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Culture method for promoting synchronization of thunberg fritillary bulb

A cultivation method, the technology of fritillary fritillaria, applied in the field of promoting the synchronization of fritillary fritillaria bulbs, can solve the problems of slow growth rate and low synchronization rate, reduce production cost, shorten production cycle, and shorten planting time Effect

Pending Publication Date: 2021-12-17
ZHEJIANG WANLI UNIV +1
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Problems solved by technology

[0004] The present disclosure provides a culture method for promoting the synchronization of fritillary bulbs, aiming to solve the problems of low synchronization rate and slow growth rate in existing culture methods

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  • Culture method for promoting synchronization of thunberg fritillary bulb
  • Culture method for promoting synchronization of thunberg fritillary bulb
  • Culture method for promoting synchronization of thunberg fritillary bulb

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Embodiment Construction

[0021] The following will clearly and completely describe the technical solutions in the embodiments of the present disclosure with reference to the drawings in the embodiments of the present disclosure. It should be understood that the specific embodiments described here are only used to explain the present disclosure, not to limit the present disclosure. Based on the embodiments of the present disclosure, all other embodiments obtained by those skilled in the art without creative efforts shall fall within the protection scope of the present disclosure.

[0022] The present embodiment provides a kind of culture method that promotes the synchronization of Fritillaria fritillaria bulbs, comprising the following steps:

[0023] (1) Adventitious bud induction: Cut the petiole of Fritillaria japonica seedlings into 0.5-1cm segments, inoculate them with MS+0.5-1.0mg / L 6-BA+0.1-0.3mg / L NAA+50g / L sucrose+ In the medium of 7g / L agar, induce adventitious buds;

[0024] (2) Seedling c...

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Abstract

The invention provides a culture method for promoting synchronization of thunberg fritillary bulb. The method aims to solve the problems of low synchronization rate and slow growth speed of an existing culture method. The culture method for promoting synchronization of the thunberg fritillary bulb comprises the following steps of (1) adventitious bud induction: cutting a thunberg fritillary bulb tissue culture seedling petiole into small sections of 0.5-1cm, inoculating the small sections into a culture medium, and inducing adventitious buds; (2) seedling culture: after the adventitious buds are induced for 40 days, the bud height is 1-2 cm, longitudinally cutting multiple shoots into small blocks with buds from the base parts, transferring the small blocks into the culture medium, and promoting the buds to grow into seedlings; (3) rooting culture: after adult seedlings are cultured, cutting the adult seedlings with the height of 5cm into single plants, and transferring the single plants into the culture medium for rooting culture; and (4) transplanting: after 3-8 adventitious roots with the length of 1-2cm grow on the base part of the tissue culture seedling, taking out a rooted tissue culture seedling from a culture bottle, and planting the rooted tissue culture seedling into a substrate. According to the culture method, the time for producing the thunberg fritillary bulb tissue culture seedlings is 115 days, and the culture time can be greatly shortened while bulb synchronization is promoted.

Description

technical field [0001] The disclosure belongs to the technical field of Fritillaria fritillaria tissue culture, and in particular relates to a cultivation method for promoting the synchronization of bulbs of Fritillaria fritillata. Background technique [0002] Fritillaria thunbergii Miq. is a perennial herbaceous plant of the genus Fritillaria in the family Liliaceae. The bulb has the functions of clearing heat, resolving phlegm and relieving cough, detoxifying, dissipating stagnation and eliminating carbuncle. Fritillaria fritillaria is mainly propagated by bulbs, and its reproductive coefficient is at a low level compared with that of other medicinal materials. The growth period is long and the species degenerates, which greatly limits the production efficiency. [0003] Plant tissue culture is not limited by the time of season, and a variety of organs or tissues can be used to establish a regeneration system, so as to achieve the purpose of rapid reproduction. Using pla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 丁楚蔚陈志王忠华
Owner ZHEJIANG WANLI UNIV
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