Application of curcuma zedoary extract in preparation of medicine for treating breast cancer bone metastasis

A technology of Curcuma curcuma extract and breast cancer, applied in the field of traditional Chinese medicine, can solve problems such as reports on the function of meso-Hannokinol, and achieve the effects of inhibiting bone metastasis and reducing bone damage

Active Publication Date: 2021-12-14
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] It is a natural product isolated from the Chinese herbal medicine Zezhu, and there is no report on the function of meso-Hannokinol

Method used

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  • Application of curcuma zedoary extract in preparation of medicine for treating breast cancer bone metastasis
  • Application of curcuma zedoary extract in preparation of medicine for treating breast cancer bone metastasis
  • Application of curcuma zedoary extract in preparation of medicine for treating breast cancer bone metastasis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 meso-Hannokinol inhibited the metastasis of breast cancer cells with high bone metastasis MDA-MB-231BO in the scratch test

[0021] Take the MDA-MB-231BO cells in the logarithmic growth phase, digest and centrifuge with trypsin, resuspend the cells in complete medium, and adjust the cell number to 3×10 with a cell counter. 5 cells / ml, inoculated into 6-well plates, 2ml per well, and cultured in an incubator. Use a 200 microliter pipette tip to draw a vertical scratch on the cell surface, then wash the cells twice with PBS to remove the scratched residual cells, add different concentrations of meso-Hannokinol serum-free medium for culture, Continue culturing for 24 hours until the cells adhere to the wall, observe the changes of cell migration after drug treatment at 0h and 24h under a microscope and take pictures, and calculate the scratch healing rate=(0h scratch width-24h scratch width) / 0h scratch width×100 %, which reflects the cell migration ability.

[...

Embodiment 2

[0023] Example 2 meso-Hannokinol inhibited the metastasis of breast cancer cells with high bone metastasis MDA-MB-231BO in Transwell experiment

[0024] MDA-MB-231BO cells were spread into the upper chamber of the transwell plate at 30,000 / well, and cultured in serum-free medium containing different concentrations of meso-Hannokinol, and culture medium containing 10% serum was added to the lower chamber of the transwell plate The base is used to induce the migration of tumor cells. After culturing for 24 hours, take out the small chamber and wash it twice with PBS, put the small chamber in paraformaldehyde fixative solution for 30 minutes, wash both sides with PBS again, and put the small chamber in crystal violet Stain the cells in the staining solution for 30 minutes, wash with PBS again, and gently wipe off the non-migrated cells on the upper layer of the chamber membrane with a cotton swab, photograph the migrating cells on the lower layer of the chamber membrane under a mi...

Embodiment 3

[0026] Example 3 Meso-Hannokinol inhibits the osteoclast differentiation of macrophage RAW264.7 cells induced by the conditioned medium of breast cancer cells with high bone metastasis MDA-MB-231BO

[0027] The culture medium of breast cancer cells with high bone metastasis MDA-MB-231BO was collected, filtered through a 0.22 μm aqueous phase filter, and mixed with medium containing 10% serum at a ratio of 1:1 to obtain breast cancer cells with high bone metastasis MDA-MB- Conditioned medium (CM) of 231BO. Spread RAW264.7 into a 12-well plate at 100,000 / well, and culture RAW264.7 cells for 48 hours with ordinary medium, conditioned medium, and conditioned medium containing meso-Hannokinol (50 μM) for 48 hours. Tartrate-resistant acid phosphatase detection kit detects the level of tartrate-resistant acid phosphatase (TRAP) in RAW264.7 cells. Tartrate-resistant acid phosphatase, a glycosylated metalloprotease, is considered to be an important indicator of osteoclastic activity i...

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Abstract

The invention discloses an application of a curcuma zedoary extract in preparation of a medicine for treating breast cancer bone metastasis. The invention relates to an application of a curcuma zedoary extract meso-Hannokinol in preparation of a medicine for treating breast cancer metastasis, in particular to an application of the curcuma zedoary extract meso-Hannokinol in preparation of a medicine for treating breast cancer bone metastasis, preferably an application of the curcuma zedoary extract meso-Hannokinol in preparation of a medicine for treating breast cancer bone metastasis. Experiments prove that the curcuma zedoary extract meso-Hannokinol can reduce the migration ability of breast cancer cells so as to prevent bone metastasis; osteolysis and bone destruction caused by breast cancer can be reduced to reduce bone injury caused by breast cancer bone metastasis; and the curcuma zedoary extract meso-Hannokinol can effectively inhibit bone metastasis of breast cancer cells in nude mice, so that the curcuma zedoary extract meso-Hannokinol can be used for developing related drugs for resisting breast cancer bone metastasis.

Description

technical field [0001] The invention relates to traditional Chinese medicine technology, in particular to the application of zedoary curcuma extract in the preparation of medicine for bone metastasis of breast cancer. Background technique [0002] Breast cancer is one of the most common malignant tumors in women in the world, and about 70% of patients metastasize at the advanced stage of cancer. Breast cancer metastasis is organ-selective. Bone tissue is the most likely site for metastasis. The proportion of metastasis far exceeds that of liver, lung, kidney and other organs. About 70% of the metastasis is bone metastasis, thoracic spine, lumbar spine, ribs and hip joints, etc. Both are common sites of bone metastasis. Once bone metastasis occurs, the 5-year survival rate of patients is only 20%-30%. Bone metastases from breast cancer often cause a series of bone-related diseases such as intractable bone pain, hypercalcemia, pathological fractures, and dysfunction, which se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/05A61K36/9066A61P35/04
CPCA61K31/05A61K36/9066A61P35/04Y02A50/30
Inventor 杨蕾孔令义朱袁
Owner CHINA PHARM UNIV
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