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Macrophage-derived exosome preparation for resisting inflammation and promoting tissue regeneration and preparation method and application of macrophage-derived exosome preparation

A technology of macrophages and exosomes, applied in bone/connective tissue cells, anti-inflammatory agents, applications, etc., can solve problems such as unfavorable wound healing, achieve good clinical application value, promote angiogenesis, and promote wound healing Effect

Pending Publication Date: 2021-12-17
TIANJIN HOSPITAL
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

Early wound inflammation can protect the wound and is beneficial to wound healing, but once the inflammation persists into chronic inflammation, the wound will turn into a chronic wound, which is not conducive to wound healing. Therefore, regulation and control of pro-inflammatory-anti-inflammatory Balance is important for wound healing

Method used

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  • Macrophage-derived exosome preparation for resisting inflammation and promoting tissue regeneration and preparation method and application of macrophage-derived exosome preparation
  • Macrophage-derived exosome preparation for resisting inflammation and promoting tissue regeneration and preparation method and application of macrophage-derived exosome preparation
  • Macrophage-derived exosome preparation for resisting inflammation and promoting tissue regeneration and preparation method and application of macrophage-derived exosome preparation

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Embodiment Construction

[0035] The present invention will be further described below in conjunction with the accompanying drawings and embodiments. It should be noted that the materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0036] 1. Preparation of M2-Exo

[0037] 1. Purchasing mouse mononuclear macrophage leukemia cells (RAW 264.7) from Wuhan Puruosai Biological Co., Ltd. figure 1 ) to identify M0 macrophages. cells at 1 x 10 5 A density of 75cm 3 Add an appropriate amount of complete medium to the culture flask and place in 5% CO 2 , routinely cultured in a 37°C incubator with saturated humidity, and the cells can be passaged when the degree of confluence is about 80%-90%.

[0038] 2. Activation of M2 macrophages: M0 macrophages were treated with 1×10 5 A density of 75cm 3 In the culture flask, after the cells adhered to the wall, the M0 macrophages were treated with 20ng / mL IL-4 (R&D) for 48h.

[0039] 3. Identific...

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Abstract

The invention discloses a macrophage-derived exosome preparation for resisting inflammation and promoting tissue regeneration and a preparation method and application of the macrophage-derived exosome preparation. The macrophage-derived exosome preparation comprises an M2 type macrophage source exosome. The macrophage-derived exosome preparation can be used for preparing medicines, health-care products or nutritional supplements for treating inflammatory diseases, tissue repair and degenerative diseases. The M2 macrophage exosome preparation is simple to prepare, wide in material source and convenient to store, compared with cell therapy, the exosome is small in side effect and not prone to causing immune response, can regulate inflammation balance, promote wound healing and slow down the progression of degenerative diseases after being applied, can be applied in various forms and has good clinical application value.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a macrophage-derived exosome preparation for anti-inflammation and tissue regeneration promotion and its preparation method and application. Background technique [0002] Maintaining skin integrity is crucial to the body, and tissue damage and repair are extremely complex processes in the human body, involving various molecular and genetic epigenetic mechanisms. So far, studies have demonstrated that wound healing occurs through four precise and highly programmed phases: hemostasis, inflammation, proliferation, and remodeling. Disturbance of these phases and failure to occur in a timely and appropriate manner can result in delayed or pathological wound healing. Among them, inflammation is biologically defined as "the response of the body's immune system to certain stimuli", which is mainly caused by various pathogen infections or tissue damage, and is considered to be a prot...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61K9/06A61K47/36A61K47/10A61P19/02A61P29/00A61P13/12A61P17/00A61P9/10A61P19/08A61P19/04A61P21/00A61P25/00A61P17/02A23L33/10C12N5/0775
CPCA61K35/28A61K9/06A61K47/36A61K47/10A61P19/02A61P29/00A61P13/12A61P17/00A61P9/10A61P19/08A61P19/04A61P21/00A61P25/00A61P17/02A23L33/10C12N5/0668A23V2002/00A23V2200/322A23V2200/316A23V2200/318A23V2200/306
Inventor 杨强赵艳红邢逸马信龙窦一鸣杨磊孙逊王敏赵昕
Owner TIANJIN HOSPITAL
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