  • Descurainia sophia(L.) Webb. ex Prantl. and/or Lepidium apetalum Willd. formula granule characteristic chromatogram and construction method and identification method thereof
  • Descurainia sophia(L.) Webb. ex Prantl. and/or Lepidium apetalum Willd. formula granule characteristic chromatogram and construction method and identification method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] This embodiment provides a method for constructing a characteristic map of Tinglizi and / or Tinglizi formula particles, including the following steps,

[0060] Preparation of the Fructus Tingli reference substance solution: take 1.0 g of the Fructus Tingli reference medicinal material, accurately weigh it, put it in a stoppered Erlenmeyer flask, add 25 ml of 70% methanol precisely, seal it tightly, weigh it, and ultrasonically treat it for 30 minutes ( power 250W, frequency 40kHz), take it out, let it cool, weigh again, make up the lost weight with 70% methanol, shake well, filter, take the subsequent filtrate to obtain the Tinglizi reference substance solution.

[0061] Preparation of Tinglizi reference substance solution: take 1.0 g of Tinglizi reference material, accurately weigh it, put it in a stoppered conical flask, accurately add 25ml of 70% methanol, seal it tightly, weigh it, and ultrasonically treat it for 30 minutes ( power 250W, frequency 40kHz), take it out...

experiment example 1

[0070] Experimental Example 1 Methodological Investigation

[0071] 1.1 Precision experiment

[0072] 1.1.1 Repeatability

[0073] Take 6 parts of Tinglizi formula granules (S1), measure according to the method in Example 1, obtain its characteristic spectrum, and use peak No. 2 as a reference peak to calculate its relative peak area and relative retention time. And calculate the RSD. The results are shown in Table 1 and Table 2.

[0074] Table 1 Retention time and relative retention time table of characteristic map repeatability investigation

[0075]

[0076] Table 2 The peak area and relative peak area of ​​the characteristic spectrum repeatability investigation

[0077]

[0078]

[0079] According to the repeatability investigation results, the relative retention time RSD of each characteristic peak is 0%-0.3%, and the relative peak area RSD is in the range of 0%-1.7%, indicating that the characteristic spectrum has good repeatability.

[0080] 1.1.2 Intermed...

experiment example 2

[0096] Experimental Example 2 Methodological Investigation

[0097] 2.1 Precision experiment

[0098] 2.1.1 Repeatability

[0099] Take 6 parts of Tinglizi formula granules (S18), and measure them according to the method in Example 1 to obtain their characteristic spectrum. Taking peak No. 6 as the reference peak, calculate the relative peak area and relative retention time, and calculate the RSD. The results are shown in Table 7 and Table 8.

[0100] Table 7 Retention time and relative retention time table of characteristic map repeatability investigation

[0101]

[0102] Table 8 Characteristic spectrum repeatability investigation peak area and relative peak area

[0103]

[0104]

[0105] Through the above experiments, according to the repeatability investigation results, the relative retention time RSD of each characteristic peak is 0%-0.5%, and the relative peak area RSD is in the range of 0%-1.5%, indicating that the repeatability of the characteristic spectr...

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Abstract

The invention belongs to the technical field of traditional Chinese medicine identification, and particularly relates to a Descurainia sophia(L.) Webb. ex Prantl. and / or Lepidium apetalum Willd. formula granule specific chromatogram and a construction method and an identification method thereof. The construction method comprises the following steps: preparing a test solution, and detecting under a specific elution gradient condition to obtain a characteristic chromatogram of Descurainia sophia(L.) Webb. ex Prantl. and / or Lepidium apetalum Willd. formula granules. According to the method, the effective components of the Descurainia sophia(L.) Webb. ex Prantl. formula granules and / or the Lepidium apetalum Willd. formula granules are detected by adopting a high performance liquid chromatography, acetonitrile is used as a mobile phase A, an acid-containing aqueous solution with the volume fraction of 0.1-0.3% is used as a mobile phase B, and the characteristic chromatogram of the Descurainia sophia(L.) Webb. ex Prantl. formula granules and / or the Lepidium apetalum Willd. formula granules under a specific gradient elution procedure is constructed. According to the method, the detection time is greatly shortened, the analysis efficiency is improved, each characteristic peak is completely separated, the peak shape is good, interference is avoided, positioning is easy, the characteristic information of a sample can be comprehensively and accurately reflected, and the Descurainia sophia(L.) Webb. ex Prantl. formula granules and Lepidium apetalum Willd. formula granules are effectively identified.

Description

technical field [0001] The invention belongs to the technical field of identification of traditional Chinese medicines, and in particular relates to a characteristic map of formula granules of Tinglizi and / or Tinglizi and its construction method and identification method. Background technique [0002] Tinglizi is the dry mature seed of Descurainia sophia (L.) Webb, exPrantl. or Lepidium apetalum Willd. of cruciferous plants. The former is commonly called "Nan Ting Li Zi", and the latter is commonly called "Bei Ting Li Zi". This product has the functions of purging the lungs and relieving asthma, promoting water to reduce swelling, and is used for phlegm and saliva obstructing the lungs, wheezing and coughing with excessive phlegm, distension of the chest and flanks, inability to lie on the back, edema of the chest and abdomen, and difficulty in urinating. According to textual research, Tinglizi was first recorded in "Shen Nong's Materia Medica", listed as inferior grade, an...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/54G01N30/74
CPCG01N30/02G01N30/06G01N30/54G01N30/74
Inventor 张志强吉艳慧史国华付静安琪
Owner BEIJING KANGRENTANG PHARMA
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