Method for screening drug-resistant microorganisms through fluorescence method
A screening method and microbial technology, applied in the direction of microbial-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of overestimation of drug-resistant bacteria, lack of methods for screening drug-resistant bacteria, time-consuming and labor-intensive problems, and achieve improved general Penetration, guaranteed validity, and simplified screening process
Pending Publication Date: 2022-01-07
INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
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Problems solved by technology
However, the pure culture method can only be used to detect cultivable microorganisms, but it is useless for the vast majority (>99%) of non-culturable microorganisms in environmental samples, and it is time-consuming and laborious
Molecular detection technology extracts the total genome of microorganisms, and then detects the presence or absence of resistance genes or quantifies them. This method detects the total resistance genes, including resistance genes carried by dead drug-resistant bacteria and non-expressed resistance genes. resulting in a substantial overestimation of resistant bacteria
[0004] Therefore, the prior art lacks a method that can rapidly screen for drug-resistant bacteria
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[0046]The present invention will be described in detail below in conjunction with specific embodiments. The following examples will help those skilled in the art to further understand the present invention, but do not limit the present invention in any form. It should be noted that those skilled in the art can make several modifications and improvements without departing from the concept of the present invention. These all belong to the protection scope of the present invention.
[0047] In the following, microorganisms are used as examples for illustration. In this case, drug-resistant microorganisms are drug-resistant bacteria, drug-sensitive microorganisms are drug-sensitive bacteria, and microorganism-inhibiting drugs are antibacterial drugs.
[0048] A method for screening drug-resistant microorganisms by fluorescence method, comprising:
[0049] S1: Cultivate the microorganisms for a first predetermined time in a first culture containing antibiotics and HPG (L-type hom...
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The invention relates to a method for screening drug-resistant microorganisms through a fluorescence method. The method comprises the following steps: culturing microorganisms in a first culture containing antibiotics and amino acids of azide or alkynyl groups for a first preset time; transferring the microorganisms cultured by the first culture to a reaction solution of a fluorescent dye containing alkynyl or azide groups to react for a second preset time; and performing fluorescence detection on the microorganisms cultured by the reaction solution, and determining that the microorganisms with fluorescence signals are drug-resistant microorganisms. The invention provides a method for rapidly detecting drug-resistant microorganisms.
Description
technical field [0001] The invention relates to the technical field of biochemical detection and analysis, in particular to a method for screening drug-resistant microorganisms. Background technique [0002] Antibacterial drugs such as antibiotics are one of the greatest inventions in human history. They have effectively controlled bacterial infections and saved hundreds of millions of lives. In animal husbandry, the use of antibacterial drugs has also greatly improved the survival rate of livestock and guaranteed the income of farmers. However, the abuse and misuse of antibacterial drugs, especially antibiotics, have greatly increased the resistance of bacteria in the environment, resulting in bacterial infections that cannot be effectively cured. At present, "super bacteria" that are resistant to various antibiotics and other drugs have appeared around the world. Seriously threaten the health of humans, animals, and the environment. What's more, drug-resistant genes can u...
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Login to View More IPC IPC(8): C12Q1/10C12Q1/04C12R1/19
CPCC12Q1/10C12Q1/04G01N2333/245
Inventor 林文芳崔丽杨凯朱永官
Owner INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI