Itaconic acid derivatives and uses thereof in treating an inflammatory disease or a disease associated with an undesirable immune response
A compound and free technology, applied in the direction of diseases, allergic diseases, diseases transmitted by vectors, etc., can solve the problem of losing the biological effect of DMF
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[1088] Analytical equipment
[1089] NMR spectra were recorded using a Bruker 400MHz Avance III spectrometer equipped with a Bruker 5mm probe or a Bruker 500MHz Avance III HD spectrometer equipped with a Bruker 5mm SmartProbeTM. Unless otherwise stated, spectra are measured at 298K and referenced to solvent resonance. Chemical shifts are reported in parts per million. Data were acquired using BrukerTopSpin software.
[1090] UPLC / MS analysis was performed on a Waters Acquity UPLC system using a Waters Acquity CSH C18 or BEH C18 column (2.1 x 30 mm) maintained at a temperature of 40 °C and using a linear acetonitrile gradient appropriate for the lipophilicity of the compound over 3 or 10 minutes. Dissolve at a constant flow rate of 0.77mL / min. The aqueous portion of the mobile phase was 0.1% formic acid (CSH C18 column) or 10 mM ammonium bicarbonate (BEH C18 column). LC-UV chromatograms were recorded between 210 and 400 nm using a Waters Acquity PDA detector. Mass spectr...
preparation example 1
[1155] According to general procedure 2, using 4-octyl itaconate as itaconate monoester and 3-hydroxypropionitrile as R 2 -OH to prepare Example 1. 1 H NMR (500MHz, DMSO-d6) δ6.25(d, J=1.2Hz, 1H), 5.90(d, J=1.3Hz, 1H), 4.27(t, J=6.0Hz, 2H), 4.02(t ,J=6.6Hz,2H),3.39(s,2H),2.92(t,J=6.0Hz,2H),1.58-1.51(m,2H),1.35-1.21(m,10H),0.89-0.84( m,3H). LCMS m / z 318.2 (M+Na) + (ES + ).
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