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Acid-producing bacterium and acid-producing bacterium agent for treating manure of livestock and poultry and application of acid-producing bacterium and acid-producing bacterium agent

A technology of acid-producing bacteria and livestock and poultry manure, applied in the field of microorganisms, can solve the problems of difficult promotion of biogas technology, insufficient methane production, and long start-up time of anaerobic digestion reactors

Active Publication Date: 2021-12-14
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the temperature is lower than 20°C, or even below 10°C, the key microorganisms in the anaerobic digestion process, such as acid-producing bacteria such as Anaerolineaceae and Erysipelothrix, and methanogens such as Methanosaeta and Methanosarcina, have a reduced growth and metabolism capacity, which leads to anaerobic digestion reactor Long start-up time and insufficient methane production make it difficult to promote biogas technology

Method used

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  • Acid-producing bacterium and acid-producing bacterium agent for treating manure of livestock and poultry and application of acid-producing bacterium and acid-producing bacterium agent
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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Application of embodiment 1 low-temperature acid-producing bacteria agent in anaerobic digestion

[0028] Adjust the solid content of the culture manure to be 4wt%, add bottom mud to the reactor, and inoculate the volume to be 10% of the volume of the biogas slurry, and add bacterial agents to the biogas slurry with an inoculation amount of 10% (v / v), as The treatment group was treated statically in an anaerobic reactor at 10°C for 40 days, and the manure from dairy farms without bacterial solution was also used as a control group for anaerobic digestion to measure the amount of methane produced.

[0029] After 20 days, compared with the control group, the acid production increased by 44%, and the gas production increased by 77%; after 40 days, compared with the control group, the acid production increased by 133%, and the gas production increased by 59%.

[0030] Wherein, the preparation method of the bacterial agent is as follows: a single colony is inoculated in the ...

Embodiment 2

[0032] Embodiment 2, the application of low-temperature acid-producing bacteria agent in anaerobic digestion

[0033] Adjust the solid content of the culture manure to be 4wt%, add bottom mud to the reactor, inoculate the volume as 10% of the volume of the biogas slurry, and add bacterial agents to the biogas slurry with an inoculation amount of 20% (v / v), as The treatment group was statically treated in an anaerobic reactor at 15°C for 40 days, and the dairy farm manure without adding bacterial liquid was also used as a control group for anaerobic digestion to measure the amount of methane produced.

[0034] After 20 days, compared with the control group, the acid production increased by 78%, and the gas production increased by 102%. After 40 days, compared with the control group, the acid production increased by 144%, and the gas production increased by 72%.

Embodiment 3

[0035] Embodiment 3, the application of low-temperature acid-producing bacteria agent in anaerobic digestion

[0036] Adjust the solid content of the culture manure to be 4wt%, add bottom mud to the reactor, and inoculate the volume to be 10% of the volume of the biogas slurry, and add bacterial agents to the biogas slurry with an inoculation amount of 10% (v / v), as The treatment group was statically treated in an anaerobic reactor at 15°C for 40 days, and the dairy farm manure without adding bacterial liquid was also used as a control group for anaerobic digestion to measure the amount of methane produced.

[0037] After 20 days, compared with the control group, the acid production increased by 48%, and the gas production increased by 81%. After 40 days, compared with the control group, the acid production increased by 135%, and the gas production increased by 53%.

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Abstract

The invention relates to the technical field of microbes and particularly relates to an acid-producing bacterium and acid-producing bacterium agent for treating manure of livestock and poultry and application of the acid-producing bacterium and acid-producing bacterium agent. The preservation number of the acid-producing bacterium in the China General Microbiological Culture Collection Center is CGMCC NO: 21664, the acid-producing bacterium is Leuconostoc flalax NX13, the low-temperature anaerobic fermentation efficiency can be increased, the acid-producing bacterium is salt-resistant and low-temperature-resistant, acid can be efficiently produced in a low-temperature anaerobic reactor, the anaerobic fermentation starting time is shortened, the gas production efficiency is increased, the popularization of a biogas anaerobic fermentation technology is facilitated, the resource-converting utilization of the livestock and poultry manure is promoted, and the market prospect is wide.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to an acid-producing bacterium for treating livestock and poultry manure, an acid-producing bacterium agent and applications thereof. Background technique [0002] Leuconostoc is a kind of heterofermentative Gram-positive lactic acid bacteria, and it is the microorganism that plays a major role in the fermentation process of kimchi. The living environment of Leuconostoc is relatively broad, and mainly exists on the surface and roots of plants. Fermentation can metabolize and produce various aromatic compounds such as organic acids, alcohols, and diacetyl, which can regulate intestinal balance, promote nutrient absorption, and improve product flavor. At the same time, it has anti-oxidation and anti-bacterial ability, so it is widely used in the food industry, especially in the production of probiotic products. [0003] In recent years, the large-scale and intensive livestock ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P7/40C02F11/04C12R1/01C02F103/20
CPCC12N1/20C12P7/40C02F11/04C02F2103/20Y02E50/30
Inventor 孙英马艳雯袁博张月杜贤陈俊豪王婷婷张轶欣冯绍轩
Owner CHINA AGRI UNIV