Application of PCSK9 inhibitor in preparation of products for promoting hair growth

A technology of inhibitors and products, applied in the field of medicine, can solve problems such as literature, patents and products that have not yet been seen, and achieve the effect of small adverse reactions, good curative effect, and good treatment methods

Active Publication Date: 2022-01-04
陈敏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] So far, there are no relevant literature, patents and products on the application of PCSK9 inhibitors for the treatment of hair loss

Method used

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  • Application of PCSK9 inhibitor in preparation of products for promoting hair growth
  • Application of PCSK9 inhibitor in preparation of products for promoting hair growth
  • Application of PCSK9 inhibitor in preparation of products for promoting hair growth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 The impact of PCSK9 gene knockout on androgenetic alopecia (SA)

[0034] 1. Experimental method

[0035] 1.1 Animal grouping and modeling

[0036] Experimental animals: SPF grade C57BL / 6 (B6) mice, C57BL / 6-PCSK9- / - mice (use CRISPR gene editing technology to knock out Pcsk9 gene exon 2-3, establish Pcsk9 gene knockout mouse model), Mice are derived from Shanghai Model Organisms.

[0037] Mice of different genotypes were divided into three groups: C57BL / 6 mouse negative control group, C57BL / 6 mouse model group and C57BL / 6-PCSK9- / - mouse model group, with 6 mice in each group, 3 mice in each group. The back of each mouse was depilated as an observation area. Except for the negative control group, mice in other groups were subcutaneously injected with testosterone propionate injection [8ml / (kg·d)] once a day for 60 consecutive days to establish the SA model. After continuous subcutaneous injection of testosterone propionate for 30 days, the mice gradually los...

Embodiment 2

[0053] Example 2 Effects of PCSK9 inhibitors on mouse hair growth

[0054] 1. Experimental method

[0055] 1.1 Materials

[0056] Gene 5'-3'Sense 5'-3'Antisense siPCSK9-1 GccuGGAGuuuAuucGGAAdT*dT UUCCgAAuAAACUCcAGGCdT*dT siPCSK9-2 AGGuGuAucuccuAGAcAcdT*dT GUGUCuAGGAGAuAcACCUdT*dT

[0057] Mix equal amounts of siPcsk9-1 and 2 and dilute to 20 μM with normal saline, take the diluted siRNA and mix evenly with lotion.

[0058] PCSK9 interference RNAi inhibitor-2: the RNA sequence is the same as Inclisran from Alnylam Company; PCSK9 interference RNAi inhibitor-3: the RNA sequence is the same as ALN-PCS from Affiris Company.

[0059] (2) PCSK9 small molecule compound inhibitor 1: R-IMPP, a product of Selleck, chemical formula: C 24 h 27 N 3 o 2 , molecular weight: 389.49, structural formula: PCSK9 small molecule compound inhibitor 2: PF-06446846 from Selleck, chemical formula: C 22 h 21 N 7 O, molecular weight: 434.5, structural formula:...

Embodiment 3

[0086] The influence of embodiment 3 various PCSK9 inhibitors on androgenetic alopecia (SA) rat model

[0087] 1. Experimental method

[0088] 1.1 Materials

[0089] Gene 5'-3'Sense 5'-3'Antisense siPCSK9-1 GccuGGAGuuuAuucGGAAdT*dT UUCCgAAuAAACUCcAGGCdT*dT siPCSK9-2 AGGuGuAucuccuAGAcAcdT*dT GUGUCuAGGAGAuAcACCUdT*dT

[0090] Mix equal amounts of siPcsk9-1 and 2 and dilute to 20 μM with normal saline, take the diluted siRNA and mix evenly with lotion. PCSK9 interference RNAi inhibitor-2: the RNA has the same sequence as Inclisran from Alnylam Company; PCSK9 interference RNAi inhibitor-3: the RNA has the same sequence as ALN-PCS from Affiris Company.

[0091] (2) PCSK9 small molecule compound inhibitor 1 (R-IMPP, product of Selleck Company), chemical formula: C 24 h 27 N 3 o 2 , molecular weight: 389.49, structural formula: PCSK9 small molecule compound inhibitor 2 (PF-06446846 from Selleck), chemical formula: C 22 h 21 N 7 O, molecular...

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Abstract

The invention belongs to the technical field of medical biology, and particularly relates to an effect of a proprotein convertase subtilisin/kexin type 9 (PCSK9) in promotion of hair growth and application of a PCSK9 inhibitor in preparation of products for treating alopecia diseases. The PCSK9 inhibitor is characterized in that the PCSK9 inhibitor is a PCSK9 small molecule compound or a PCSK9 interfering RNA or a PCSK9 monoclonal antibody or a PCSK9 mimic peptide or a PCSK9 mimic antibody protein or a PCSK9 antisense oligonucleotide or a PCSK9 vaccine, and the PCSK9 inhibitor is applied to preparation of the products for treating the alopecia diseases. Systematic or external PCSK9 inhibitor products can be further developed and are used for treating the alopecia diseases. The products are remarkable in curative effect and small in adverse reaction.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the effect of PCSK9 in promoting hair growth, and the application of PCSK9 inhibitors in the preparation of products for treating alopecia. Background technique [0002] Alopecia areata (AA) is a non-scarring hair loss with generally normal skin. Usually it is a sudden hair loss patch, which can involve the entire scalp in severe cases. At this time, it is called alopecia totalis (AT). universalis, AU), it is easy to have a serious impact on the appearance and psychology of the patient. Alopecia areata has a longer course of disease, but the prognosis is better. The earlier the intervention, the higher the chance of cure. At present, the etiology is not yet fully understood, and autoimmune dysfunction or instability, neuropsychiatric factors are considered to be important related factors. Many cases had neuropsychiatric trauma before the onset of the disease, such...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K31/7088A61K39/395A61P17/14
CPCA61K45/00A61K31/7088A61P17/14C07K16/40A61K2039/505Y02A50/30
Inventor 陈敏其他发明人请求不公开姓名
Owner 陈敏
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