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Bivalent attenuated vaccine capable of resisting both papaya leaf distortion mosaic virus (PLDMV) and papaya ringspot virus (PRSV)

A papaya and vaccine technology, applied in the direction of viruses, viral peptides, viruses/phages, etc., can solve problems such as diseases, and achieve a significant effect of disease resistance

Active Publication Date: 2022-01-04
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the harm of PLDMV has been increasing year by year, gradually becoming a threat, and PLDMV and PRSV can co-infect papaya, causing more serious diseases
However, there is no report of a bivalent attenuated vaccine against both PRSV and PLDMV for cross-protection

Method used

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  • Bivalent attenuated vaccine capable of resisting both papaya leaf distortion mosaic virus (PLDMV) and papaya ringspot virus (PRSV)
  • Bivalent attenuated vaccine capable of resisting both papaya leaf distortion mosaic virus (PLDMV) and papaya ringspot virus (PRSV)
  • Bivalent attenuated vaccine capable of resisting both papaya leaf distortion mosaic virus (PLDMV) and papaya ringspot virus (PRSV)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0049] 1. Cloning of PRSV Nib (PR) fragment:

[0050] Using the designed primer pair PR-F / R (see the table below for the primer sequence), use the PRSV invasive cloning plasmid pG-H (Tuo Decai, 2011) as a template, and use Prime STAR Max Premix (2×) high-fidelity DNA polymerase (TaKaRa) was amplified to obtain a 330bp NIb gene fragment (PR), which only produced low-abundance vsiRNAs combined with the PLDMV virus silencing suppressor HC-Pro, and was an ideal fragment for targeting viral RNA.

[0051] Table 1 Primer sequences

[0052] Primer name Primer sequence PR-F 5′-CAGTGGTCTCTGTCCAGTCCTGACAAGTTACACGGCAACCTT-3′ PR-R 5′-TGGTCTCAGCAGACCACAAGTGATGACTTCGCTATCTGTGAC-3′

[0053] 2. Construction of bivalent attenuated vaccine pPLDMV-PR vector

[0054] The PR fragment was cloned into PLDMV-E using the Nimble Cloning reaction (the 53rd amino acid K of HC-Pro encoded by the wild-type PLDMV virus was mutated to E (see Tuo D, Zhou P, Zhao G, et al.A doubl...

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Abstract

The invention relates to the technical field of plant protection, in particular to a bivalent attenuated vaccine capable of resisting both a papaya leaf distortion mosaic virus (PLDMV) and a papaya ringspot virus (PRSV). According to the vaccine, PRSV fragments are screened and optimized and are matched with PLDMV attenuated strains, and the vaccine prepared from a formed carrier can resist both the PRSV and the PLDMV, so that cross protection of the PRSV and the PLDMV is realized. Compared with a vaccine resisting PRSV or PLDMV individually, the bivalent attenuated vaccine provided by the invention has a remarkable disease-resistant effect, and a new material and a new way are provided for preventing and controlling the PRSV and the PLDMV through cross protection in the field.

Description

technical field [0001] The invention relates to the technical field of plant protection, in particular to a bivalent attenuated vaccine against both papaya malformed mosaic virus and papaya ringspot virus. Background technique [0002] Papaya is one of the most important tropical economic fruit trees in Hainan, and papaya ringspot virus (PRSV) and papaya leaf distortion mosaic virus (PLDMV) have been the main factors affecting the development of papaya industry. Limiting factor. At present, in terms of cultivation technology, it is still impossible to control ring spot mosaic disease through chemical methods, and the disease-resistant germplasm resources are scarce. [0003] PRSV and PLDMV are both Potyviruses in the family Potyviridae. The disease caused by PRSV can endanger leaves, stems and fruits at the same time. The main symptoms are mottled or deformed leaves, ring spots on the surface of fruits, and water-soaked streaks on stems and petioles, which seriously affect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/40C12N15/84A01G13/00
CPCC07K14/005C12N15/8283A01G13/00C12N2770/34022C12N2770/34034Y02A50/30
Inventor 沈文涛庹德财周鹏言普
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI